1996
KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells
Cui Z, Hidaka H, Dannies P. KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells. Biochimica Et Biophysica Acta 1996, 1310: 343-347. PMID: 8599613, DOI: 10.1016/0167-4889(95)00170-0.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAnimalsCalciumCalcium-Calmodulin-Dependent Protein KinasesCells, CulturedEnzyme InhibitorsFemaleIsoquinolinesKineticsPiperazinesPituitary Gland, AnteriorPotassiumPotassium ChlorideProlactinRatsRats, Sprague-DawleyThyrotropin-Releasing HormoneTime FactorsConceptsAnterior pituitary cellsProlactin secretionCalcium/calmodulin-dependent protein kinase IIPituitary cellsCalmodulin-dependent protein kinase IIKN-04KN-62Rat anterior pituitary cellsVoltage-dependent calcium channelsProtein kinase IICalmodulin-dependent protein kinase II inhibitorKCl-stimulated increasesIntracellular calcium increaseCalcium/calmodulin-dependent protein kinase II inhibitorKinase II inhibitorCalcium channelsCalcium increaseIntracellular Ca2Kinase IISecretionInhibits activationII inhibitorsIC50InhibitorsCells
1994
Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response Gene
Holt E, Lu C, Dreyer B, Dannies P, Broadus A. Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response Gene. Journal Of Neurochemistry 1994, 62: 1239-1246. PMID: 8133258, DOI: 10.1046/j.1471-4159.1994.62041239.x.Peer-Reviewed Original ResearchConceptsGH4C1 rat pituitary cellsRat pituitary cellsPituitary cellsParathyroid hormone-related peptide gene expressionPTHrP mRNA expressionPrimary response geneParathyroid hormone-related peptide genePTHrP gene transcriptionPeptide gene expressionResponse genesPharmacological controlMRNA responseMRNA expressionTransient increaseGrowth factorGene transcriptionPlateau responsePeak response
1990
Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells
Law G, Pachter J, Thastrup O, Hanley M, Dannies P. Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells. Biochemical Journal 1990, 267: 359-364. PMID: 1692207, PMCID: PMC1131296, DOI: 10.1042/bj2670359.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCaffeineCalciumCell LineInositol PhosphatesKineticsNimodipinePituitary NeoplasmsPlants, MedicinalProlactinTerpenesThapsigarginThyrotropin-Releasing HormoneTumor Cells, CulturedConceptsThyrotropin-releasing hormoneGH4C1 pituitary cellsPituitary cellsIntracellular storesGH4C1 cellsAbility of TRHBay K 8644Cytosolic free Ca2Action of thapsigarginInsP3-sensitive storesSustained elevationChannel blockersReceptor levelsTRH stimulationExtracellular Ca2Intracellular Ca2Transient increaseFree Ca2ThapsigarginSubsequent spikesHormoneCell typesCa2CellsEffluxComparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1988
Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH
Pachter J, Law G, Dannies P. Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH. Biochemical And Biophysical Research Communications 1988, 154: 654-659. PMID: 3135808, DOI: 10.1016/0006-291x(88)90189-1.Peer-Reviewed Original Research
1986
Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary cultures
Martinez-Campos A, Amara J, Dannies P. Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary cultures. Molecular And Cellular Endocrinology 1986, 48: 127-133. PMID: 3803705, DOI: 10.1016/0303-7207(86)90035-3.Peer-Reviewed Original ResearchConceptsProlactin productionPituitary cellsMale rat anterior pituitary cellsRat anterior pituitary cellsPartial estrogen agonistNormal pituitary cellsAnterior pituitary cellsConcentrations of estradiolDose-response relationshipPrimary pituitary culturesEstrogen antagonistLY 117018Estrogen agonistM tamoxifenPartial agonistPituitary culturesEstrogen inductionTamoxifenMaximal stimulationFull antagonistM LysPrimary culturesStimulationPimozideAgonists
1985
Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures
Delbeke D, Kojima I, Dannies P. Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures. Molecular And Cellular Endocrinology 1985, 43: 15-22. PMID: 3934015, DOI: 10.1016/0303-7207(85)90037-1.Peer-Reviewed Original ResearchStimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*
DELBEKE D, DANNIES P. Stimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*. Endocrinology 1985, 117: 439-446. PMID: 2990850, DOI: 10.1210/endo-117-2-439.Peer-Reviewed Original ResearchConceptsPRL releaseDopaminergic inhibitionAbsence of dopamineMessenger systemsRat anterior pituitary cellsPattern of releaseAnterior pituitary cellsCAMP messenger systemMin of preincubationBasal releaseProlactin releaseNM TRHPituitary cellsIntracellular Ca2Sustained phasePhorbol ester 12Cytosolic Ca2Intracellular cAMPTRHAdenylate cyclaseCAMP levelsDopamineSustained releaseLevel of releaseC-kinase activity
1984
Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*
DELBEKE D, SCAMMELI J, DANNIES P. Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*. Endocrinology 1984, 114: 1433-1440. PMID: 6323146, DOI: 10.1210/endo-114-4-1433.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPRL releaseMale rat pituitary glandPrimary culturesNormal cellsNormal pituitary cellsPresence of D600Release of prolactinRat pituitary glandAddition of forskolinIntracellular cAMP concentrationPituitary tumorsFischer ratsPituitary cellsPituitary glandChannel inhibitorsTumor cellsTypes of cellsAdenylate cyclaseD600ForskolinCAMP concentrationCalcium requirementForskolin stimulationCellsSpiperone: evidence for uptake into secretory granules.
Dannies P, Rudnick M, Fishkes H, Rudnick G. Spiperone: evidence for uptake into secretory granules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1867-1870. PMID: 6584920, PMCID: PMC345023, DOI: 10.1073/pnas.81.6.1867.Peer-Reviewed Original ResearchConceptsSerotonin receptorsSecretory granulesRat pituitary tumor cellsMicroM reserpinePlatelet dense granulesPituitary tumor cellsDopamine antagonistsIntracellular storage organellesSpiperoneTumor cellsSerotonin transportDense granulesBiogenic amine transportReceptorsCell strainsDopamineEquimolar concentrationsSpecific ligandsAmine transportStorage organellesPlasma membrane transport systemsReserpineAntagonistMinNeurons
1983
17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1
AMARA J, DANNIES P. 17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1. Endocrinology 1983, 112: 1141-1143. PMID: 6822206, DOI: 10.1210/endo-112-3-1141.Peer-Reviewed Original ResearchConceptsHalf-maximal concentrationGH cellsProlactin productionEffects of estradiolPituitary tumor growthDose-dependent decreaseCell numberHigh oestrogen concentrationsRat pituitary tumor cellsPituitary tumor cellsBeta-EstradiolBiphasic effectEstrogen concentrationsTumor growthEstradiolTumor cellsInhibitory effectMaximal concentrationInhibition of growthBiphasic fashionSerumStimulationCell growthHorse serumCells
1982
Histidyl-proline diketopiperazine: its biological role as a regulatory peptide
Peterkofsky A, Battaini F, Koch Y, Takahara Y, Dannies P. Histidyl-proline diketopiperazine: its biological role as a regulatory peptide. Molecular And Cellular Biochemistry 1982, 42: 45-63. PMID: 6278283, DOI: 10.1007/bf00223538.Peer-Reviewed Original ResearchConceptsHistidyl-proline diketopiperazineCentral nervous systemAdrenal cortex membranesCyclic nucleotide levelsCNS depressionSpecific membrane receptorsCortex membranesProlactin releaseRat brainClassical neurotransmittersNervous systemPituitary cellsTRHSuch receptorsRegulatory peptidesPituitaryStereotypic behaviorImmunological methodsSpecific bindingBrainMembrane receptorsReceptorsDistribution studiesPossible mechanismNucleotide levelHormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells.
Kiino D, Dannies P. Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells. The Yale Journal Of Biology And Medicine 1982, 55: 409-20. PMID: 6763814, PMCID: PMC2596570.Peer-Reviewed Original ResearchConceptsProlactin storageRat pituitary tumor cellsGH cellsPituitary tumor cellsPituitary functionNM estradiolTumor cellsProlactin synthesisEstradiol-induced increaseCells/NM insulinDays of treatmentProlactin-secreting cellsCell cycle distributionPercent increaseClonal strainsHormone secretionEpidermal growth factorPotassium depolarizationProlactin productionEstradiolHormone productionProlactinInsulinIntracellular prolactin
1981
Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*
KIINO D, DANNIES P. Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*. Endocrinology 1981, 109: 1264-1269. PMID: 7026222, DOI: 10.1210/endo-109-4-1264.Peer-Reviewed Original ResearchConceptsIntracellular PRLEffect of insulinDays of treatmentHalf-maximal doseRat pituitary tumor cellsPituitary tumor cellsIntracellular prolactin contentM estradiolEstradiolCombined treatmentPRLInsulinPRL synthesisTumor cellsGH4C1 cellsM insulinControl levelsProlactin contentIntracellular hormoneM. TreatmentTotal proteinTreatmentDoseIntracellular transit timeSmall increaseMuscarinic inhibition of prolactin production in cultures of rat pituitary cells
Rudnick M, Dannies P. Muscarinic inhibition of prolactin production in cultures of rat pituitary cells. Biochemical And Biophysical Research Communications 1981, 101: 689-696. PMID: 7306103, DOI: 10.1016/0006-291x(81)91313-9.Peer-Reviewed Original Research
1980
Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells.
Tam S, Dannies P. Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells. Journal Of Biological Chemistry 1980, 255: 6595-6599. PMID: 6771274, DOI: 10.1016/s0021-9258(18)43611-3.Peer-Reviewed Original ResearchAction of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*
DANNIES P, TASHJIAN A. Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*. Endocrinology 1980, 106: 1532-1536. PMID: 6244936, DOI: 10.1210/endo-106-5-1532.Peer-Reviewed Original ResearchConceptsGH synthesisCholera toxinIntracellular cAMPWeeks of treatmentThyrotropin-releasing hormoneRelease of PRLHormone synthesisRat pituitary cellsIntracellular PRLGrowth hormone synthesisPRL releaseSimilar lag periodPituitary cellsGH cellsControl valuesPRL synthesisTRHPRLToxinClonal strainsLag periodReleaseCellsCAMPDecreaseEffects of Estradiol on Prolactin Production and Dihydroergocryptine-Induced Inhibition of Prolactin Production in Primary Cultures of Rat Pituitary Cells*
WEST B, DANNIES P. Effects of Estradiol on Prolactin Production and Dihydroergocryptine-Induced Inhibition of Prolactin Production in Primary Cultures of Rat Pituitary Cells*. Endocrinology 1980, 106: 1108-1113. PMID: 6766855, DOI: 10.1210/endo-106-4-1108.Peer-Reviewed Original Research2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment.
Dannies P, Rudnick M. 2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment. Journal Of Biological Chemistry 1980, 255: 2776-2781. PMID: 7358708, DOI: 10.1016/s0021-9258(19)85806-4.Peer-Reviewed Original ResearchDifferential Ability of Thyrotropin-Releasing Hormone and N3im-Methyl-Thyrotropin-Releasing Hormone to Affect Prolactin and Thyrotropin Production in Primary Rat Pituitary Cell Cultures*
DANNIES P, MARKELL M. Differential Ability of Thyrotropin-Releasing Hormone and N3im-Methyl-Thyrotropin-Releasing Hormone to Affect Prolactin and Thyrotropin Production in Primary Rat Pituitary Cell Cultures*. Endocrinology 1980, 106: 107-112. PMID: 6765928, DOI: 10.1210/endo-106-1-107.Peer-Reviewed Original Research