Instruments

The Agilent HPLC 1260 can be used independently for quantitative analysis of protein samples or qualitative purification and preparation of protein samples. Additionally when used in tandem with the Wyatt DAWN Heleos-II MALS detector and an Optilab T-rEX RI detector properties of the protein sample including molecular weight and oligimerization state.

The Agilent HPLC 1260 has a quaternary pump allowing for up to 4 mobile phases as well as gradients, the system has an auto-injector allowing numerous samples to be run sequentially without intervention. Also the system includes a UV-VIS detector, a fluorescent detector and a fraction collector.

• HPLC
• Manual
• Wyatt MALS and RI Detectors

The X-ray core laboratory has 2 HPLC columns available for use.

1. A Sepax SRT-300 for SEC separation
2. A Wyatt WTC-015S5 used exclusively for SEC-MALS experiments.

Additionally users are encouraged to use additional columns specific to their experimental design.

The Nano ITC Low Volume isothermal titration calorimeter is designed to provide maximum sensitivity and flexibility for the study of biomolecular binding. The instruments uses solid state thermoelectric heating and cooling systems to precisely control temperature, and has a flexible injection syringe assembly for efficient and accurate delivery of titrant. The true isothermal power compensation design of the Nano ITC instrument provides the highest sensitivity and flexibility for an ultrasensitive ITC analyzing biological samples in-solution.

Associated with the Nano ITC the X-ray core laboratory has a sample degasser, sample cell cleaner, dedicated computer with remote access available to monitor experiments in progress and several titrant syringes for practice. It is highly recommended that researchers provide their own titrant syringe (Part # 5346, TA instruments).

The STPLabtech Mosquito liquid handling robot is a robust, reliable tool used in the preparation of crystallization experiments. Many crystallization methods can be done efficiently including; hanging drop, multiple drop per well hanging drop, sitting drop, seeding, additive screens, under oil, multiple protein complexes, sparse matrix screens and optimization screens. In addition to crystallographic experiments the Mosquito can be utilized as an advanced liquid handling system. It can pipet into 96, 384 and 1536 well SBS format plates and has an accuracy of 20 nL.

The Mosquito has been successfully used in a variety of other applications where accurate and precise pipetting of nanoliter volumes of liquid is critical.

High-Throughput MALDI-TOF
A TTPLabtech Mosquito was used in the following publication: Heap RE, Hope AG, Pearson LA, et al. Identifying Inhibitors of Inflammation: A Novel High-Throughput MALDI-TOF Screening Assay for Salt-Inducible Kinases (SIKs). SLAS Discov. 2017;22(10):1193-1202.
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Single cell RNA Sequencing method (Cel-seq2)
A TTPLabtech Mosquito was used in the following publication: Sagar, Pokrovskii M, Herman JS, et al. Deciphering the regulatory landscape of fetal and adult ?d T-cell development at single-cell resolution. EMBO J. 2020;39(13):e104159.
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Nextera XT library preparation and magnetic bead clean up.
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Automated high-density sample storage and Miniaturized liquid handling for high-throughput synthetic biology construct assembly.
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Using Mosquito HTS for Gateway cloning, PCR amplification and Nextera library generation.
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• Information from STPLabtech can be found on their website.

STPLabtech also provides an extensive series of tutorial and usage videos on their Youtube channel.

Click here to visit the Vendor's Website.

The Formulatrix NT8 liquid handling robot is a robust, reliable tool used in the preparation of crystallization experiments. Many crystallization methods can be done efficiently including; hanging drop, multiple drop per well hanging drop, sitting drop, seeding, additive screens, under oil, multiple protein complexes, sparse matrix screens and optimization screens. In addition to crystallographic experiments the Mosquito can be utilized as an advanced liquid handling system. It can pipet into 96 and 384 SBS format plates including deep well blocks and has an accuracy of 20 nL.

NT8 YouTube Channel

NT8 also provides an extensive series of tutorial and usage videos on their Youtube channel.

The RI-1000 is a powerful tool for crystallographers providing a multitude of uses including; scheduling imaging, imaging in Visible, UV, cross-polarization and fluorescence.

Remote viewing of experimental results is done with RockmakerWeb

Check the Formulatrix website for UV compatibility of your media

The Rockimager 1 is stored in a 4°C cold room and used to image a single experimental plate at a time either in Visible light or UV illumination. Experiment plates can be stored in the cold room and the user will image them at their own imaging schedule. The RI-1 can handle any SBS format plate as well a more traditional 24-well Lindbro plate set by hand. The databases between the RI-1, RI-1000 and Rockmaker software are linked so images from 4C can be viewed remotely.

Remote viewing of experimental results is done with RockmakerWeb

The laboratory has 3 vibration-free incubators purposed to expand upon storage space in the RI-1000 and to allow crystals to be grown at varying temperatures. Currently they are set at 20°C, 12°C and 4°C. The 12°C is a ‘swap’ temperature and can be set at other temperatures at a researcher’s request.

A recent acquisition funded by an NIH S-10 shared instrumentation grant is a state of the art in-house data collection system. The 4-axis goniometer coupled with an ultra-sensitive Pixel Array Detector allows convenient, rapid, high resolution data collection within the laboratory.