2001
Recombinant Protein-44-Based Class-Specific Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Human Granulocytic Ehrlichiosis
Magnarelli L, IJdo J, Wu C, Fikrig E. Recombinant Protein-44-Based Class-Specific Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Human Granulocytic Ehrlichiosis. European Journal Of Clinical Microbiology & Infectious Diseases 2001, 20: 482-485. PMID: 11561804, DOI: 10.1007/s100960100542.Peer-Reviewed Original ResearchConceptsPolyvalent enzyme-linked immunosorbent assaysClass-specific enzyme-linked immunosorbent assaysEnzyme-linked immunosorbent assayIndirect fluorescent-antibody analysisFluorescent antibody analysisImmunosorbent assaySpecificity testsIgG antibodiesHigh sensitivityHuman granulocytic ehrlichiosisHuman serumGranulocytic ehrlichiosisWhole cell antigenAntibody analysisImmunoglobulin M antibodiesOnset of illnessSerologic diagnosisSerum samplesHuman granulocytic ehrlichiosis (HGE) agentPatient 3False-positive resultsIgM antibodiesM antibodiesTotal immunoglobulinTotal antibody
1997
Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays
Magnarelli LA, Flavell RA, Padula SJ, Anderson JF, Fikrig E. Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays. Journal Of Clinical Microbiology 1997, 35: 169-173. PMID: 8968901, PMCID: PMC229532, DOI: 10.1128/jcm.35.1.169-173.1997.Peer-Reviewed Original ResearchConceptsRecombinant antigensPolyvalent enzyme-linked immunosorbent assaysWhole-cell B. burgdorferiEnzyme-linked immunosorbent assayWestern blot analysisOuter surface protein ASensu strictoBlot analysisWhole cellsProtein AOSPFSerologic diagnosisBorrelia burgdorferi sensu strictoEquid seraBurgdorferi sensu strictoB. burgdorferiSurface protein AP41OspCWhole cell antigenEquine serumDog serumImmunosorbent assayOspBSerum samples
1995
Evaluation of a lyme disease enzyme immunoassay using the 41-G fragment of flagellin
Cretella S, Gordon S, Flavell RA, Fikrig E. Evaluation of a lyme disease enzyme immunoassay using the 41-G fragment of flagellin. European Journal Of Clinical Microbiology & Infectious Diseases 1995, 14: 609-613. PMID: 7588849, DOI: 10.1007/bf01690736.Peer-Reviewed Original Research
1993
Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi
Fikrig E, Magnarelli LA, Chen M, Anderson JF, Flavell RA. Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi. Journal Of Clinical Microbiology 1993, 31: 2451-2455. PMID: 7691874, PMCID: PMC265777, DOI: 10.1128/jcm.31.9.2451-2455.1993.Peer-Reviewed Original Research
1992
Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and Band Flagellin
Fikrig E, Huguenel E, Berland R, Rahn D, Hardin J, Flavell R. Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and Band Flagellin. The Journal Of Infectious Diseases 1992, 165: 1127-1132. PMID: 1583333, DOI: 10.1093/infdis/165.6.1127.Peer-Reviewed Original ResearchConceptsLyme diseaseRecombinant outer surface proteinRecombinant outer surface protein AB. burgdorferi antigensSurface protein AOuter surface protein AEarly diseaseLate diseaseSerologic diagnosisDiagnostic testingOuter surface proteinsImmunogenic regionPatientsDiseaseBorrelia burgdorferiELISAImmunoblotAntibodiesSurface proteinsFlagellinProtein AAntigenDiagnosis