2012
Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism
St.Onge R, Schlecht U, Scharfe C, Evangelista M. Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism. Molecules 2012, 17: 13098-13115. PMID: 23128089, PMCID: PMC3539408, DOI: 10.3390/molecules171113098.Peer-Reviewed Original ResearchConceptsChemical geneticsChemical probesCellular metabolismDominant model organismChemical genetic screeningForward chemical geneticsHigh-throughput phenotypicDrug target identificationNormal cellular metabolismNew chemical probesHigher eukaryotesExperimental tractabilityModel organismsYeast SaccharomycesCellular processesIdeal organismNew druggable targetsMolecular biologyYeastDruggable targetsGeneticsDiseased statesOrganismsGenetic screeningMetabolism
2002
Evolutionary Rate in the Protein Interaction Network
Fraser HB, Hirsh AE, Steinmetz LM, Scharfe C, Feldman MW. Evolutionary Rate in the Protein Interaction Network. Science 2002, 296: 750-752. PMID: 11976460, DOI: 10.1126/science.1068696.Peer-Reviewed Original ResearchConceptsProtein interaction networksInteraction networksMost cellular functionsRate of evolutionHigh-throughput screenMolecular evolutionEvolutionary ratesYeast SaccharomycesCellular functionsEvolutionary changeSelection pressureProtein resultsProteinMore interactorsInteractorsFundamental questionsSaccharomycesCoevolutionEvolutionOrganismsReciprocal changesFunctionSimilar ratesGreater proportionScreen