2012
High‐Efficiency Transfection and siRNA‐Mediated Gene Knockdown in Human Pluripotent Stem Cells
Ma Y, Lin H, Qiu C. High‐Efficiency Transfection and siRNA‐Mediated Gene Knockdown in Human Pluripotent Stem Cells. Current Protocols In Stem Cell Biology 2012, 21: 5c.2.1-5c.2.9. PMID: 22605647, DOI: 10.1002/9780470151808.sc05c02s21.Peer-Reviewed Original ResearchConceptsHigh transfection efficiencyPluripotent stem cellsTransfection efficiencyTransfection reagent Lipofectamine 2000Human embryonic stem cellsStem cellsHuman pluripotent stem cellsEmbryonic stem cellsPluripotent cell linesLipofectamine 2000SiRNA-mediated gene knockdownPluripotent cellsExpensive equipmentPluripotent genesGene knockdownProtocolEfficiencyPrimary cellsGenesCell linesCarriersCellsApplicationsTransfection
2009
Complex developmental patterns of histone modifications associated with the human β-globin switch in primary cells
Hsu M, Richardson CA, Olivier E, Qiu C, Bouhassira EE, Lowrey CH, Fiering S. Complex developmental patterns of histone modifications associated with the human β-globin switch in primary cells. Experimental Hematology 2009, 37: 799-806.e4. PMID: 19460472, PMCID: PMC2748252, DOI: 10.1016/j.exphem.2009.04.006.Peer-Reviewed Original ResearchConceptsHistone modificationsGene expressionBeta-globin switchDimethyl lysine 9Beta-globin locusΒ-globin switchGamma geneImportant histone modificationsBone marrow cellsGamma gene expressionSuch histonesComplex developmental patternLysine 9Chromatin immunoprecipitationHistone H3Gene suppressionPrimary fetalMarrow cellsUnexpressed genesGamma-globinGenesHistonesDevelopmental stagesBone marrow erythroblastsPrimary cells