2021
Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Li ML, Luo J, Ellis MW, Riaz M, Ajaj Y, Qyang Y. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells. Methods In Molecular Biology 2021, 2375: 21-34. PMID: 34591296, DOI: 10.1007/978-1-0716-1708-3_3.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsVascular smooth muscle cellsPluripotent stem cellsLateral plate mesodermEarly embryonic developmentStem cellsSmooth muscle cellsHuman pluripotent stem cellsInduced pluripotent stem cellsExtracellular matrix proteinsMuscle cellsMesoderm lineagePlate mesodermEmbryonic developmentVascular cell sourceEmbryoid bodiesEB formationMatrix proteinsCellular interactionsDisease modelingPhysiological characteristicsVascular tissueTissue-engineered vascular graftsCell-based therapiesCell replacement
2020
Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering
Luo J, Lin Y, Shi X, Li G, Kural MH, Anderson CW, Ellis MW, Riaz M, Tellides G, Niklason LE, Qyang Y. Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering. Acta Biomaterialia 2020, 119: 155-168. PMID: 33130306, PMCID: PMC8168373, DOI: 10.1016/j.actbio.2020.10.042.Peer-Reviewed Original ResearchConceptsVascular tissue engineeringTissue-engineered vascular graftsTissue engineeringComparable mechanical strengthVascular smooth muscle cellsMechanical strengthSmooth muscle cellsPolyglycolic acid scaffoldsTechnology one stepBiodegradable polyglycolic acid (PGA) scaffoldsXenogeneic-free conditionsAnimal-derived reagentsMuscle cellsVSMC differentiationImmunodeficient mouse modelEngineeringVascular graftsOne-stepStem cellsPluripotent stem cellsMouse modelCollagen depositionComparable capacityBlood vesselsAcid scaffoldsTissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs
Luo J, Qin L, Zhao L, Gui L, Ellis MW, Huang Y, Kural MH, Clark JA, Ono S, Wang J, Yuan Y, Zhang SM, Cong X, Li G, Riaz M, Lopez C, Hotta A, Campbell S, Tellides G, Dardik A, Niklason LE, Qyang Y. Tissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs. Cell Stem Cell 2020, 26: 251-261.e8. PMID: 31956039, PMCID: PMC7021512, DOI: 10.1016/j.stem.2019.12.012.Peer-Reviewed Original Research