2002
Prolonged Retention after Aggregation into Secretory Granules of Human R183H-Growth Hormone (GH), a Mutant that Causes Autosomal Dominant GH Deficiency Type II
Zhu YL, Conway-Campbell B, Waters MJ, Dannies PS. Prolonged Retention after Aggregation into Secretory Granules of Human R183H-Growth Hormone (GH), a Mutant that Causes Autosomal Dominant GH Deficiency Type II. Endocrinology 2002, 143: 4243-4248. PMID: 12399418, DOI: 10.1210/en.2002-220575.Peer-Reviewed Original Research
2000
Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor
Lee M, Zhu Y, Sun Z, Rhee H, Jeromin A, Roder J, Dannies P. Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor. Endocrinology 2000, 141: 3485-3492. PMID: 10965922, DOI: 10.1210/endo.141.9.7647.Peer-Reviewed Original ResearchConceptsSecretory pathwayEpidermal growth factorSynaptotagmin IIICyclophilin BGH4C1 cellsInduction of proteinsGrowth factorGlucose-regulated protein 94Rat pituitary GH4C1 cellsSecretory granulesSynaptosomal-associated proteinMessenger RNA accumulationMembrane proteinsPituitary GH4C1 cellsSynaptosomal associated proteinRNA accumulationPlasma membraneProtein AssociatedSpecific proteinsSynaptotagmin IHormone-treated cellsSNAP-25Protein 94Secretory granule accumulationCoordinate eventAutosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH
Lee M, Wajnrajch M, Kim S, Plotnick L, Wang J, Gertner J, Leibel R, Dannies P. Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH. Endocrinology 2000, 141: 883-890. PMID: 10698162, DOI: 10.1210/endo.141.3.7380.Peer-Reviewed Original ResearchConceptsWild-type GHSecretory pathway functionNeuroendocrine cell lineGH deficiency type IISuppression of accumulationPathway functionTransient transfectionIntracellular stabilityCHO cellsAutosomal dominant formCell linesDecreased stabilityNormal allelePosttranslational effectGeneral suppressionCoexpressionProtein
1997
Editorial: A New Releasing Factor? with Biotechnology and a Little Bit of Luck
Dannies P. Editorial: A New Releasing Factor? with Biotechnology and a Little Bit of Luck. Endocrinology 1997, 138: 5085-5086. PMID: 9389485, DOI: 10.1210/endo.138.12.5710.Peer-Reviewed Original ResearchInefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+.
Sun Z, Lee M, Rhee H, Arrandale J, Dannies P. Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+. Endocrinology 1997, 11: 1544-51. PMID: 9280069, DOI: 10.1210/mend.11.10.0002.Peer-Reviewed Original Research
1996
Properties of human prolactin (PRL) and H27A-PRL, a mutant that does not bind Zn++.
Sun Z, Li P, Dannies P, Lee J. Properties of human prolactin (PRL) and H27A-PRL, a mutant that does not bind Zn++. Endocrinology 1996, 10: 265-71. PMID: 8833655, DOI: 10.1210/mend.10.3.8833655.Peer-Reviewed Original Research
1995
Biological activity and immunological reactivity of human prolactin mutants
Rhee H, Sun Z, Kim S, Goffin V, Martial J, Dannies P. Biological activity and immunological reactivity of human prolactin mutants. Endocrinology 1995, 136: 4990-4995. PMID: 7588233, DOI: 10.1210/endo.136.11.7588233.Peer-Reviewed Original ResearchConceptsSerine 90Cystine loopBiological activityRat pituitary cell lineHuman PRLPituitary cell lineWild typeCertain fishPRL-3MutantsTryptophan fluorescenceCell linesMutationsFishT mutationCell assayPRL storageImmunological reactivityImmunological propertiesSerineConcentration of ureaActivityConservationSpacial requirementsLoop
1994
Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response Gene
Holt E, Lu C, Dreyer B, Dannies P, Broadus A. Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response Gene. Journal Of Neurochemistry 1994, 62: 1239-1246. PMID: 8133258, DOI: 10.1046/j.1471-4159.1994.62041239.x.Peer-Reviewed Original ResearchConceptsGH4C1 rat pituitary cellsRat pituitary cellsPituitary cellsParathyroid hormone-related peptide gene expressionPTHrP mRNA expressionPrimary response geneParathyroid hormone-related peptide genePTHrP gene transcriptionPeptide gene expressionResponse genesPharmacological controlMRNA responseMRNA expressionTransient increaseGrowth factorGene transcriptionPlateau responsePeak response
1990
Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells
Law G, Pachter J, Thastrup O, Hanley M, Dannies P. Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells. Biochemical Journal 1990, 267: 359-364. PMID: 1692207, PMCID: PMC1131296, DOI: 10.1042/bj2670359.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCaffeineCalciumCell LineInositol PhosphatesKineticsNimodipinePituitary NeoplasmsPlants, MedicinalProlactinTerpenesThapsigarginThyrotropin-Releasing HormoneTumor Cells, CulturedConceptsThyrotropin-releasing hormoneGH4C1 pituitary cellsPituitary cellsIntracellular storesGH4C1 cellsAbility of TRHBay K 8644Cytosolic free Ca2Action of thapsigarginInsP3-sensitive storesSustained elevationChannel blockersReceptor levelsTRH stimulationExtracellular Ca2Intracellular Ca2Transient increaseFree Ca2ThapsigarginSubsequent spikesHormoneCell typesCa2CellsEfflux
1988
Expression of messenger ribonucleic acids encoding a parathyroid hormone-like peptide in normal human and animal tissues with abnormal expression in human parathyroid adenomas.
Ikeda K, Weir E, Mangin M, Dannies P, Kinder B, Deftos L, Brown E, Broadus A. Expression of messenger ribonucleic acids encoding a parathyroid hormone-like peptide in normal human and animal tissues with abnormal expression in human parathyroid adenomas. Endocrinology 1988, 2: 1230-6. PMID: 3216862, DOI: 10.1210/mend-2-12-1230.Peer-Reviewed Original ResearchConceptsPTH-like peptideHuman parathyroid adenomasParathyroid adenomaAbnormal human parathyroid tissueHuman parathyroid tissueNumber of endocrineMedullary carcinoma cellsParathyroid hormone-like peptideExpression of mRNAAutonomous glandsHumoral hypercalcemiaParathyroid tissueHormone-like peptideAdrenal cortexRat brainNonendocrine tissuesAdrenal medullaBone marrowMessenger ribonucleic acidStomach mucosaHuman osteosarcomaOverexpression of transcriptsRat pituitaryNormal humansAbnormal expressionBombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH
Pachter J, Law G, Dannies P. Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH. Biochemical And Biophysical Research Communications 1988, 154: 654-659. PMID: 3135808, DOI: 10.1016/0006-291x(88)90189-1.Peer-Reviewed Original Research
1987
Regulation of Prolactin Production and Cell Growth by Estradiol: Difference in Sensitivity to Estradiol Occurs at Level of Messenger Ribonucleic Acid Accumulation*
AMARA J, VAN ITALLIE C, DANNIES P. Regulation of Prolactin Production and Cell Growth by Estradiol: Difference in Sensitivity to Estradiol Occurs at Level of Messenger Ribonucleic Acid Accumulation*. Endocrinology 1987, 120: 264-271. PMID: 3780563, DOI: 10.1210/endo-120-1-264.Peer-Reviewed Original ResearchConceptsPRL productionMaximal stimulationGH4C1 cellsOrder of potencyMessenger ribonucleic acid accumulationBiological effectsBeta-EstradiolCell growthEstrogen concentrationsProlactin productionEthynyl estradiolFull agonistSame receptorEstradiolStimulationEstrogenPRL mRNAEffective concentrationAnalog potencyPotencyCellsRibonucleic acid accumulationMRNA
1986
Characterization of antiestrogen stimulation of cell number and prolactin production
Amara J, Dannies P. Characterization of antiestrogen stimulation of cell number and prolactin production. Molecular And Cellular Endocrinology 1986, 47: 183-189. PMID: 3758472, DOI: 10.1016/0303-7207(86)90111-5.Peer-Reviewed Original ResearchHormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*
SCAMMELL J, BURRAGE T, DANNIES P. Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*. Endocrinology 1986, 119: 1543-1548. PMID: 3530721, DOI: 10.1210/endo-119-4-1543.Peer-Reviewed Original ResearchConceptsGH4C1 cellsIntracellular PRLSecretory granulesCombination of estradiolNumber of granulesPituitary tumor cell linePRL accumulationHormone regimenTumor cell strainsEpidermal growth factorHormone treatmentTumor cell linesPRLGrowth factorControl levelsOccasional granulesCell linesHormonal inductionGHCell strainsCellsGranule numberTreatmentCellular contentStorage granulesRat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site.
Strobl J, Dannies P, Thompson E. Rat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site. Biochemistry 1986, 25: 3640-8. PMID: 3013292, DOI: 10.1021/bi00360a025.Peer-Reviewed Original ResearchConceptsTranscription initiation siteInitiation siteGene expressionRat growth hormone gene expressionMethylation-sensitive restriction enzymesRat growth hormone geneHybrid cellsGene control regionsGrowth hormone geneThai sitesGrowth hormone gene expressionGH3 cellsCGCG sequenceControl regionDNA methylationBp 5Gene regionGH geneHormone geneHormone gene expressionRestriction enzymesMethylation statusRestriction sitesMouse fibroblastsGH gene expression
1985
Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures
Delbeke D, Kojima I, Dannies P. Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures. Molecular And Cellular Endocrinology 1985, 43: 15-22. PMID: 3934015, DOI: 10.1016/0303-7207(85)90037-1.Peer-Reviewed Original Research
1984
Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin
Delbeke D, Kojima I, Dannies P, Rasmussen H. Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin. Biochemical And Biophysical Research Communications 1984, 123: 735-741. PMID: 6091639, DOI: 10.1016/0006-291x(84)90291-2.Peer-Reviewed Original ResearchDifference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*
DELBEKE D, SCAMMELI J, DANNIES P. Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*. Endocrinology 1984, 114: 1433-1440. PMID: 6323146, DOI: 10.1210/endo-114-4-1433.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPRL releaseMale rat pituitary glandPrimary culturesNormal cellsNormal pituitary cellsPresence of D600Release of prolactinRat pituitary glandAddition of forskolinIntracellular cAMP concentrationPituitary tumorsFischer ratsPituitary cellsPituitary glandChannel inhibitorsTumor cellsTypes of cellsAdenylate cyclaseD600ForskolinCAMP concentrationCalcium requirementForskolin stimulationCells
1983
Tamoxifen and 5-fluorouracil in breast cancer: cytotoxic synergism in vitro.
Benz C, Cadman E, Gwin J, Wu T, Amara J, Eisenfeld A, Dannies P. Tamoxifen and 5-fluorouracil in breast cancer: cytotoxic synergism in vitro. Cancer Research 1983, 43: 5298-303. PMID: 6616464.Peer-Reviewed Original ResearchConceptsHormone-dependent human breast cancer cell linesCytotoxic interactionNuclear estrogen receptor levelsEstrogen receptor levelsHuman breast cancer cell linesBreast cancer cell linesMCF-7Nuclear estrogen receptorsMCF-7 cytotoxicityCytosol progesterone receptorGrowth inhibitionCytosol progesteroneCancer cell linesMCF-7 cellsMicroM tamoxifenProgesterone receptorBreast cancerReceptor levelsEstrogen receptorNM estradiolTamoxifenIsobologram analysisClonogenic assayControl valuesCell lines17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1
AMARA J, DANNIES P. 17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1. Endocrinology 1983, 112: 1141-1143. PMID: 6822206, DOI: 10.1210/endo-112-3-1141.Peer-Reviewed Original ResearchConceptsHalf-maximal concentrationGH cellsProlactin productionEffects of estradiolPituitary tumor growthDose-dependent decreaseCell numberHigh oestrogen concentrationsRat pituitary tumor cellsPituitary tumor cellsBeta-EstradiolBiphasic effectEstrogen concentrationsTumor growthEstradiolTumor cellsInhibitory effectMaximal concentrationInhibition of growthBiphasic fashionSerumStimulationCell growthHorse serumCells