2007
Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery
Pearse DD, Sanchez AR, Pereira FC, Andrade CM, Puzis R, Pressman Y, Golden K, Kitay BM, Blits B, Wood PM, Bunge MB. Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia 2007, 55: 976-1000. PMID: 17526000, DOI: 10.1002/glia.20490.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAxonsBrain Tissue TransplantationCell CommunicationCell MovementCell SurvivalFemaleGenetic MarkersGenotypeGraft SurvivalGreen Fluorescent ProteinsMaleNeural PathwaysOlfactory BulbRatsRats, Inbred F344Recovery of FunctionSchwann CellsSpinal Cord InjuriesTreatment OutcomeY ChromosomeConceptsContused spinal cordSchwann cellsSpinal cordFunctional recoveryAdult rat spinal cordGrafted Schwann cellsSpinal cord injury repairRat spinal cordLentiviral vector-mediated expressionNumerous myelinated axonsLong-term markersVector-mediated expressionInjury milieuHost axonsMale transplantsGrafted cellsFemale ratsInjury siteCentral canalInjury repairY chromosome-specific probesMyelinated axonsSurvival rateLesionsTransplanted cells
2005
Lentiviral vector-mediated transduction of neural progenitor cells before implantation into injured spinal cord and brain to detect their migration, deliver neurotrophic factors and repair tissue.
Blits B, Kitay BM, Farahvar A, Caperton CV, Dietrich WD, Bunge MB. Lentiviral vector-mediated transduction of neural progenitor cells before implantation into injured spinal cord and brain to detect their migration, deliver neurotrophic factors and repair tissue. Restorative Neurology And Neuroscience 2005, 23: 313-24. PMID: 16477093.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain InjuriesCell MovementCells, CulturedDisease Models, AnimalEmbryo, MammalianFemaleGenetic VectorsGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsImmunohistochemistryIntermediate Filament ProteinsLentivirusNerve Growth FactorsNerve Tissue ProteinsNestinNeuronsRatsRats, Inbred F344Rats, Sprague-DawleySpinal Cord InjuriesStem Cell TransplantationStem CellsTransduction, GeneticTubulinConceptsNeural progenitor cellsSpinal cordNeurotrophic factorPositive cellsGFP-positive cellsContused spinal cordProgenitor cellsSpinal cord lesionsCortical neural progenitor cellsLong-term transgene expressionLentiviral vectorsRat spinal cordLentiviral vector-mediated transductionCord lesionsGreen fluorescent proteinReplacement therapyCell replacement therapyVector-mediated transductionNPC survivalLesion siteAstrocytic phenotypeImmune rejectionCordNeurological disordersNon-transduced cells