2007
Induction of Vascular Permeability: βPIX and GIT1 Scaffold the Activation of Extracellular Signal-regulated Kinase by PAK
Stockton R, Reutershan J, Scott D, Sanders J, Ley K, Schwartz MA. Induction of Vascular Permeability: βPIX and GIT1 Scaffold the Activation of Extracellular Signal-regulated Kinase by PAK. Molecular Biology Of The Cell 2007, 18: 2346-2355. PMID: 17429073, PMCID: PMC1877103, DOI: 10.1091/mbc.e06-07-0584.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCapillary PermeabilityCattleCell Cycle ProteinsCells, CulturedEndothelial CellsEnzyme ActivationExtracellular Signal-Regulated MAP KinasesGuanine Nucleotide Exchange FactorsHumansInflammationLipopolysaccharidesLungMiceP21-Activated KinasesPeptidesProtein Serine-Threonine KinasesRho Guanine Nucleotide Exchange FactorsConceptsP21-activated kinaseMitogen-activated protein kinase kinaseEndothelial cell-cell junctionsExtracellular signal-regulated kinaseCell-cell junctionsProtein kinase kinaseMyosin light chain phosphorylationLight chain phosphorylationSignal-regulated kinaseCell-permeant peptideActivation of ERKKinase kinaseExtracellular signalsPAK functionChain phosphorylationCritical regulatorKinaseCell contractilityCell typesCultured endothelial cellsPhosphorylationMouse lung injury modelMyosin phosphorylationEndothelial cellsGIT1
2006
High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry
Lechene C, Hillion F, McMahon G, Benson D, Kleinfeld A, Kampf J, Distel D, Luyten Y, Bonventre J, Hentschel D, Park K, Ito S, Schwartz M, Benichou G, Slodzian G. High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry. BMC Biology 2006, 5: 20. PMID: 17010211, PMCID: PMC1781526, DOI: 10.1186/jbiol42.Peer-Reviewed Original ResearchConceptsMulti-isotope imaging mass spectrometryMammalian cultured cellsFatty acid transportAdipocyte lipid dropletsHigh-resolution quantitative imagingSubcellular compartmentsNitrogen fixationMass spectrometryImaging mass spectrometryLevel of enrichmentBacterial cellsProtein renewalCultured endothelial cellsCultured cellsLipid dropletsKidney cellsStable isotopesUridine incorporationEndothelial cellsCellsQuantitative image analysis softwareStable isotope mass spectrometryIsotopic labelsIsotope ratiosRNA