2011
Rare mutations in the human Na-K-Cl cotransporter (NKCC2) associated with lower blood pressure exhibit impaired processing and transport function
Monette MY, Rinehart J, Lifton RP, Forbush B. Rare mutations in the human Na-K-Cl cotransporter (NKCC2) associated with lower blood pressure exhibit impaired processing and transport function. American Journal Of Physiology. Renal Physiology 2011, 300: f840-f847. PMID: 21209010, PMCID: PMC3074999, DOI: 10.1152/ajprenal.00552.2010.Peer-Reviewed Original ResearchConceptsHEK-293 cellsNa-K-Cl cotransporterTransport functionMajor salt transport pathwayPlasma membrane localizationHEK cellsLow transport activitySequence conservationMembrane localizationProtein functionHeterologous expressionXenopus laevis oocytesImportant residuesMutantsRenal salt reabsorptionMolecular mechanismsIndependent mutationsConstitutive activityTransport activityBlood pressureFunctional consequencesImpaired transport functionSuch mutationsProcessing defectsLaevis oocytes
2010
Drosophila glia use a conserved cotransporter mechanism to regulate extracellular volume
Leiserson WM, Forbush B, Keshishian H. Drosophila glia use a conserved cotransporter mechanism to regulate extracellular volume. Glia 2010, 59: 320-332. PMID: 21125654, PMCID: PMC3005002, DOI: 10.1002/glia.21103.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsAnimals, Genetically ModifiedBlood-Nerve BarrierCells, CulturedDrosophilaDrosophila ProteinsExtracellular SpaceHumansIn Vitro TechniquesLarvaMicroscopy, Electron, TransmissionModels, BiologicalMutationNeural ConductionNeurogliaNeuronsPeripheral NervesProtein Serine-Threonine KinasesSymportersTwo-Hybrid System TechniquesConceptsHuman NKCC1Yeast two hybrid assaysExtracellular solute compositionLarvae mutantDrosophila gliaNcc69Osmotic homeostasisExtracellular volumeMolecular mechanismsNervous systemOrthologsExtracellular solutesPhysiological mechanismsBlood-brain barrierBlood-nerve barrierSimilar roleAccumulation of fluidAction potential conductionGlial cellsPeripheral neuropathyNKCC1Serious health threatDetectable impactBlood barrierGlia