2007
A West Nile Virus Recombinant Protein Vaccine That Coactivates Innate and Adaptive Immunity
Huleatt J, Foellmer H, Hewitt D, Tang J, Desai P, Price A, Jacobs A, Takahashi V, Huang Y, Nakaar V, Alexopoulou L, Fikrig E, Powell T, McDonald W. A West Nile Virus Recombinant Protein Vaccine That Coactivates Innate and Adaptive Immunity. The Journal Of Infectious Diseases 2007, 195: 1607-1617. PMID: 17471430, DOI: 10.1086/517613.Peer-Reviewed Original ResearchConceptsImmune responseAntibody responseImmunoglobulin G antibody responseC3H/HeN miceWest Nile virus vaccineAdaptive immune signalsTLR5-deficient miceAntigen-specific responsesG antibody responseProtective immune responseAdaptive immune responsesToll-like receptorsWNV envelope proteinLethal WNV challengeRecombinant protein vaccineInterleukin-8 productionNeutralized viral infectivityEnzyme-linked immunosorbentEffective WNV vaccinesWNV vaccineHeN miceVirus challengeProtein vaccineVirus vaccineWNV challenge
2005
Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens
Magnarelli L, Fikrig E. Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens. Research In Veterinary Science 2005, 79: 99-103. PMID: 15924926, DOI: 10.1016/j.rvsc.2004.11.009.Peer-Reviewed Original Research
2004
Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease
Fikrig E, Coyle PK, Schutzer SE, Chen M, Deng Z, Flavell RA. Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease. Journal Of Clinical Microbiology 2004, 42: 1243-1246. PMID: 15004083, PMCID: PMC356844, DOI: 10.1128/jcm.42.3.1243-1246.2004.Peer-Reviewed Original ResearchDetection of Human Anti-Flavivirus Antibodies with a West Nile Virus Recombinant Antigen Microsphere Immunoassay
Wong SJ, Demarest VL, Boyle RH, Wang T, Ledizet M, Kar K, Kramer LD, Fikrig E, Koski RA. Detection of Human Anti-Flavivirus Antibodies with a West Nile Virus Recombinant Antigen Microsphere Immunoassay. Journal Of Clinical Microbiology 2004, 42: 65-72. PMID: 14715733, PMCID: PMC321652, DOI: 10.1128/jcm.42.1.65-72.2004.Peer-Reviewed Original ResearchConceptsCerebrospinal fluidWN virusRelated flavivirusesProtein antigensMicrosphere immunoassayAnti-flavivirus antibodiesAnti-human immunoglobulin antibodiesNew York patientsImmunoglobulin G antibodiesPublic health workersWN virus infectionEnzyme-linked immunosorbent assayEnzyme-linked immunosorbentE proteinSt. Louis encephalitisWest Nile virusViral encephalitisIgM antibodiesAnti-IgG antibodiesManagement of outbreaksG antibodiesVirus infectionHealth workersJapanese encephalitisImmunoglobulin antibodies
2002
Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis
Magnarelli LA, Lawrenz M, Norris SJ, Fikrig E. Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. Journal Of Medical Microbiology 2002, 51: 649-655. PMID: 12171295, DOI: 10.1099/0022-1317-51-8-649.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayIgG antibodiesErythema migransIgM antibodiesLyme borreliosisPhysician-diagnosed erythema migransOnset of illnessVlsE antigenB. burgdorferi infectionBorrelia burgdorferi antigensEnzyme-linked immunosorbentHuman granulocytic ehrlichiosisHuman serumOuter surface protein CRheumatoid arthritisBorrelia burgdorferi sensu strictoOral infectionBurgdorferi infectionFalse-positive reactionsBurgdorferi sensu strictoSera crossLouse-borneLaboratory diagnosisNormal serumGranulocytic ehrlichiosis
2001
Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Oliver JH, Fikrig E. Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis. American Journal Of Veterinary Research 2001, 62: 1365-9. PMID: 11560261, DOI: 10.2460/ajvr.2001.62.1365.Peer-Reviewed Original ResearchConceptsIgG antibodiesSerum samplesHGE agentGranulocytic ehrlichiosisClient-owned dogsDog serum samplesHuman granulocytic ehrlichiosis (HGE) agentEnzyme-linked immunosorbentPositive resultsWestern blot analysisTick-infested areasEhrlichia phagocytophila genogroupTotal immunoglobulinHorse serum samplesPromyelocytic leukemia cellsClinical relevanceHuman promyelocytic leukemia cellsProtein antigensIgMELISADogsLeukemia cellsAntibodiesBlot analysisAntigenOspA Immunization Decreases Transmission of Borrelia burgdorferi Spirochetes from Infected Peromyscus leucopus Mice to Larval Ixodes scapularis Ticks
Tsao J, Barbour A, Luke C, Fikrig E, Fish D. OspA Immunization Decreases Transmission of Borrelia burgdorferi Spirochetes from Infected Peromyscus leucopus Mice to Larval Ixodes scapularis Ticks. Vector-Borne And Zoonotic Diseases 2001, 1: 65-74. PMID: 12653137, DOI: 10.1089/153036601750137705.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, WildAntibodies, BacterialAntigens, SurfaceArachnid VectorsBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferiDisease ReservoirsDose-Response Relationship, ImmunologicEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, DirectHost-Parasite InteractionsIxodesLarvaLipoproteinsLyme DiseasePeromyscusConceptsControl miceUninfected larval ticksInfection prevalenceLarval Ixodes scapularis ticksB. burgdorferiNumber of spirochetesEnzyme-linked immunosorbentBorrelia burgdorferi spirochetesLevel of immunizationReservoir hostsConcentration of antibodyBorrelia burgdorferi transmissionIxodes scapularis ticksSecond vaccinationImmunized miceInfected miceLarval ticksPrior infectionVaccination programSubsequent riskDecrease transmissionOspA vaccinationDirect immunofluorescenceImmune responseXenodiagnostic ticks
2000
Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens
Magnarelli L, Ijdo J, Padula S, Flavell R, Fikrig E. Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens. Journal Of Clinical Microbiology 2000, 38: 1735-1739. PMID: 10790090, PMCID: PMC86574, DOI: 10.1128/jcm.38.5.1735-1739.2000.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayHuman granulocytic ehrlichiosisRecombinant antigensClass-specific enzyme-linked immunosorbent assaysIgG antibodiesAntibody reactivityLyme borreliosisSerum samplesImmunoblotting of seraImmunoglobulin M antibodiesLow antibody titersB. burgdorferiEnzyme-linked immunosorbentWhole cell antigenAnalysis of serumWestern blot analysisOuter surface protein CErythema migransRheumatoid arthritisAntibody titersIgM antibodiesM antibodiesBorrelia burgdorferi sensu strictoOral infectionSurface protein C
1999
Influence of Outer Surface Protein A Antibody onBorrelia burgdorferi within Feeding Ticks
de Silva A, Zeidner N, Zhang Y, Dolan M, Piesman J, Fikrig E. Influence of Outer Surface Protein A Antibody onBorrelia burgdorferi within Feeding Ticks. Infection And Immunity 1999, 67: 30-35. PMID: 9864192, PMCID: PMC96273, DOI: 10.1128/iai.67.1.30-35.1999.Peer-Reviewed Original ResearchConceptsAntibody levelsSalivary glandsAntibody titersOspA antibodiesBorrelia burgdorferiLow antibody levelsNumber of spirochetesDensity of spirochetesEnzyme-linked immunosorbentFeeding ticksSurface protein AAbility of spirochetesOuter surface protein AProtective epitopesCompetitive enzyme-linked immunosorbentOuter surface proteinsSpirochetal agentLyme diseaseAntibodiesB. burgdorferiTitersIxodes ticksGlandBurgdorferiSpirochetes
1997
Borrelia burgdorferi enzyme-linked immunosorbent assay for discrimination of OspA vaccination from spirochete infection
Zhang YQ, Mathiesen D, Kolbert CP, Anderson J, Schoen RT, Fikrig E, Persing DH. Borrelia burgdorferi enzyme-linked immunosorbent assay for discrimination of OspA vaccination from spirochete infection. Journal Of Clinical Microbiology 1997, 35: 233-238. PMID: 8968914, PMCID: PMC229545, DOI: 10.1128/jcm.35.1.233-238.1997.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayVaccine failureFalse positivityAntigen preparationsExperimental animalsNatural infectionLyme disease vaccineB. burgdorferiExperimental animal modelsNegative enzyme-linked immunosorbent assayEnzyme-linked immunosorbentWhole-cell sonicateSurface protein AVaccinated subjectsLyme disease casesOuter surface protein AVaccinated personsFalse-positive resultsVaccine trialsOspA vaccinationSpirochete infectionDisease vaccineAnimal modelsHuman Lyme disease casesNegative isolates
1995
REACTIVITY OF WHITE-FOOTED MOUSE AND RACCOON SERA TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI
Magnarelli L, Anderson J, Flavell R, Fikrig E. REACTIVITY OF WHITE-FOOTED MOUSE AND RACCOON SERA TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI. Journal Of Wildlife Diseases 1995, 31: 339-344. PMID: 8592354, DOI: 10.7589/0090-3558-31.3.339.Peer-Reviewed Original ResearchConceptsWhole cell antigenCell antigensRecombinant antigensSpecific recombinant antigensGeometric mean antibody titersMean antibody titersPresence of antibodiesB. burgdorferi infectionEnzyme-linked immunosorbentWhite-footed miceAntibody titersBorrelia burgdorferi sensu strictoBurgdorferi infectionBurgdorferi sensu stricto
1993
Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi
Fikrig E, Magnarelli LA, Chen M, Anderson JF, Flavell RA. Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi. Journal Of Clinical Microbiology 1993, 31: 2451-2455. PMID: 7691874, PMCID: PMC265777, DOI: 10.1128/jcm.31.9.2451-2455.1993.Peer-Reviewed Original Research