2000
Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor
Lee M, Zhu Y, Sun Z, Rhee H, Jeromin A, Roder J, Dannies P. Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor. Endocrinology 2000, 141: 3485-3492. PMID: 10965922, DOI: 10.1210/endo.141.9.7647.Peer-Reviewed Original ResearchConceptsSecretory pathwayEpidermal growth factorSynaptotagmin IIICyclophilin BGH4C1 cellsInduction of proteinsGrowth factorGlucose-regulated protein 94Rat pituitary GH4C1 cellsSecretory granulesSynaptosomal-associated proteinMessenger RNA accumulationMembrane proteinsPituitary GH4C1 cellsSynaptosomal associated proteinRNA accumulationPlasma membraneProtein AssociatedSpecific proteinsSynaptotagmin IHormone-treated cellsSNAP-25Protein 94Secretory granule accumulationCoordinate event
1999
Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting*
Dannies P. Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting*. Endocrine Reviews 1999, 20: 3-21. PMID: 10047971, DOI: 10.1210/edrv.20.1.0354.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCytoplasmic GranulesEndoplasmic ReticulumGolgi ApparatusHormonesHumansHydrolasesLysosomesNeurosecretory SystemsProteinsConceptsTrans-Golgi networkSecretory granule proteinsMembrane proteinsGranule proteinsSecretory granule membrane proteinsPossible recognition siteGranule membrane proteinSecretory granule membranesThree-dimensional electron microscopyTransport vesiclesCell biologyConstitutive pathwayRegulated pathwayMembrane lipidsGranule formationGranule membranesSorting mechanismHormone aggregationProteinDense core granulesProcess of aggregationRecognition sitesSame cellsNeuroendocrine cellsSecretory granulesProtein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting
Dannies P. Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting. Endocrine Reviews 1999, 20: 3-21. DOI: 10.1210/er.20.1.3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCytoplasmic GranulesEndoplasmic ReticulumGolgi ApparatusHormonesHumansHydrolasesLysosomesNeurosecretory SystemsProteinsConceptsTrans-Golgi networkSecretory granule proteinsMembrane proteinsGranule proteinsSecretory granule membrane proteinsPossible recognition siteGranule membrane proteinSecretory granule membranesThree-dimensional electron microscopyTransport vesiclesCell biologyConstitutive pathwayRegulated pathwayMembrane lipidsGranule formationGranule membranesSorting mechanismHormone aggregationProteinDense core granulesProcess of aggregationRecognition sitesSame cellsNeuroendocrine cellsSecretory granules
1998
Stabilization of the receptor protein tyrosine phosphatase-like protein ICA512 in GH4C1 cells upon treatment with estradiol, insulin, and epidermal growth factor.
Lee M, Dirkx R, Solimena M, Dannies P. Stabilization of the receptor protein tyrosine phosphatase-like protein ICA512 in GH4C1 cells upon treatment with estradiol, insulin, and epidermal growth factor. Endocrinology 1998, 139: 2727-33. PMID: 9607778, DOI: 10.1210/endo.139.6.6039.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantigensCytoplasmic GranulesDrug StabilityEpidermal Growth FactorEstradiolImmunoblottingImmunologic TechniquesInsulinMembrane ProteinsMicroscopy, ConfocalPeptide FragmentsPrecipitin TestsProtein PrecursorsProtein Tyrosine PhosphatasesRatsReceptor-Like Protein Tyrosine Phosphatases, Class 8Time FactorsTumor Cells, Cultured
1994
Inhibition of rat prolactin (PRL) storage by coexpression of human PRL.
Arrandale J, Dannies P. Inhibition of rat prolactin (PRL) storage by coexpression of human PRL. Endocrinology 1994, 8: 1083-1090. PMID: 7997234, DOI: 10.1210/mend.8.8.7997234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAsparagineBiological TransportCell CompartmentationCytoplasmic GranulesEpidermal Growth FactorEstradiolGene ExpressionHumansInsulinModels, MolecularPituitary NeoplasmsProlactinProtein ConformationRatsRecombinant Fusion ProteinsSerineSpecies SpecificityTransfectionTumor Cells, CulturedConceptsHuman PRLRat PRLGH4C1 cellsReceptor-mediated mechanismNM epidermal growth factorSecretory granulesNM estradiolDense-core secretory granulesEpidermal growth factorHormone treatmentPRL storageProlactin storagePRLGrowth factorNM insulinMore ratsUntransfected cellsRatsRegulated pathwayControl culturesCellsInhibitionMarked specificity
1991
Is a sorting signal necessary to package proteins into secretory granules?
Reaves B, Dannies P. Is a sorting signal necessary to package proteins into secretory granules? Molecular And Cellular Endocrinology 1991, 79: c141-c145. PMID: 1936531, DOI: 10.1016/0303-7207(91)90085-7.Peer-Reviewed Original Research
1990
Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1986
Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*
SCAMMELL J, BURRAGE T, DANNIES P. Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*. Endocrinology 1986, 119: 1543-1548. PMID: 3530721, DOI: 10.1210/endo-119-4-1543.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCytoplasmic GranulesEpidermal Growth FactorEstradiolInsulinMicroscopy, ElectronPituitary NeoplasmsProlactinRatsConceptsGH4C1 cellsIntracellular PRLSecretory granulesCombination of estradiolNumber of granulesPituitary tumor cell linePRL accumulationHormone regimenTumor cell strainsEpidermal growth factorHormone treatmentTumor cell linesPRLGrowth factorControl levelsOccasional granulesCell linesHormonal inductionGHCell strainsCellsGranule numberTreatmentCellular contentStorage granules
1985
Cysteamine Causes Reduction of Prolactin Monomers Followed by Aggregation in the Rat Pituitary Gland*
SCAMMELL J, BURRAGE T, EISENFELD A, DANNIES P. Cysteamine Causes Reduction of Prolactin Monomers Followed by Aggregation in the Rat Pituitary Gland*. Endocrinology 1985, 116: 2347-2354. PMID: 3996317, DOI: 10.1210/endo-116-6-2347.Peer-Reviewed Original Research
1984
Spiperone: evidence for uptake into secretory granules.
Dannies P, Rudnick M, Fishkes H, Rudnick G. Spiperone: evidence for uptake into secretory granules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1867-1870. PMID: 6584920, PMCID: PMC345023, DOI: 10.1073/pnas.81.6.1867.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportButyrophenonesCells, CulturedCytoplasmic GranulesKineticsPituitary GlandRatsSpiperoneConceptsSerotonin receptorsSecretory granulesRat pituitary tumor cellsMicroM reserpinePlatelet dense granulesPituitary tumor cellsDopamine antagonistsIntracellular storage organellesSpiperoneTumor cellsSerotonin transportDense granulesBiogenic amine transportReceptorsCell strainsDopamineEquimolar concentrationsSpecific ligandsAmine transportStorage organellesPlasma membrane transport systemsReserpineAntagonistMinNeurons