1999
The Second Hydrophobic Domain Contributes to the Kinetic Properties of Epithelial Sodium Channels*
Fyfe G, Zhang P, Canessa C. The Second Hydrophobic Domain Contributes to the Kinetic Properties of Epithelial Sodium Channels*. Journal Of Biological Chemistry 1999, 274: 36415-36421. PMID: 10593937, DOI: 10.1074/jbc.274.51.36415.Peer-Reviewed Original ResearchConceptsSecond hydrophobic domainEpithelial sodium channelBeta subunitHydrophobic domainWild-type subunitsSecond transmembrane domainENaC/Deg familyTransmembrane domainChimeric subunitsSodium channelsFunctional poresSubunit alphaAlpha subunitKinetic propertiesFunctional channelsSubunitsSingle-channel conductanceIon channelsSpecific sequencesXenopus oocytesSmall conductanceOpen probabilityChannel conductanceFunctional propertiesAmiloride affinity
1994
Membrane topology of the epithelial sodium channel in intact cells
Canessa C, Merillat A, Rossier B. Membrane topology of the epithelial sodium channel in intact cells. American Journal Of Physiology 1994, 267: c1682-c1690. PMID: 7810611, DOI: 10.1152/ajpcell.1994.267.6.c1682.Peer-Reviewed Original ResearchConceptsLarge hydrophilic loopHydrophilic loopIntact cellsMembrane topologyEpithelial sodium channelPutative transmembrane domainsStop-transfer signalAmiloride-sensitive epithelial sodium channelCell-free translation assaysShort NH2Transmembrane domainMembrane insertionHomologous subunitsXenopus laevis oocytesTranslation assaysSodium channelsGlycosylation sitesCOOH terminusCytoplasmic sideFunctional expressionTerminal endSubunitsHydrophilic NH2Laevis oocytesAlpha-rENaCChapter 4 Structure–Function Relationship of Na,K-ATPase: The Digitalis Receptor
Canessa C, Jaisser F, Horisberger J, Rossier B. Chapter 4 Structure–Function Relationship of Na,K-ATPase: The Digitalis Receptor. Current Topics In Membranes 1994, 41: 71-85. DOI: 10.1016/s0070-2161(08)60454-2.Peer-Reviewed Original ResearchStructure-function relationshipsAmino acidsFirst transmembrane domainSite-directed mutagenesisK-ATPaseAmino acid residuesOuabain-sensitive cellsFunctional mutantsTransmembrane domainCellular processesDrug-binding sitesAromatic amino acidsDigitalis receptorWild typeContact sitesRandom mutationsOuabain resistanceΑ-subunitAcid residuesSite-specific ligandsFunctional assaysCardiac glycosidesCell linesOuabain analoguesK-ATPase activity