Vijay Kuchroo
Research
Publications
2025
An IL-27-Driven Transcriptional Network Identifies Regulators of IL-10 Expression across T Helper Cell Subsets
Zhang H, Madi A, Yosef N, Chihara N, Awasthi A, Pot C, Lambden C, Srivastava A, Burkett P, Nyman J, Christian E, Etminan Y, Lee A, Stroh H, Xia J, Karwacz K, Thakore P, Acharya N, Schnell A, Wang C, Apetoh L, Rozenblatt-Rosen O, Anderson A, Regev A, Kuchroo V. An IL-27-Driven Transcriptional Network Identifies Regulators of IL-10 Expression across T Helper Cell Subsets. Cell Reports 2025, 44: 115455. PMID: 40057956, DOI: 10.1016/j.celrep.2025.115455.Peer-Reviewed Original Research
2024
Transcription factor TCF1 binds to RORγt and orchestrates a regulatory network that determines homeostatic Th17 cell state
Mangani D, Subramanian A, Huang L, Cheng H, Krovi S, Wu Y, Yang D, Moreira T, Escobar G, Schnell A, Dixon K, Krishnan R, Singh V, Sobel R, Weiner H, Kuchroo V, Anderson A. Transcription factor TCF1 binds to RORγt and orchestrates a regulatory network that determines homeostatic Th17 cell state. Immunity 2024, 57: 2565-2582.e6. PMID: 39447575, PMCID: PMC11614491, DOI: 10.1016/j.immuni.2024.09.017.Peer-Reviewed Original ResearchConceptsCell statesRegulatory networksSpectrum of cell statesTh17 cellsTranscription factor TPro-inflammatory Th17 cellsHomeostatic tissue functionReceptor signalingMature T cellsAutoimmune tissue damageInterleukin (IL)-23Controlling tissue inflammationPro-inflammatory functionsPro-inflammatory cellsConditional deletionDevelopment of therapiesRestore homeostasisPro-inflammatory potentialTCF1T-helperT cellsRORgtTissue inflammationCellsInflammatory diseasesNeuropeptide signalling orchestrates T cell differentiation
Hou Y, Sun L, LaFleur M, Huang L, Lambden C, Thakore P, Geiger-Schuller K, Kimura K, Yan L, Zang Y, Tang R, Shi J, Barilla R, Deng L, Subramanian A, Wallrapp A, Choi H, Kye Y, Ashenberg O, Schiebinger G, Doench J, Chiu I, Regev A, Sharpe A, Kuchroo V. Neuropeptide signalling orchestrates T cell differentiation. Nature 2024, 635: 444-452. PMID: 39415015, DOI: 10.1038/s41586-024-08049-w.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 3AnimalsCalcitonin Gene-Related PeptideCalcitonin Receptor-Like ProteinCell DifferentiationCyclic AMP Response Element-Binding ProteinFemaleMaleMiceMice, Inbred C57BLReceptor Activity-Modifying Protein 3Signal TransductionSTAT1 Transcription FactorTh1 CellsTh2 CellsConceptsT helper type 1Acute viral infectionActivating transcription factor 3Th1 cell differentiationCAMP response element-binding proteinViral infectionCell differentiationNeuropeptide CGRPFate determinationT cellsCD8+ T cell responsesDifferentiation of Th2 cellsIn vitro polarizationT cell fate determinationT cell responsesTh1 cell responsesCell fate determinationIn vivo CRISPR screeningDownstream cAMP response element-binding proteinT cell differentiationT helper cell differentiationIn vivo differentiationResponse element-binding proteinElement-binding proteinNeuroimmune circuitsBACH2 regulates diversification of regulatory and proinflammatory chromatin states in TH17 cells
Thakore P, Schnell A, Huang L, Zhao M, Hou Y, Christian E, Zaghouani S, Wang C, Singh V, Singaraju A, Krishnan R, Kozoriz D, Ma S, Sankar V, Notarbartolo S, Buenrostro J, Sallusto F, Patsopoulos N, Rozenblatt-Rosen O, Kuchroo V, Regev A. BACH2 regulates diversification of regulatory and proinflammatory chromatin states in TH17 cells. Nature Immunology 2024, 25: 1395-1410. PMID: 39009838, DOI: 10.1038/s41590-024-01901-1.Peer-Reviewed Original ResearchConceptsTransposase-accessible chromatin sequencingSingle-cell RNA sequencingTh17 heterogeneitySingle-cell assaysScATAC-seqChromatin landscapeChromatin stateChromatin sequencingRegulatory networksScRNA-seqTh17 cell pathogenicityHuman geneticsIn vivoRNA sequencingChromatin configurationRegulatory pathwaysTissue homeostasisCell statesCells in vitroBach2ChromatinSequenceCellsType 1 helper T (Th1) cellsCD4+ T cell subsetsAutoimmunity and the microbiome
Cox L, Kuchroo V. Autoimmunity and the microbiome. Immunological Reviews 2024, 325: 4-8. PMID: 38980198, DOI: 10.1111/imr.13363.Peer-Reviewed Original ResearchTIGIT predominantly regulates the immune response via regulatory T cells
Kurtulus S, Sakuishi K, Ngiow S, Joller N, Tan D, Teng M, Smyth M, Kuchroo V, Anderson A. TIGIT predominantly regulates the immune response via regulatory T cells. Journal Of Clinical Investigation 2024, 134: e183278. PMID: 38949028, PMCID: PMC11213499, DOI: 10.1172/jci183278.Peer-Reviewed Original ResearchOncogene induced TIM-3 ligand expression dictates susceptibility to anti-TIM-3 therapy in mice
Talvard-Balland N, Braun L, Dixon K, Zwick M, Engel H, Hartmann A, Duquesne S, Penter L, Andrieux G, Rindlisbacher L, Acerbis A, Ehmann J, Köllerer C, Ansuinelli M, Rettig A, Moschallski K, Apostolova P, Brummer T, Illert A, Schramm M, Cheng Y, Köttgen A, Duyster J, Menssen H, Ritz J, Blazar B, Boerries M, Graeff A, Sariipek N, van Galen P, Buescher J, Cabezas-Wallscheid N, Pahl H, Pearce E, Soiffer R, Wu C, Vago L, Becher B, Köhler N, Wertheimer T, Kuchroo V, Zeiser R. Oncogene induced TIM-3 ligand expression dictates susceptibility to anti-TIM-3 therapy in mice. Journal Of Clinical Investigation 2024, 134: e177460. PMID: 38916965, PMCID: PMC11324309, DOI: 10.1172/jci177460.Peer-Reviewed Original ResearchAcute graft-versus-host diseaseAcute myeloid leukemiaPost-allo-HCTTim-3GVL effectLigand expressionGraft-versus-host diseaseAllogeneic hematopoietic cell transplantationCD8+ T cellsEnhanced GVL effectIFN-g productionLow ligand expressionTim-3 ligandAnti-PD-1TIM-3 blockadeAnti-Tim-3Hematopoietic cell transplantationSurvival of miceCells of patientsAllo-HCTLeukemia relapseCell transplantationCause of deathMyeloid leukemiaT cellsHuman regulatory memory B cells defined by expression of TIM-1 and TIGIT are dysfunctional in multiple sclerosis
Varghese J, Kaskow B, von Glehn F, Case J, Li Z, Julé A, Berdan E, Sui S, Hu Y, Krishnan R, Chitnis T, Kuchroo V, Weiner H, Baecher-Allan C. Human regulatory memory B cells defined by expression of TIM-1 and TIGIT are dysfunctional in multiple sclerosis. Frontiers In Immunology 2024, 15: 1360219. PMID: 38745667, PMCID: PMC11091236, DOI: 10.3389/fimmu.2024.1360219.Peer-Reviewed Original ResearchConceptsMemory B cell subsetsMemory B cellsB cell subsetsT cell activationB cellsT cellsMultiple sclerosisIL-17ATIM-1Levels of CD4+ T cell activationAllogeneic CD4+ T cellsRelapsing-remittingAssociated with response to therapyAnti-CD20 treated patientsCD4+ T cell activationDouble positive (DPCD4+ T cellsExpression of TIM-1Genes associated with T-cell activationInduction of inflammatory markersInduce T cell proliferationHuman memory B cellsLack of cell surface markersRegulatory B cellsExpression of genes associated with T-cell activationIL-23 past, present, and future: a roadmap to advancing IL-23 science and therapy
Krueger J, Eyerich K, Kuchroo V, Ritchlin C, Abreu M, Elloso M, Fourie A, Fakharzadeh S, Sherlock J, Yang Y, J. D, McInnes I. IL-23 past, present, and future: a roadmap to advancing IL-23 science and therapy. Frontiers In Immunology 2024, 15: 1331217. PMID: 38686385, PMCID: PMC11056518, DOI: 10.3389/fimmu.2024.1331217.Peer-Reviewed Original ResearchConceptsImmune-mediated inflammatory diseasesIL-23 inhibitionIL-23Anti-IL-23 therapyOptimal identification of patientsTreat immune-mediated inflammatory diseasesIL-12 cytokine family memberInterleukin (IL)-23Identification of patientsIL-23 signalingCytokine family membersInflammatory bowel diseaseMolecular ontologiesRegulatory cytokinesIL-12Psoriatic arthritisInflammatory diseasesBowel diseaseDiseaseTherapyFamily membersBiologyCharting the cellular biogeography in colitis reveals fibroblast trajectories and coordinated spatial remodeling
Cadinu P, Sivanathan K, Misra A, Xu R, Mangani D, Yang E, Rone J, Tooley K, Kye Y, Bod L, Geistlinger L, Lee T, Mertens R, Ono N, Wang G, Sanmarco L, Quintana F, Anderson A, Kuchroo V, Moffitt J, Nowarski R. Charting the cellular biogeography in colitis reveals fibroblast trajectories and coordinated spatial remodeling. Cell 2024, 187: 2010-2028.e30. PMID: 38569542, PMCID: PMC11017707, DOI: 10.1016/j.cell.2024.03.013.Peer-Reviewed Original ResearchConceptsMultiplexed error-robust fluorescence in situ hybridizationFluorescence in situ hybridizationSpatial organizationCell-cell interactionsDiverse cell populationsHealthy gutMouse colitis modelExpression profilesBiogeographyGutNon-immune cellsGut inflammationSpatial remodelingInflammation-associated fibroblastsTissue neighborhoodsInflammation-induced remodelingCell populationsFibroblastsImmune cellsCellsColitis modelUlcerative colitisFibroblastic originStage progressionExpression