2013
Development of an assay to measure mutagenic non-homologous end-joining repair activity in mammalian cells
Bindra RS, Goglia AG, Jasin M, Powell SN. Development of an assay to measure mutagenic non-homologous end-joining repair activity in mammalian cells. Nucleic Acids Research 2013, 41: e115-e115. PMID: 23585275, PMCID: PMC3675474, DOI: 10.1093/nar/gkt255.Peer-Reviewed Original ResearchConceptsMammalian cellsHomologous recombinationDouble-strand break repair pathwayNon-homologous end-joining repairSite-specific DSBsEnd-joining activityBreak repair pathwayEnd-joining repairPrevention of tumorigenesisSerum-deprived cellsGenomic integrityIntrachromosomal locusI-SceIHR repairProtein stabilityCanonical NHEJRepair pathwaysRepair assaysNHEJ repairMutagenic NHEJCellular localizationDSB inductionLiving cellsNHEJRepair activity
2010
Inhibition of poly(ADP-ribose) polymerase down-regulates BRCA1 and RAD51 in a pathway mediated by E2F4 and p130
Hegan DC, Lu Y, Stachelek GC, Crosby ME, Bindra RS, Glazer PM. Inhibition of poly(ADP-ribose) polymerase down-regulates BRCA1 and RAD51 in a pathway mediated by E2F4 and p130. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 2201-2206. PMID: 20133863, PMCID: PMC2836641, DOI: 10.1073/pnas.0904783107.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorColonic NeoplasmsCrk-Associated Substrate ProteinDNA RepairDown-RegulationE2F4 Transcription FactorEnzyme InhibitorsGenes, BRCA1HumansPhenanthrenesPoly (ADP-Ribose) Polymerase-1Poly(ADP-ribose) Polymerase InhibitorsPoly(ADP-ribose) PolymerasesPromoter Regions, GeneticRad51 RecombinaseRadiation-Sensitizing AgentsRNA, Small InterferingConceptsHomology-dependent repairBase excision repair factorsExcision repair factorsPARP inhibitionRole of PARPPARP inhibitorsRepair factorsExpression of BRCA1DNA repairDNA breaksHypoxic cancer cellsRAD51SiRNA knockdownDNA damagePARP-1P130 expressionCancer therapyP130Cancer cellsPARPRad51 promoterHPV E7BRCA1E7 expressionSiRNAs