2003
Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon
Krishnan S, Rajendran VM, Binder HJ. Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon. American Journal Of Physiology - Cell Physiology 2003, 285: c1246-c1254. PMID: 12878493, DOI: 10.1152/ajpcell.00598.2002.Peer-Reviewed Original Research
2001
Na+-dependent fluid absorption in intact perfused rat colonic crypts
Geibel J, Rajendran V, Binder H. Na+-dependent fluid absorption in intact perfused rat colonic crypts. Gastroenterology 2001, 120: 144-150. PMID: 11208723, DOI: 10.1053/gast.2001.20890.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesChloridesColonGanglionic StimulantsGlutamatesIn Vitro TechniquesIntestinal AbsorptionInulinIsotonic SolutionsMalePerfusionQuaternary Ammonium CompoundsRatsRats, Sprague-DawleyRinger's SolutionSodiumSodium-Hydrogen ExchangersTritiumConceptsNet fluid absorptionRat colonic cryptsCl- channel blockerFluid absorptionNeurohumoral stimulationChannel blockersColonic cryptsLumen ClAbsorptive processesInhibitor of NaCl(-) secretionAbstractTextCrypt cellsExchange inhibitorMicroperfusion techniqueFluid secretionL DIDSAmiloride analoguesMammalian colonSecretionAnion exchange inhibitorCryptsSignificant increaseAIMSSecretory process
1999
Role of Cl channels in Cl-dependent Na/H exchange
Rajendran V, Geibel J, Binder H. Role of Cl channels in Cl-dependent Na/H exchange. American Journal Of Physiology 1999, 276: g73-g78. PMID: 9886981, DOI: 10.1152/ajpgi.1999.276.1.g73.Peer-Reviewed Original ResearchConceptsNa/H exchangeCl- channel blockerChannel blockersMicroM DIDSNa/H exchange activityCystic fibrosis transmembrane conductance regulatorIntracellular pH recoveryRat distal colonCl- channelsH exchange activityDistal colonCrypt lumenCrypt cellsAcid loadExchange inhibitorApical membranePH recoveryCl(-) dependenceAnion exchange inhibitorCl- channel activityChannel activityBlockersH exchangePolyclonal antibodiesCl- conductance
1995
An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts.
Singh S, Binder H, Geibel J, Boron W. An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 11573-11577. PMID: 8524806, PMCID: PMC40444, DOI: 10.1073/pnas.92.25.11573.Peer-Reviewed Original ResearchFluid absorption in isolated perfused colonic crypts.
Singh S, Binder H, Boron W, Geibel J. Fluid absorption in isolated perfused colonic crypts. Journal Of Clinical Investigation 1995, 96: 2373-2379. PMID: 7593625, PMCID: PMC185889, DOI: 10.1172/jci118294.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell PolarityColonCyclic AMPIn Vitro TechniquesIntestinal AbsorptionIntestinal MucosaMalePerfusionRatsRats, Sprague-DawleyConceptsCrypt cellsNM vasoactive intestinal peptideFluid absorptionLarge intestinal functionColonic cryptsVasoactive intestinal peptideLamina propria cellsNet fluid secretionRat distal colonNet fluid absorptionDibutyryl cyclic AMPIntestinal peptideDistal colonIntestinal functionLamina propriaPathophysiological paradigmCrypt preparationMM acetylcholineBasal stateNeurohumoral agonistsFluid secretionCellular elementsEpithelial cellsIntestinal cryptsCryptsChloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*
Rajendran V, Geibel J, Binder H. Chloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*. Journal Of Biological Chemistry 1995, 270: 11051-11054. PMID: 7744735, DOI: 10.1074/jbc.270.19.11051.Peer-Reviewed Original ResearchConceptsNa-H exchangeColonic crypt cellsCrypt cellsAcid loadRat distal colonApical membrane vesiclesNovel transport processRate of intracellularDistal colonMM amilorideSodium transportApical membraneGradient stimulationColonic cryptsAmiloride inhibitionSodium movementNovel mechanismOutward gradientCompelling evidenceCellsSodiumUptakePrior studiesColonRecovery
1994
Differential modulation of Na-HCO3 cotransport and Na-H exchange by pH in basolateral membrane vesicles of rat distal colon.
Rajendran V, Binder H. Differential modulation of Na-HCO3 cotransport and Na-H exchange by pH in basolateral membrane vesicles of rat distal colon. Journal Of Biological Chemistry 1994, 269: 156-160. PMID: 8276790, DOI: 10.1016/s0021-9258(17)42328-3.Peer-Reviewed Original ResearchConceptsMembrane vesiclesDifferent cellular functionsBasolateral membrane vesiclesProton concentration gradientCellular functionsIntravesicular protonsApparent KmVesiclesTransport systemNa-H exchangeCotransportDifferential modulationHigh proton concentrationProton concentrationProton stimulationNa-HCO3 cotransportConcentration gradientKmRat distal colonProteinSeparate mechanismsVmax
1993
Mechanism of leukotriene D4 stimulation of Cl- secretion in rat distal colon in vitro
Hyun C, Binder H. Mechanism of leukotriene D4 stimulation of Cl- secretion in rat distal colon in vitro. American Journal Of Physiology 1993, 265: g467-g473. PMID: 8214069, DOI: 10.1152/ajpgi.1993.265.3.g467.Peer-Reviewed Original ResearchConceptsEffect of LTD4Inflammatory bowel diseaseLeukotriene D4Specific LTD4 receptor antagonistLamina propria cellsMediators of inflammationLTD4 receptor antagonistRat distal colonRat colonic mucosaElectrogenic Cl- secretionLTD4 effectsVoltage-clamp conditionsBowel diseaseSerotonergic receptorsReceptor antagonistCyclooxygenase metabolitesDistal colonColonic mucosaCyclooxygenase inhibitorSerosal additionCotransport inhibitorMaximal responseCl(-) secretionLipoxygenase metabolitesArachidonic acidInhibition of VIP-stimulated ion transport by a novel Y-receptor phenotype in rabbit distal colon
Ballantyne G, Goldenring J, Fleming F, Rush S, Flint J, Fielding L, Binder H, Modlin I. Inhibition of VIP-stimulated ion transport by a novel Y-receptor phenotype in rabbit distal colon. American Journal Of Physiology 1993, 264: g848-g854. PMID: 8388641, DOI: 10.1152/ajpgi.1993.264.5.g848.Peer-Reviewed Original ResearchConceptsVIP-stimulated increasesPeptide YYNeuropeptide YPancreatic polypeptideRabbit distal colonDistal colonRabbit distal colonic mucosaBasal short-circuit currentTetrodotoxin-insensitive mechanismVasoactive intestinal polypeptideDistal colonic mucosaColonic secretionIntestinal polypeptideReceptor phenotypeColonic mucosaParacrine regulatorVIP stimulationUssing chambersTissue cAMPPotent inhibitionCl fluxCyclic monophosphateColonInhibitionStimulation
1992
Mucosal ouabain and Na+ inhibit active Rb+(K+) absorption in normal and sodium-depleted rat distal colon
Pandiyan V, Rajendran V, Binder H. Mucosal ouabain and Na+ inhibit active Rb+(K+) absorption in normal and sodium-depleted rat distal colon. Gastroenterology 1992, 102: 1846-1853. PMID: 1587404, DOI: 10.1016/0016-5085(92)90304-h.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsColonIn Vitro TechniquesIntestinal AbsorptionIntestinal MucosaMaleOuabainPotassiumRatsRats, Inbred StrainsRubidium RadioisotopesSodium
1991
Sodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport.
Rajendran V, Oesterlin M, Binder H. Sodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport. Journal Of Clinical Investigation 1991, 88: 1379-1385. PMID: 1655829, PMCID: PMC295609, DOI: 10.1172/jci115444.Peer-Reviewed Original ResearchMechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon
Mascolo N, Rajendran V, Binder H. Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon. Gastroenterology 1991, 101: 331-338. PMID: 2065907, DOI: 10.1016/0016-5085(91)90008-9.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsAnionsBicarbonatesBiological Transport, ActiveButyratesButyric AcidCell MembraneColonFatty Acids, VolatileHydrogen-Ion ConcentrationIn Vitro TechniquesIntestinal AbsorptionRatsRats, Inbred Strains
1990
Active potassium absorption in rat distal colon.
Sweiry J, Binder H. Active potassium absorption in rat distal colon. The Journal Of Physiology 1990, 423: 155-170. PMID: 2143781, PMCID: PMC1189751, DOI: 10.1113/jphysiol.1990.sp018016.Peer-Reviewed Original ResearchConceptsNa-depleted animalsRat distal colonActive potassium absorptionDistal colonNormal colonFree Ringer solutionGroups of animalsResult of inhibitionColonMucosal ouabainPotassium absorptionRinger's solutionSerosal solutionSerosalAnimalsApical membraneSaturable fluxMucosaAffinity constantsOmeprazoleATPaseSecretion
1989
Short-chain fatty acids stimulate active sodium and chloride absorption in vitro in the rat distal colon
Binder H, Mehta P. Short-chain fatty acids stimulate active sodium and chloride absorption in vitro in the rat distal colon. Gastroenterology 1989, 96: 989-996. PMID: 2925072, DOI: 10.1016/0016-5085(89)91614-4.Peer-Reviewed Original ResearchConceptsRat distal colonShort-chain fatty acidsCl absorptionDistal colonNa absorptionNet NaFatty acid stimulationNa-H exchangeHCO3- Ringer's solutionStimulation of NaColonic NaNet Cl- absorptionVoltage-clamp conditionsCarbonic anhydrase inhibitorsFatty acidsCoupling of NaMucosal amilorideChloride absorptionUnidirectional 22NaClamp conditionsButyrate stimulationStimulationColonActive sodiumAcid stimulation
1988
Electrolyte transport in distal colon of sodium-depleted rats: effect of sodium repletion
Turnamian S, Binder H. Electrolyte transport in distal colon of sodium-depleted rats: effect of sodium repletion. American Journal Of Physiology 1988, 255: g329-g338. PMID: 2844099, DOI: 10.1152/ajpgi.1988.255.3.g329.Peer-Reviewed Original ResearchConceptsElectrogenic sodium absorptionElectroneutral sodium chloride absorptionPlasma aldosterone levelsAldosterone levelsSodium chloride absorptionPotassium secretionSodium absorptionSodium repletionDistal colonChloride absorptionElevated plasma aldosterone levelsSodium depletion increasesDistal colonic mucosaSodium-depleted ratsElectroneutral sodium absorptionRat distal colonExcess aldosteroneVoltage-clamp conditionsDietary sodiumColonic mucosaNormal animalsUnidirectional 22NaATPase activityElectrolyte transportSecretion
1987
Aldosterone increases the maximal turnover rate of the sodium pump
Halevy J, Boulpaep E, Binder H, Hayslett J. Aldosterone increases the maximal turnover rate of the sodium pump. Pflügers Archiv - European Journal Of Physiology 1987, 410: 476-480. PMID: 3432052, DOI: 10.1007/bf00586528.Peer-Reviewed Original ResearchConceptsSodium pumpSodium transportLow sodium dietEffects of aldosteroneAdministration of aldosteroneHigher plasma levelsIntact adrenal glandsRat distal colonEquivalent short-circuit currentAddition of nystatinH aldosteroneSodium dietAdrenal glandChronic conditionsPlasma levelsDistal colonAcute actionsAldosteroneUssing chambersHours of exposureChronic exposureSame time periodApical barrierSimilar changesMucosal solution
1986
Electrophysiology of the human colon: evidence of segmental heterogeneity.
Sandle G, Wills N, Alles W, Binder H. Electrophysiology of the human colon: evidence of segmental heterogeneity. Gut 1986, 27: 999. PMID: 3758827, PMCID: PMC1433802, DOI: 10.1136/gut.27.9.999.Peer-Reviewed Original Research
1981
THE EFFECT OF SOMATOSTATIN AND ENKEPHALIN ON ION TRANSPORT IN THE INTESTINE*
Dobbins J, Dharmsathaphorn K, Racusen L, Binder H. THE EFFECT OF SOMATOSTATIN AND ENKEPHALIN ON ION TRANSPORT IN THE INTESTINE*. Annals Of The New York Academy Of Sciences 1981, 372: 594-612. PMID: 6122417, DOI: 10.1111/j.1749-6632.1981.tb15508.x.Peer-Reviewed Original ResearchConceptsEffect of somatostatinFinal common pathwayCa channel blockersCa-free solutionCyclic AMP levelsInflux of NaCarcinoid syndromeIntestinal motilityAntidiarrheal effectSRIF infusionSecretory diarrheaRat colonRabbit ileumAMP levelsElectrolyte absorptionStimulatory effectC1 absorptionEnkephalinDistal stepBrush border membraneCommon pathwayDiarrheaCyclic AMPNet NaSRIF
1974
Intestinal Oxalate Absorption
Binder H. Intestinal Oxalate Absorption. Gastroenterology 1974, 67: 441-446. PMID: 4852527, DOI: 10.1016/s0016-5085(19)32846-x.Peer-Reviewed Original ResearchConceptsOxalate absorptionIntestinal oxalate absorptionIntestinal oxalate transportIleal diseaseIleal dysfunctionDietary oxalateIntestinal absorptionMucosal accumulationRabbit ileumUptake of oxalateRat duodenumNonsaturable processOxalate solubilityIleumOxalate transportMetabolic inhibitorsRate of uptakeUptakePatientsHyperoxaluriaActive transportOxalate concentrationCholestyramineDysfunctionDuodenum