2023
APOBEC-1 deletion enhances cisplatin-induced acute kidney injury
Guo X, Blanc V, Davidson N, Velazquez H, Chen T, Moledina D, Moeckel G, Safirstein R, Desir G. APOBEC-1 deletion enhances cisplatin-induced acute kidney injury. Scientific Reports 2023, 13: 22255. PMID: 38097707, PMCID: PMC10721635, DOI: 10.1038/s41598-023-49575-3.Peer-Reviewed Original Research
2022
Kidney-Targeted Renalase Agonist Prevents Cisplatin-Induced Chronic Kidney Disease by Inhibiting Regulated Necrosis and Inflammation
Guo X, Xu L, Velazquez H, Chen TM, Williams RM, Heller DA, Burtness B, Safirstein R, Desir GV. Kidney-Targeted Renalase Agonist Prevents Cisplatin-Induced Chronic Kidney Disease by Inhibiting Regulated Necrosis and Inflammation. Journal Of The American Society Of Nephrology 2022, 33: 342-356. PMID: 34921111, PMCID: PMC8819981, DOI: 10.1681/asn.2021040439.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntineoplastic AgentsCell LineCisplatinCreatinineDisease Models, AnimalGene ExpressionGlomerular Filtration RateHepatitis A Virus Cellular Receptor 1HumansKidneyMiceMice, Inbred C57BLMice, KnockoutMonoamine OxidaseNanocapsulesPeptidesRenal Insufficiency, ChronicConceptsRenal proximal tubulesSingle-cell RNA sequencing analysisMesoscale nanoparticlesFirst doseCisplatin chemotherapyProximal tubulesAgonist peptideInduced Chronic Kidney DiseaseGenetic deletionNeck squamous cell carcinomaRNA sequencing analysisCisplatin-induced AKIKidney-targeted deliveryChronic kidney diseaseDevelopment of CKDSquamous cell carcinomaAdministration of cisplatinPlasma renalaseAdvanced headCell carcinomaInflammatory cytokinesKidney diseasePlasma creatinineSystemic administrationRegulated necrosis
2016
Renalase Expression by Melanoma and Tumor-Associated Macrophages Promotes Tumor Growth through a STAT3-Mediated Mechanism
Hollander L, Guo X, Velazquez H, Chang J, Safirstein R, Kluger H, Cha C, Desir G. Renalase Expression by Melanoma and Tumor-Associated Macrophages Promotes Tumor Growth through a STAT3-Mediated Mechanism. Cancer Research 2016, 76: 3884-3894. PMID: 27197188, PMCID: PMC5031238, DOI: 10.1158/0008-5472.can-15-1524.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBiomarkers, TumorBlotting, WesternCase-Control StudiesCell CycleCell ProliferationFemaleFollow-Up StudiesGene Expression Regulation, NeoplasticHumansImmunoenzyme TechniquesMacrophagesMaleMelanomaMiceMice, Inbred C57BLMice, NudeMonoamine OxidaseNeoplasm StagingP38 Mitogen-Activated Protein KinasesPrognosisProto-Oncogene Proteins c-aktSignal TransductionSTAT3 Transcription FactorSurvival RateTumor Cells, CulturedXenograft Model Antitumor AssaysConceptsTumor-associated macrophagesDisease-specific survivalManagement of melanomaPotential therapeutic implicationsCell cycle inhibitor p21Melanoma cell growthPI3K/AktMelanoma cell survivalCell growth arrestPathogenic rolePrimary melanomaToxic injuryMurine xenograftsTherapeutic implicationsTumor growthClinical specimensRenalaseBax activationTumor microenvironmentTumor cellsInhibitor p21Growth arrestSurvival factorElevated expressionMAPK pathway
2014
Renalase regulates peripheral and central dopaminergic activities
Quelhas-Santos J, Serrão MP, Soares-Silva I, Fernandes-Cerqueira C, Simões-Silva L, Pinho MJ, Remião F, Sampaio-Maia B, Desir GV, Pestana M. Renalase regulates peripheral and central dopaminergic activities. American Journal Of Physiology. Renal Physiology 2014, 308: f84-f91. PMID: 25411385, PMCID: PMC4338928, DOI: 10.1152/ajprenal.00274.2014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainDopamineDopaminergic NeuronsJejunumKidneyMaleMice, Inbred C57BLMice, KnockoutMonoamine OxidaseConceptsKO miceUrinary excretionPlasma levelsDopaminergic activityIncreased Plasma LevelsPeripheral dopaminergic activityUrine catecholamine levelsRenal dopaminergic systemCentral dopaminergic activityL-type amino acid transporterWild-type miceAmino acid decarboxylase activityKnockout mouse modelRenalase deficiencyCatecholamine levelsDA outputUrinary dopamineAADC activityDopaminergic systemRenal cortexMouse modelDOPA ratioVivo administrationOverexpression of LAT1Amino acid transportersRenalase Prevents AKI Independent of Amine Oxidase Activity
Wang L, Velazquez H, Moeckel G, Chang J, Ham A, Lee HT, Safirstein R, Desir GV. Renalase Prevents AKI Independent of Amine Oxidase Activity. Journal Of The American Society Of Nephrology 2014, 25: 1226-1235. PMID: 24511138, PMCID: PMC4033373, DOI: 10.1681/asn.2013060665.Peer-Reviewed Original ResearchConceptsIschemic injuryCatecholamine levelsRecombinant renalaseAmine oxidase activityHuman proximal tubular cellsCisplatin-induced AKITreatment of AKIWild-type miceHK-2 cellsProximal tubular cellsOxidase activityKidney injuryRenal injuryC-Jun N-terminal kinaseExtracellular signal-regulated kinaseP38 mitogen-activated protein kinaseToxic injuryRenalase proteinTubular cellsSignal-regulated kinaseIntracellular signaling cascadesRenalaseInjuryMitogen-activated protein kinaseN-terminal kinase
2013
Renalase Protects against Ischemic AKI
Lee HT, Kim JY, Kim M, Wang P, Tang L, Baroni S, D’Agati V, Desir GV. Renalase Protects against Ischemic AKI. Journal Of The American Society Of Nephrology 2013, 24: 445-455. PMID: 23393318, PMCID: PMC3582209, DOI: 10.1681/asn.2012090943.Peer-Reviewed Original ResearchMeSH KeywordsAcute Kidney InjuryAdrenergic alpha-AntagonistsAnimalsApoptosisGene ExpressionHumansInflammation MediatorsIschemiaKidney Tubular Necrosis, AcuteMacrophagesMaleMiceMice, Inbred C57BLMice, KnockoutMonoamine OxidaseNeutrophil InfiltrationNorepinephrinePhentolamineRecombinant ProteinsReperfusion InjuryRNA, MessengerConceptsRenal ischemia-reperfusion injuryIschemia-reperfusion injuryIschemic AKIWild-type miceReperfusion injuryCatecholamine levelsRenal tubular inflammationTreatment of AKIRenal ischemia reperfusionSham-operated micePlasma catecholamine levelsRenal tubular necrosisRecombinant human renalasePlasma renalaseTubular inflammationTubular necrosisIschemia reperfusionNE levelsPlasma catecholaminesMyocardial necrosisInflammatory responseProximal tubulesAKIRenalaseMice
2011
Increased renal dopamine and acute renal adaptation to a high-phosphate diet
Weinman EJ, Biswas R, Steplock D, Wang P, Lau YS, Desir GV, Shenolikar S. Increased renal dopamine and acute renal adaptation to a high-phosphate diet. American Journal Of Physiology. Renal Physiology 2011, 300: f1123-f1129. PMID: 21325500, PMCID: PMC3094044, DOI: 10.1152/ajprenal.00744.2010.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnalysis of VarianceAnimalsAromatic Amino Acid Decarboxylase InhibitorsCarbidopaCyclic AMP-Dependent Protein KinasesDopa DecarboxylaseDopamineEnzyme InhibitorsKidneyMaleMiceMice, Inbred C57BLMonoamine OxidasePhosphorus, DietaryProtein Kinase CRatsRats, Sprague-DawleySignal TransductionTime FactorsUp-RegulationConceptsHigh-phosphate dietLow-phosphate dietPhosphate excretionDopamine contentRenal dopamine receptorsRenal phosphate excretionRenal tubular reabsorptionTreatment of ratsMarkers of activationRole of dopamineRenal dopamineUrinary excretionTubular reabsorptionRenal adaptationTwo- to threefold increaseAcute increaseMonoamine oxidase ADopamine receptorsDopamine synthesisImpaired adaptation
2010
Renalase deficiency aggravates ischemic myocardial damage
Wu Y, Xu J, Velazquez H, Wang P, Li G, Liu D, Sampaio-Maia B, Quelhas-Santos J, Russell K, Russell R, Flavell RA, Pestana M, Giordano F, Desir GV. Renalase deficiency aggravates ischemic myocardial damage. Kidney International 2010, 79: 853-860. PMID: 21178975, DOI: 10.1038/ki.2010.488.Peer-Reviewed Original ResearchConceptsChronic kidney diseaseWild-type miceRenalase deficiencyKnockout micePlasma blood urea nitrogenLevels of renalaseMild ventricular hypertrophyRenalase knockout mouseNormal systolic functionTraditional risk factorsPlasma catecholamine levelsIschemic myocardial damageBlood urea nitrogenCardiac complicationsCardiovascular complicationsSystolic functionVentricular hypertrophyCardioprotective effectsCatecholamine levelsKidney diseaseMyocardial damageMyocardial necrosisRecombinant renalaseRisk factorsCardiac ischemia
2007
Regulation of insulin secretion and GLUT4 trafficking by the calcium sensor synaptotagmin VII
Li Y, Wang P, Xu J, Gorelick F, Yamazaki H, Andrews N, Desir GV. Regulation of insulin secretion and GLUT4 trafficking by the calcium sensor synaptotagmin VII. Biochemical And Biophysical Research Communications 2007, 362: 658-664. PMID: 17720139, PMCID: PMC2194288, DOI: 10.1016/j.bbrc.2007.08.023.Peer-Reviewed Original ResearchConceptsGLUT4 trafficSyt VIIPlasma membraneGLUT4 translocationConstitutive expressionSecretory granule exocytosisSkeletal muscle cellsGLUT4 traffickingRegulated exocytosisVoltage-gated potassium channel Kv1.3Vesicular trafficSynaptotagmin VIIGLUT4 presentPotassium channel Kv1.3Calcium sensorIntracellular compartmentsDeletion resultsGlucose-stimulated insulin secretionChannel Kv1.3Granule exocytosisPancreatic beta cellsChannel activityInsulin secretionPancreatic islet cellsMuscle cells
2004
The voltage-gated potassium channel Kv1.3 regulates peripheral insulin sensitivity
Xu J, Wang P, Li Y, Li G, Kaczmarek LK, Wu Y, Koni PA, Flavell RA, Desir GV. The voltage-gated potassium channel Kv1.3 regulates peripheral insulin sensitivity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 3112-3117. PMID: 14981264, PMCID: PMC365752, DOI: 10.1073/pnas.0308450100.Peer-Reviewed Original ResearchMeSH KeywordsAdipose TissueAnimalsBiological TransportFastingGlucoseInsulinInterleukin-6JNK Mitogen-Activated Protein KinasesKineticsKv1.3 Potassium ChannelMaleMiceMice, Inbred C57BLMice, KnockoutMice, ObeseMitogen-Activated Protein KinasesModels, BiologicalMuscle, SkeletalPotassium ChannelsPotassium Channels, Voltage-GatedTumor Necrosis Factor-alphaConceptsKv1.3-/- micePeripheral glucose homeostasisPeripheral insulin sensitivityPlasma membraneGene inactivationInsulin sensitivityAmount of GLUT4Skeletal muscleTerminal kinase (JNK) activityGlucose homeostasisAdipose tissueLower blood insulin levelsVoltage-gated potassium channelsInsulin-stimulated glucose uptakeVoltage-gated potassium channel Kv1.3Tumor necrosis factor productionExperimental autoimmune encephalitisBlood insulin levelsHigh-fat dietPotassium channel Kv1.3Tumor necrosis factor secretionPeripheral T lymphocytesKinase activityNecrosis factor productionNumber of tissues