Alyssa Blaise
she/her/hers
Postgraduate AssociateAbout
Research
Publications
2022
The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance
Dall’Agnese A, Platt J, Zheng M, Friesen M, Dall’Agnese G, Blaise A, Spinelli J, Henninger J, Tevonian E, Hannett N, Lazaris C, Drescher H, Bartsch L, Kilgore H, Jaenisch R, Griffith L, Cisse I, Jeppesen J, Lee T, Young R. The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance. Nature Communications 2022, 13: 7522. PMID: 36473871, PMCID: PMC9727033, DOI: 10.1038/s41467-022-35176-7.Peer-Reviewed Original Research
2021
In vitro fluorescence assay to measure GDP/GTP exchange of guanine nucleotide exchange factors of Rho family GTPases
Blaise AM, Corcoran EE, Wattenberg ES, Zhang YL, Cottrell JR, Koleske AJ. In vitro fluorescence assay to measure GDP/GTP exchange of guanine nucleotide exchange factors of Rho family GTPases. Biology Methods And Protocols 2021, 7: bpab024. PMID: 35087952, PMCID: PMC8789339, DOI: 10.1093/biomethods/bpab024.Peer-Reviewed Original ResearchNucleotide exchange factorsGEF domainExchange factorGuanine nucleotide exchange factorsGDP/GTP exchangeRho family GEFsActivation of GTPasesFluorescence-based analysisRare variantsGTP loadingGTP exchangeRho familyHigh-throughput screeningWhole-exome sequencingCellular activitiesGenetic variantsGTPasesGEFExome sequencingCritical roleUser-friendly protocolComplete understandingHuman developmentVariantsGEFs
2020
Crystal structure of a guanine nucleotide exchange factor encoded by the scrub typhus pathogen Orientia tsutsugamushi
Lim C, Berk JM, Blaise A, Bircher J, Koleske AJ, Hochstrasser M, Xiong Y. Crystal structure of a guanine nucleotide exchange factor encoded by the scrub typhus pathogen Orientia tsutsugamushi. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 30380-30390. PMID: 33184172, PMCID: PMC7720168, DOI: 10.1073/pnas.2018163117.Peer-Reviewed Original ResearchConceptsBacterial GEFStructure-guided mutational analysisExchange factor domainRho family GTPasesObvious sequence similarityRho GTPases Rac1Pathogen Orientia tsutsugamushiAlters cell morphologyInteraction screenConvergent evolutionGEF domainHost interactorsExchange factorNucleotide displacementGEF activityCellular processesSequence similarityCytoskeletal structuresGTPases Rac1Ectopic expressionMutational analysisGEFRac1Factor domainPathogenic bacterium
2018
Tubular approach to minimally invasive microdiscectomy for pediatric lumbar disc herniation.
Montejo JD, Camara-Quintana JQ, Duran D, Rockefeller JM, Conine SB, Blaise AM, Kahle KT, DiLuna ML. Tubular approach to minimally invasive microdiscectomy for pediatric lumbar disc herniation. Journal Of Neurosurgery Pediatrics 2018, 21: 449-455. PMID: 29473813, DOI: 10.3171/2017.11.peds17293.Peer-Reviewed Original ResearchConceptsPediatric lumbar disc herniationLumbar disc herniationOswestry Disability IndexTubular microdiscectomyPediatric patientsMacNab criteriaDisc herniationTubular retractorPositive straight leg raiseLow back pain scoreLarge prospective cohort studyMedian hospital lengthEpidural steroid injectionsProspective cohort studyResolution of symptomsRetrospective chart reviewConsecutive pediatric patientsLong-term efficacyStraight leg raiseSD operative timeMedian followODI evaluationDisability IndexHospital lengthLeg pain