2021
Norovirus evolution in immunodeficient mice reveals potentiated pathogenicity via a single nucleotide change in the viral capsid
Walker FC, Hassan E, Peterson ST, Rodgers R, Schriefer LA, Thompson CE, Li Y, Kalugotla G, Blum-Johnston C, Lawrence D, McCune BT, Graziano VR, Lushniak L, Lee S, Roth AN, Karst SM, Nice TJ, Miner JJ, Wilen CB, Baldridge MT. Norovirus evolution in immunodeficient mice reveals potentiated pathogenicity via a single nucleotide change in the viral capsid. PLOS Pathogens 2021, 17: e1009402. PMID: 33705489, PMCID: PMC7987144, DOI: 10.1371/journal.ppat.1009402.Peer-Reviewed Original ResearchConceptsNucleotide changesSingle nucleotide changeViral capsidAmino acid changesEvolutionary potentialIFN-competent hostsIntestinal myeloid cellsSelective pressureSingle nucleotideKey controllerNorovirus evolutionAcid changesLethal pathogenVirus growthEnhanced virulenceMice revealsIFN responseHigh replicationEnhanced recruitmentMyeloid cellsExtraintestinal disseminationIntestinal replicationReplicationPathogenicityCapsid
2020
Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity
Brestoff JR, Wilen CB, Moley JR, Li Y, Zou W, Malvin NP, Rowen MN, Saunders BT, Ma H, Mack MR, Hykes BL, Balce DR, Orvedahl A, Williams JW, Rohatgi N, Wang X, McAllaster MR, Handley SA, Kim BS, Doench JG, Zinselmeyer BH, Diamond MS, Virgin HW, Gelman AE, Teitelbaum SL. Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity. Cell Metabolism 2020, 33: 270-282.e8. PMID: 33278339, PMCID: PMC7858234, DOI: 10.1016/j.cmet.2020.11.008.Peer-Reviewed Original ResearchConceptsIntercellular mitochondria transferMitochondria transferMitochondria uptakeMetabolic homeostasisGenome-wide CRISPRWhite adipose tissue homeostasisWAT macrophagesDistinct macrophage subpopulationsKnockout screensTissue homeostasisHeparan sulfateAdipose tissue homeostasisWhite adipose tissueGenes EXT1HomeostasisImmunometabolic crosstalkMitochondriaAdipocytesMyeloid cellsMacrophage subpopulationsCellsVivoRecent studiesMacrophagesCRISPRSelect autophagy genes maintain quiescence of tissue-resident macrophages and increase susceptibility to Listeria monocytogenes
Wang YT, Zaitsev K, Lu Q, Li S, Schaiff WT, Kim KW, Droit L, Wilen CB, Desai C, Balce DR, Orchard RC, Orvedahl A, Park S, Kreamalmeyer D, Handley SA, Pfeifer JD, Baldridge MT, Artyomov MN, Stallings CL, Virgin HW. Select autophagy genes maintain quiescence of tissue-resident macrophages and increase susceptibility to Listeria monocytogenes. Nature Microbiology 2020, 5: 272-281. PMID: 31959973, PMCID: PMC7147835, DOI: 10.1038/s41564-019-0633-0.Peer-Reviewed Original ResearchConceptsTissue-resident macrophagesAutophagy genesDegradative autophagyBeclin-1Maintenance of proteinMyeloid cells resultsAutophagy protein Beclin 1Protein Beclin 1Organelle integrityCellular processesMyeloid cellsBacterial microbiotaCytoplasmic contentsLysosomal digestionGenesCommensal microorganismsCells resultsAutophagyFIP200Homeostatic functionsListeria monocytogenes infectionAdaptive immune responsesKey functionsMice displayMacrophage response