1996
Na:K:2Cl Cotransporter (NKCC) of Intestinal Epithelial Cells SURFACE EXPRESSION IN RESPONSE TO cAMP*
D'Andrea L, Lytle C, Matthews J, Hofman P, Forbush B, Madara J. Na:K:2Cl Cotransporter (NKCC) of Intestinal Epithelial Cells SURFACE EXPRESSION IN RESPONSE TO cAMP*. Journal Of Biological Chemistry 1996, 271: 28969-28976. PMID: 8910547, DOI: 10.1074/jbc.271.46.28969.Peer-Reviewed Original ResearchConceptsChloride secretionSurface expressionBumetanide-sensitive NaMonoclonal antibodiesIntestinal chloride secretionIncreased surface expressionCell surface expressionEpithelial cell lineNKCC activityT84 monolayersCotransport activityAgonist stimulationCAMP agonistsEpithelial uptakeCotransporterBasolateral domainIntestinal epitheliaSurface biotinylationNKCCConfocal localizationApproximately 6-foldCell linesF-actin cytoskeletonSecretionImmunoelectron microscopy
1992
The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues.
Torchia J, Lytle C, Pon D, Forbush B, Sen A. The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues. Journal Of Biological Chemistry 1992, 267: 25444-25450. PMID: 1281159, DOI: 10.1016/s0021-9258(19)74061-7.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine8-Bromo Cyclic Adenosine MonophosphateAnimalsBlotting, WesternCalciumCarbacholCarrier ProteinsCell MembraneColforsinCyclic AMPDucksKineticsMembrane ProteinsPhosphatesPhosphorylationSalt GlandSodium-Potassium-Chloride SymportersTime FactorsVasoactive Intestinal PeptideConceptsNa-K-Cl cotransporterPhosphorylation of pp170Avian salt glandMonoclonal antibodiesResponse to carbacholEffect of forskolinNa-K-ClIntact cell preparationsConcentration-dependent increaseStimulation of secretionIncreased approximately 5-foldCAMP accumulationExposure of cellsCAMP-dependent protein kinasePp170Approximately 5-foldCell preparationsCAMP-dependentSalt gland cellsGland membranesWestern blottingForskolinSecretogoguesVIPAntibodies