1995
Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage
Mastro R, Dannies P. Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage. Endocrinology 1995, 136: 69-74. PMID: 7828559, DOI: 10.1210/endo.136.1.7828559.Peer-Reviewed Original ResearchConceptsEpidermal growth factorNormal lactotrophsGH4C1 cellsGrowth factorForms of PRLFemale rat pituitary glandsNM epidermal growth factorRat pituitary glandDense core granulesRat pituitary tumor cellsPRL storagePituitary tumor cellsNM estradiolTotal PRLPituitary glandPRLTumor cellsCore granulesInsulinNM insulinLack of correlationLactotrophs
1990
Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated.
Reaves B, Van Itallie C, Moore H, Dannies P. Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated. Endocrinology 1990, 4: 1017-26. PMID: 2284004, DOI: 10.1210/mend-4-7-1017.Peer-Reviewed Original ResearchConceptsGH4C1 cellsEpidermal growth factorPRL synthesisGrowth factorPreferential increaseCombination of estradiolRegulated pathwayRat pituitary tumor cellsIntracellular PRLPituitary tumor cellsBasal releasePRL releaseIntracellular proinsulinSame time courseHormone treatmentPRL storageTumor cellsProinsulin immunoreactivityMRNA levelsPRLInsulinSame peak heightHormoneProinsulinTreatmentComparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1982
Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells.
Kiino D, Dannies P. Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells. The Yale Journal Of Biology And Medicine 1982, 55: 409-20. PMID: 6763814, PMCID: PMC2596570.Peer-Reviewed Original ResearchConceptsProlactin storageRat pituitary tumor cellsGH cellsPituitary tumor cellsPituitary functionNM estradiolTumor cellsProlactin synthesisEstradiol-induced increaseCells/NM insulinDays of treatmentProlactin-secreting cellsCell cycle distributionPercent increaseClonal strainsHormone secretionEpidermal growth factorPotassium depolarizationProlactin productionEstradiolHormone productionProlactinInsulinIntracellular prolactin
1981
Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*
KIINO D, DANNIES P. Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*. Endocrinology 1981, 109: 1264-1269. PMID: 7026222, DOI: 10.1210/endo-109-4-1264.Peer-Reviewed Original ResearchConceptsIntracellular PRLEffect of insulinDays of treatmentHalf-maximal doseRat pituitary tumor cellsPituitary tumor cellsIntracellular prolactin contentM estradiolEstradiolCombined treatmentPRLInsulinPRL synthesisTumor cellsGH4C1 cellsM insulinControl levelsProlactin contentIntracellular hormoneM. TreatmentTotal proteinTreatmentDoseIntracellular transit timeSmall increase