1994
The molecular basis of chloride transport in shark rectal gland.
Riordan J, Forbush B, Hanrahan J. The molecular basis of chloride transport in shark rectal gland. Journal Of Experimental Biology 1994, 196: 405-418. PMID: 7529818, DOI: 10.1242/jeb.196.1.405.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological Transport, ActiveCarrier ProteinsChloride ChannelsChloridesCystic Fibrosis Transmembrane Conductance RegulatorEpitheliumHomeostasisHumansMembrane ProteinsModels, BiologicalPotassium ChannelsProtein Structure, SecondarySalt GlandSecond Messenger SystemsSharksSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseConceptsApical Cl- channelsCl- channelsProtein kinase ACl- secretionBasolateral transport proteinsTransepithelial Cl- secretionNa+/K(+)-ATPase pumpIntracellular chloride concentrationStimulation of secretionActive Na+ extrusionBasolateral cotransporterShark rectal glandNa+/K+/2Cl- cotransporterCl- movementK+ channelsK+ exitNa+/K(+)-ATPaseApical componentParacellular flowPrimary stimulationEpithelial cellsExcellent experimental modelCotransporterCFTRRectal gland of elasmobranchs
1992
The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues.
Torchia J, Lytle C, Pon D, Forbush B, Sen A. The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues. Journal Of Biological Chemistry 1992, 267: 25444-25450. PMID: 1281159, DOI: 10.1016/s0021-9258(19)74061-7.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine8-Bromo Cyclic Adenosine MonophosphateAnimalsBlotting, WesternCalciumCarbacholCarrier ProteinsCell MembraneColforsinCyclic AMPDucksKineticsMembrane ProteinsPhosphatesPhosphorylationSalt GlandSodium-Potassium-Chloride SymportersTime FactorsVasoactive Intestinal PeptideConceptsNa-K-Cl cotransporterPhosphorylation of pp170Avian salt glandMonoclonal antibodiesResponse to carbacholEffect of forskolinNa-K-ClIntact cell preparationsConcentration-dependent increaseStimulation of secretionIncreased approximately 5-foldCAMP accumulationExposure of cellsCAMP-dependent protein kinasePp170Approximately 5-foldCell preparationsCAMP-dependentSalt gland cellsGland membranesWestern blottingForskolinSecretogoguesVIPAntibodiesNa-K-Cl cotransport in the shark rectal gland. I. Regulation in the intact perfused gland
Forbush B, Haas M, Lytle C. Na-K-Cl cotransport in the shark rectal gland. I. Regulation in the intact perfused gland. American Journal Of Physiology 1992, 262: c1000-c1008. PMID: 1566806, DOI: 10.1152/ajpcell.1992.262.4.c1000.Peer-Reviewed Original ResearchConceptsNa-K-ClNa-K-Cl cotransport systemNa-K-Cl cotransporterVasoactive intestinal peptideCotransport systemVIP-stimulated increasesStimulation of secretionShark rectal glandK exitBasolateral membranePerfused glandShark Squalus acanthiasIntestinal peptideArterial-venous differencesDogfish shark Squalus acanthiasIntact glandsPerfusion solutionPerfusion periodBasal statePerfusionGlandInvestigate regulationRectal glandRinger's solutionTime course