2005
Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats.
Magnarelli LA, Bushmich SL, IJdo JW, Fikrig E. Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats. American Journal Of Veterinary Research 2005, 66: 1895-9. PMID: 16334946, DOI: 10.2460/ajvr.2005.66.1895.Peer-Reviewed Original ResearchConceptsRecombinant antigensSerum antibodiesB. burgdorferiHealthy catsBorrelia burgdorferiSeroprevalence of antibodiesWestern blot testingAnaplasma phagocytophilumA. phagocytophilumB. burgdorferi antigensWhole cell antigenA. phagocytophilum infectionIndirect fluorescent antibody stainingWestern blot analysisIxodes scapularis ticksSeropositivity rateWhole-cell B. burgdorferiSerum reactivityIll catsFluorescent antibody stainingClinical relevanceSera reactiveSpecific recombinant antigensMost catsOuter surface proteins
2001
Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Oliver JH, Fikrig E. Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis. American Journal Of Veterinary Research 2001, 62: 1365-9. PMID: 11560261, DOI: 10.2460/ajvr.2001.62.1365.Peer-Reviewed Original ResearchConceptsIgG antibodiesSerum samplesHGE agentGranulocytic ehrlichiosisClient-owned dogsDog serum samplesHuman granulocytic ehrlichiosis (HGE) agentEnzyme-linked immunosorbentPositive resultsWestern blot analysisTick-infested areasEhrlichia phagocytophila genogroupTotal immunoglobulinHorse serum samplesPromyelocytic leukemia cellsClinical relevanceHuman promyelocytic leukemia cellsProtein antigensIgMELISADogsLeukemia cellsAntibodiesBlot analysisAntigen
2000
Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens
Magnarelli L, Ijdo J, Padula S, Flavell R, Fikrig E. Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens. Journal Of Clinical Microbiology 2000, 38: 1735-1739. PMID: 10790090, PMCID: PMC86574, DOI: 10.1128/jcm.38.5.1735-1739.2000.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayHuman granulocytic ehrlichiosisRecombinant antigensClass-specific enzyme-linked immunosorbent assaysIgG antibodiesAntibody reactivityLyme borreliosisSerum samplesImmunoblotting of seraImmunoglobulin M antibodiesLow antibody titersB. burgdorferiEnzyme-linked immunosorbentWhole cell antigenAnalysis of serumWestern blot analysisOuter surface protein CErythema migransRheumatoid arthritisAntibody titersIgM antibodiesM antibodiesBorrelia burgdorferi sensu strictoOral infectionSurface protein C
1999
ANTIBODIES TO GRANULOCYTIC EHRLICHIAE IN WHITE-FOOTED AND COTTON MICE IN EASTERN UNITED STATES
Magnarelli L, Stafford K, IJdo J, Fikrig E, Oliver J, Hutcheson H, Boone J. ANTIBODIES TO GRANULOCYTIC EHRLICHIAE IN WHITE-FOOTED AND COTTON MICE IN EASTERN UNITED STATES. Journal Of Wildlife Diseases 1999, 35: 259-265. PMID: 10231752, DOI: 10.7589/0090-3558-35.2.259.Peer-Reviewed Original ResearchConceptsIndirect fluorescent antibodyNCH-1 strainHuman granulocytic ehrlichiosis (HGE) agentGranulocytic ehrlichiaeWestern blot analysisP. gossypinusSerologic assaysImmune responseWestern blot procedureEtiologic agentMouse serumSurveillance programSerum samplesMiceSerumAntibodiesEndemic sitesFluorescent antibodyBlot analysisWhite-footed miceIFA methodTitersEhrlichiaeP. leucopusAmelia Island
1998
Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays
Magnarelli L, IJdo J, Dumler J, Heimer R, Fikrig E. Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays. The Journal Of Infectious Diseases 1998, 178: 1835-1838. PMID: 9815246, DOI: 10.1086/314516.Peer-Reviewed Original ResearchConceptsNCH-1 strainIndirect IFAHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisWestern blot analysisInfected neutrophilsPromyelocytic leukemia cellsHuman promyelocytic leukemia cellsAntibody detectionGranulocytic ehrlichiosisEhrlichia equiDifferent strainsHuman isolatesLeukemia cellsSerumImmunodiagnostic assaysBlot analysisGranulocytic ehrlichiaeIFA analysisHuman serumIFAEhrlichiaeAssaysSeropositivityPatientsHuman Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut
Magnarelli L, Ijdo J, Anderson J, Padula S, Flavell R, Fikrig E. Human Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut. Journal Of Clinical Microbiology 1998, 36: 2823-2827. PMID: 9738027, PMCID: PMC105071, DOI: 10.1128/jcm.36.10.2823-2827.1998.Peer-Reviewed Original ResearchConceptsHuman monocytic ehrlichiosisWestern blot analysisTick-borne agentsProbable human casesTick-borne infectionsHuman granulocytic ehrlichiosis (HGE) agentBlot analysisSerologic testingMarked leukopeniaSeropositive specimensPositive seraHGE agentLyme borreliosisBabesia microtiHuman casesMonocytic ehrlichiosisEhrlichia equiBorrelia burgdorferiGranulocytic EhrlichiaEhrlichia chaffeensisFrequent detectionHuman exposurePatientsInfectionAntibodies
1997
Antibodies to Ehrlichia equi in dogs from the northeastern United States.
Magnarelli L, IJdo J, Anderson J, Madigan J, Dumler J, Fikrig E. Antibodies to Ehrlichia equi in dogs from the northeastern United States. Journal Of The American Veterinary Medical Association 1997, 211: 1134-7. PMID: 9364226, DOI: 10.2460/javma.1997.211.09.1134.Peer-Reviewed Original ResearchConceptsE equiIndirect fluorescent antibodyWestern blot analysisSerologic testing methodsFluorescent antibodyBlot analysisTick-infested areasSeropositive dogsIll dogsMarked leukopeniaNormal dogsSubclinical infectionSerologic analysisEtiologic agentLaboratory diagnosisEnd pointGranulocytic ehrlichiosisEhrlichia equiDogsAntibodiesHuman isolatesBorrelia burgdorferiDog serumMultiple pathogensEhrlichiosisSerologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays
Magnarelli LA, Flavell RA, Padula SJ, Anderson JF, Fikrig E. Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays. Journal Of Clinical Microbiology 1997, 35: 169-173. PMID: 8968901, PMCID: PMC229532, DOI: 10.1128/jcm.35.1.169-173.1997.Peer-Reviewed Original ResearchConceptsRecombinant antigensPolyvalent enzyme-linked immunosorbent assaysWhole-cell B. burgdorferiEnzyme-linked immunosorbent assayWestern blot analysisOuter surface protein ASensu strictoBlot analysisWhole cellsProtein AOSPFSerologic diagnosisBorrelia burgdorferi sensu strictoEquid seraBurgdorferi sensu strictoB. burgdorferiSurface protein AP41OspCWhole cell antigenEquine serumDog serumImmunosorbent assayOspBSerum samples
1995
The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease.
Jwang B, Dewing P, Fikrig E, Flavell RA. The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease. MSphere 1995, 2: 609-15. PMID: 8548542, PMCID: PMC170207, DOI: 10.1128/cdli.2.5.609-615.1995.Peer-Reviewed Original ResearchConceptsFlgE proteinFlgE geneEscherichia coli expression plasmidPrimary protein sequenceB. burgdorferi N40Hook geneWhole cell lysatesHook proteinLinear chromosomesPeriplasmic flagellaProtein sequencesFlgERecombinant proteinsB. burgdorferiBasal bodiesFlagellar filamentsWestern blot analysisBorrelia burgdorferiGenesExpression plasmidProteinBlot analysisNegative bacteriaMurine antiseraLyme disease