Featured Publications
Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution
Hao X, Allgeyer ES, Lee DR, Antonello J, Watters K, Gerdes JA, Schroeder LK, Bottanelli F, Zhao J, Kidd P, Lessard MD, Rothman JE, Cooley L, Biederer T, Booth MJ, Bewersdorf J. Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution. Nature Methods 2021, 18: 688-693. PMID: 34059828, PMCID: PMC7610943, DOI: 10.1038/s41592-021-01149-9.Peer-Reviewed Original ResearchConceptsAdaptive optics schemeBest possible spatial resolutionPossible spatial resolutionOptical nanoscopyOptics schemeDiffraction limitDark stateOptical aberrationsNanoscopy methodsThin samplesThick specimenFluorescent moleculesSpatial resolutionThick specimensNanoscopeIsotropic resolutionResolutionNanoscopyPath geometryFluorescence microscopyMicroscopeMicroscopyLight microscopy of proteins in their ultrastructural context
M’Saad O, Bewersdorf J. Light microscopy of proteins in their ultrastructural context. Nature Communications 2020, 11: 3850. PMID: 32737322, PMCID: PMC7395138, DOI: 10.1038/s41467-020-17523-8.Peer-Reviewed Original ResearchConceptsUltrastructural contextLocal protein densityConventional confocal microscopeCellular nanoarchitectureOptical contrastSpecific proteinsElectron microscopyFluorescence microscopyLight microscopyProteinBulk labelingProtein densityConfocal microscopeStandard light microscopyIntracellular spaceMicroscopyProteomeCellsNanoscaleMicroscopeNew principleDiscoveryLabelingPhysical expansionDensity
2021
3D super-resolution deep-tissue imaging in living mice
Velasco MGM, Zhang M, Antonello J, Yuan P, Allgeyer ES, May D, M’Saad O, Kidd P, Barentine AES, Greco V, Grutzendler J, Booth MJ, Bewersdorf J. 3D super-resolution deep-tissue imaging in living mice. Optica 2021, 8: 442-450. PMID: 34239948, PMCID: PMC8243577, DOI: 10.1364/optica.416841.Peer-Reviewed Original ResearchWater-immersion objective lensTwo-photon excitationSuper-resolution imagingEmission depletion (STED) microscopyAdaptive opticsSTED systemObjective lensOptical aberrationsDepletion microscopyBiological tissuesNanoscale structuresLiving mouseOrganic dyesOpticsSTEDExcitationMouse brain tissueLiving cellsThree-dimensional visualizationMicroscopyLightUnique insightsImagingLens
2020
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging
Zhang Y, Schroeder LK, Lessard MD, Kidd P, Chung J, Song Y, Benedetti L, Li Y, Ries J, Grimm JB, Lavis LD, De Camilli P, Rothman JE, Baddeley D, Bewersdorf J. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. Nature Methods 2020, 17: 225-231. PMID: 31907447, PMCID: PMC7028321, DOI: 10.1038/s41592-019-0676-4.Peer-Reviewed Original ResearchConceptsSuper-resolution microscopeProtein complexesNanoscale resolutionSubcellular architectureMammalian cellsCellular organellesPlasma membraneGolgi apparatusEndoplasmic reticulumElectron microscopyThree-dimensional imagingFluorescence approachMulticolor imagingSpatial organizationLocalization precisionFluorescence imagingMolecular specificityFluorescence imagesMicroscopeFluorescent probeOrganellesReticulumMicroscopyImagingMembrane