2021
Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
Marin-Lopez A, Jiang J, Wang Y, Cao Y, MacNeil T, Hastings AK, Fikrig E. Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. PLOS Neglected Tropical Diseases 2021, 15: e0009442. PMID: 34115766, PMCID: PMC8195420, DOI: 10.1371/journal.pntd.0009442.Peer-Reviewed Original ResearchConceptsSalivary gland proteinsSuccessful viral transmissionNew mammalian hostDengue virusWild habitatsHabitat expansionGland proteinsA. aegypti vectorMammalian hostsUbiquitous expressionDENV infectionGene expressionMosquito midgutProtein componentsATPase proteinVector proteinProteinSalivary glandsBlood mealViral cycleAedes aegypti mosquitoesSusceptible hostsAedes aegyptiMosquitoesHost
2011
A Tick Mannose-Binding Lectin Inhibitor Interferes with the Vertebrate Complement Cascade to Enhance Transmission of the Lyme Disease Agent
Schuijt TJ, Coumou J, Narasimhan S, Dai J, DePonte K, Wouters D, Brouwer M, Oei A, Roelofs JJ, van Dam AP, van der Poll T, Veer C, Hovius JW, Fikrig E. A Tick Mannose-Binding Lectin Inhibitor Interferes with the Vertebrate Complement Cascade to Enhance Transmission of the Lyme Disease Agent. Cell Host & Microbe 2011, 10: 136-146. PMID: 21843870, PMCID: PMC3170916, DOI: 10.1016/j.chom.2011.06.010.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBorrelia burgdorferiCell Migration AssaysCloning, MolecularComplement Membrane Attack ComplexComplement Pathway, Mannose-Binding LectinFemaleGene SilencingHemolysisHumansImmunization, PassiveImmunotherapy, ActiveInsect ProteinsIxodesLarvaLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataNeutrophilsNymphPhagocytosisRabbitsRecombinant ProteinsSalivaSalivary Proteins and PeptidesSequence AlignmentConceptsComplement cascadeLyme disease agent Borrelia burgdorferiImpaired neutrophil phagocytosisTick salivary proteinsVector-borne pathogensLyme disease agentMammalian infectionVector colonizationVertebrate hostsTick midgutAlternative complement pathwayBorrelia transmissionComplement-mediated killingVector proteinNeutrophil phagocytosisEssential rolePathway inhibitorComplement pathwayDisease agentsSalivary proteinsBorrelia burgdorferiLectin inhibitorsProteinCascadeIxodes ticks
2004
TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi
Pal U, Li X, Wang T, Montgomery RR, Ramamoorthi N, deSilva AM, Bao F, Yang X, Pypaert M, Pradhan D, Kantor FS, Telford S, Anderson JF, Fikrig E. TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi. Cell 2004, 119: 457-468. PMID: 15537536, DOI: 10.1016/j.cell.2004.10.027.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceBorrelia burgdorferiCloning, MolecularGene Expression RegulationHost-Parasite InteractionsIntestinesIxodesLipoproteinsMiceMice, Inbred C3HMolecular Sequence DataReceptors, Cell SurfaceRecombinant ProteinsConceptsLyme disease agent Borrelia burgdorferiSurvival of spirochetesTick receptorMammalian hostsRNA interferenceEfficient colonizationTROSPAOuter surface protein ABorrelia burgdorferiPathogen adherencePathogen transmissionProtein AB. burgdorferi outer surface protein AMRNA levelsIxodes scapularisB. burgdorferiColonizationSurface protein AReceptorsArthropodsBurgdorferiMammalsRepressionSpirochetesTicks
2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularis
2001
Salp25D, an Ixodes scapularis Antioxidant, Is 1 of 14 Immunodominant Antigens in Engorged Tick Salivary Glands
Das S, Banerjee G, DePonte K, Marcantonio N, Kantor F, Fikrig E. Salp25D, an Ixodes scapularis Antioxidant, Is 1 of 14 Immunodominant Antigens in Engorged Tick Salivary Glands. The Journal Of Infectious Diseases 2001, 184: 1056-1064. PMID: 11574922, DOI: 10.1086/323351.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAntigensAntioxidantsCattleCloning, MolecularConsensus SequenceEnzyme-Linked Immunosorbent AssayFemaleGene LibraryGlutathione PeroxidaseGuinea PigsHumansImmunoglobulin GInsect ProteinsIxodesMiceMice, Inbred C3HMolecular Sequence DataNematodaRabbitsRNA, MessengerSalivary GlandsSequence Homology, Amino AcidSpecies SpecificityTime Factors
1998
Cloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response
Ijdo J, Sun W, Zhang Y, Magnarelli L, Fikrig E. Cloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response. Infection And Immunity 1998, 66: 3264-3269. PMID: 9632594, PMCID: PMC108341, DOI: 10.1128/iai.66.7.3264-3269.1998.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisAgent of HGESera of patientsBorrelia burgdorferi infectionMsp-2 genesElicit specific antibodiesHumoral responseBurgdorferi infectionMurine infectionHealthy individualsPatientsSerumSpecific antibodiesHL-60 cellsInfectionAntibodiesEhrlichia chaffeensisAntigenMiceEhrlichiosisGenomic DNA expression libraryMultigene familyGlutathione transferase fusion proteinFusion protein
1995
A 55-kilodalton antigen encoded by a gene on a Borrelia burgdorferi 49-kilobase plasmid is recognized by antibodies in sera from patients with Lyme disease
Feng S, Das S, Lam T, Flavell RA, Fikrig E. A 55-kilodalton antigen encoded by a gene on a Borrelia burgdorferi 49-kilobase plasmid is recognized by antibodies in sera from patients with Lyme disease. Infection And Immunity 1995, 63: 3459-3466. PMID: 7642278, PMCID: PMC173477, DOI: 10.1128/iai.63.9.3459-3466.1995.Peer-Reviewed Original ResearchConceptsLate-stage Lyme diseaseEarly stage Lyme diseaseLyme diseaseIgM antibodiesSubset of patientsBorrelia burgdorferiLyme disease patientsEnzyme-linked immunosorbent assayRecombinant S1Disease patientsIgG antibodiesPulsed-field gel electrophoresisS1 antigenPatientsAntigenDiseaseSerum samplesImmunosorbent assaySerumAntibodiesB. burgdorferiRabbit antiBurgdorferiCultured spirochetesArthritisThe hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease.
Jwang B, Dewing P, Fikrig E, Flavell RA. The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease. MSphere 1995, 2: 609-15. PMID: 8548542, PMCID: PMC170207, DOI: 10.1128/cdli.2.5.609-615.1995.Peer-Reviewed Original ResearchConceptsFlgE proteinFlgE geneEscherichia coli expression plasmidPrimary protein sequenceB. burgdorferi N40Hook geneWhole cell lysatesHook proteinLinear chromosomesPeriplasmic flagellaProtein sequencesFlgERecombinant proteinsB. burgdorferiBasal bodiesFlagellar filamentsWestern blot analysisBorrelia burgdorferiGenesExpression plasmidProteinBlot analysisNegative bacteriaMurine antiseraLyme diseaseSelection of variant Borrelia burgdorferi isolates from mice immunized with outer surface protein A or B
Fikrig E, Tao H, Barthold SW, Flavell RA. Selection of variant Borrelia burgdorferi isolates from mice immunized with outer surface protein A or B. Infection And Immunity 1995, 63: 1658-1662. PMID: 7729870, PMCID: PMC173206, DOI: 10.1128/iai.63.5.1658-1662.1995.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupCloning, MolecularFemaleGenes, BacterialGenetic VariationImmunoblottingLipoproteinsLyme DiseaseMiceMice, Inbred C3HRecombinant ProteinsSelection, GeneticSequence Analysis, DNAVaccinationConceptsSurface protein AOuter surface protein AProtective immunityProtective antibodiesIncomplete efficacyMiceMonoclonal antibodiesProtein ABorrelia burgdorferiB. burgdorferiAntigenic diversityOspBOspAVivo selective pressureImmunityAntibodiesB. burgdorferi isolatesBurgdorferiChallenge inoculationPoint mutationsMolecular basisVaccineInfection
1994
A chromosomal Borrelia burgdorferi gene encodes a 22-kilodalton lipoprotein, P22, that is serologically recognized in Lyme disease
Lam TT, Nguyen TP, Fikrig E, Flavell RA. A chromosomal Borrelia burgdorferi gene encodes a 22-kilodalton lipoprotein, P22, that is serologically recognized in Lyme disease. Journal Of Clinical Microbiology 1994, 32: 876-883. PMID: 8027338, PMCID: PMC263156, DOI: 10.1128/jcm.32.4.876-883.1994.Peer-Reviewed Original ResearchConceptsLate-stage diseaseLyme diseaseOuter surface proteinsEarly-stage diseaseExamination of seraErythema migransSurface proteinsPulsed-field gel electrophoresisPatientsEtiologic agentDiseaseBorrelia burgdorferiB. burgdorferiAntibodiesLipoproteinBorrelia burgdorferi genesSerumBurgdorferiShort amino terminusOuter surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease
Lam TT, Nguyen TP, Montgomery RR, Kantor FS, Fikrig E, Flavell RA. Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease. Infection And Immunity 1994, 62: 290-298. PMID: 8262642, PMCID: PMC186099, DOI: 10.1128/iai.62.1.290-298.1994.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceBlotting, WesternBorrelia burgdorferi GroupCloning, MolecularCodonFluorescent Antibody TechniqueGenes, BacterialHumansLipoproteinsLyme DiseaseMolecular Sequence DataMolecular WeightOperonRegulatory Sequences, Nucleic AcidRestriction MappingSequence AlignmentSequence Homology, Nucleic AcidSolubilityConceptsOspE genesMolecular massSignal peptidase IIConsensus cleavage sequenceTranscriptional unitsLeader sequenceCommon promoterBp downstreamOuter surface proteinsProtein EStop codonSurface lipoproteinsLabeling showBorrelia burgdorferiGenesHydrophobic domainCleavage sequenceSurface proteinsAmino acidsPeptidase IIProteinOuter surface protein EGel electrophoresisNucleotidesImmunofluorescence studies
1993
Evasion of protective immunity by Borrelia burgdorferi by truncation of outer surface protein B.
Fikrig E, Tao H, Kantor FS, Barthold SW, Flavell RA. Evasion of protective immunity by Borrelia burgdorferi by truncation of outer surface protein B. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 4092-4096. PMID: 7683420, PMCID: PMC46452, DOI: 10.1073/pnas.90.9.4092.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesAntibodies, MonoclonalAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBase SequenceBorrelia burgdorferi GroupCloning, MolecularCodonEpitopesEscherichia coliFemaleFluorescent Antibody TechniqueGenes, BacterialHumansLyme DiseaseMiceMice, Inbred C3HPolymerase Chain ReactionRecombinant Fusion ProteinsRecombinant ProteinsRestriction MappingTicksVaccines, SyntheticViral VaccinesConceptsProtective immune responseVaccination immunityProtective immunityProtective antibodiesAntibody responseImmune destructionImmune responseHost defenseOuter surface proteinsLyme diseaseOuter surface protein BBorrelia burgdorferiCausative agentOspBMicePolyclonal antibodiesImmunityAntibodiesPresent studyStrain B31Surface proteinsProtein BPrevious studiesVaccinationOspB.
1990
Protection of Mice Against the Lyme Disease Agent by Immunizing with Recombinant OspA
Fikrig E, Barthold S, Kantor F, Flavell R. Protection of Mice Against the Lyme Disease Agent by Immunizing with Recombinant OspA. Science 1990, 250: 553-556. PMID: 2237407, DOI: 10.1126/science.2237407.Peer-Reviewed Original ResearchConceptsRecombinant OspALyme borreliosisC3H/HeJ miceProtection of miceTick-borne illnessB. burgdorferi strain N40Recombinant OspA proteinSurface protein AOuter surface protein AHeJ miceLyme disease agentStrain N40Borrelia burgdorferiB. burgdorferiMiceOspABorreliosisOspA proteinBurgdorferiDisease agentsProtein AVaccineIllnessAntibodies