2024
Performance of two modified two-tier algorithms for the serologic diagnosis of Lyme disease
Landry M, Hassan S, Rottmann B, Pesak S, Ordazzo M, Skrzyniarz M, Deponte S, Peaper D. Performance of two modified two-tier algorithms for the serologic diagnosis of Lyme disease. Journal Of Clinical Microbiology 2024, 62: e00139-24. PMID: 38597655, PMCID: PMC11077974, DOI: 10.1128/jcm.00139-24.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAntibodies, BacterialHumansImmunoblottingImmunoglobulin GImmunoglobulin MLuminescent MeasurementsLyme DiseaseSensitivity and SpecificitySerologic TestsConceptsPositive percent agreementSerologic diagnosis of Lyme diseaseDiagnosis of Lyme diseaseEnzyme-linked immunoassayBorrelia speciesTwo-tier algorithmLyme diseaseTwo-tier testImmunoblottingLaboratory diagnosis of Lyme diseaseLIAISON chemiluminescent immunoassaysChemiluminescence immunoassayDetection of IgMScreening testConsensus reference standardSerological diagnosisLaboratory diagnosisDiaSorinIgM resultsIgM detection
2020
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
Vogels CBF, Brito AF, Wyllie AL, Fauver JR, Ott IM, Kalinich CC, Petrone ME, Casanovas-Massana A, Catherine Muenker M, Moore AJ, Klein J, Lu P, Lu-Culligan A, Jiang X, Kim DJ, Kudo E, Mao T, Moriyama M, Oh JE, Park A, Silva J, Song E, Takahashi T, Taura M, Tokuyama M, Venkataraman A, Weizman OE, Wong P, Yang Y, Cheemarla NR, White EB, Lapidus S, Earnest R, Geng B, Vijayakumar P, Odio C, Fournier J, Bermejo S, Farhadian S, Dela Cruz CS, Iwasaki A, Ko AI, Landry ML, Foxman EF, Grubaugh ND. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets. Nature Microbiology 2020, 5: 1299-1305. PMID: 32651556, PMCID: PMC9241364, DOI: 10.1038/s41564-020-0761-6.Peer-Reviewed Original ResearchConceptsSARS-CoV-2SARS-CoV-2 RTSevere acute respiratory syndrome coronavirusAcute respiratory syndrome coronavirusViral RNA copiesPublic health laboratoriesPublic health interventionsReverse transcription-PCR assaySARS-CoV-2 diagnostic testingDiagnostic assaysTranscription-PCR assaySARS-CoV-2 evolutionQuantitative reverse transcription-PCR assaysRapid diagnostic assaysHealth laboratoriesHealth interventionsDiagnostic testingRNA copiesPrimer-probe setsAssaysLow sensitivityCritical needAnalytical sensitivityHigh Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing
Landry ML, Topal JE, Estis J, Katzenbach P, Nolan N, Sandlund J. High Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing. Journal Of Clinical Microbiology 2020, 58: 10.1128/jcm.01629-19. PMID: 31776192, PMCID: PMC6989068, DOI: 10.1128/jcm.01629-19.Peer-Reviewed Original ResearchConceptsToxin enzyme immunoassayToxin A/BCell cytotoxicity neutralization assayEnzyme immunoassayStool samplesDifficile toxin assaysProspective clinical studyCytotoxicity neutralization assayCare algorithmChart reviewClinical studiesToxin ACytotoxin testingNeutralization assaysToxin assaysNegative agreementDiscordant samplesLaboratory algorithmTesting algorithmHigh agreementInfection diagnosticsGlutamate dehydrogenasePositive agreementImmunoassayAssays
2016
Laboratory Diagnosis of Zika Virus Infection
Landry ML, St George K. Laboratory Diagnosis of Zika Virus Infection. Archives Of Pathology & Laboratory Medicine 2016, 141: 60-67. PMID: 27763787, DOI: 10.5858/arpa.2016-0406-sa.Peer-Reviewed Original ResearchConceptsNucleic acid amplification testsZika virus infectionZika infectionVirus infectionAmplification testsLaboratory diagnosisNucleic acid amplification testingCommercial nucleic acid amplification testsAccurate antibody testZika virus testingPublic health recommendationsEmergency use authorizationAcute infectionAmplification testingSerology resultsPast infectionFlavivirus antigensHealth recommendationsVirus testingCurrent recommendationsAntibody testApplicable diagnostic toolsVirus epitopesAccurate diagnosisInfectionImmunoglobulin M for Acute Infection: True or False?
Landry ML. Immunoglobulin M for Acute Infection: True or False? MSphere 2016, 23: 540-545. PMID: 27193039, PMCID: PMC4933779, DOI: 10.1128/cvi.00211-16.Peer-Reviewed Original ResearchMeSH KeywordsCommunicable DiseasesFalse Positive ReactionsHumansImmunoglobulin MSensitivity and SpecificitySerologic TestsConceptsClear clinical utilityFalse-positive IgMImmunoglobulin M testAcute infectionInappropriate therapyFalse-positive resultsDiagnostic workupEpidemiologic findingsIgM detectionInfection controlClinical utilityImmunoglobulin MSerial samplesAccurate diagnosisPremature closurePublic healthTherapyNumerous reportsDiagnosisPatientsPrognosisIgMWorkupInfectionClinicians
2014
A Highly Sensitive Europium Nanoparticle-Based Immunoassay for Detection of Influenza A/B Virus Antigen in Clinical Specimens
Zhang P, Vemula SV, Zhao J, Du B, Mohan H, Liu J, Mubarak H, Landry ML, Hewlett I. A Highly Sensitive Europium Nanoparticle-Based Immunoassay for Detection of Influenza A/B Virus Antigen in Clinical Specimens. Journal Of Clinical Microbiology 2014, 52: 4385-4387. PMID: 25297327, PMCID: PMC4313323, DOI: 10.1128/jcm.02635-14.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, ViralDiagnostic Tests, RoutineEuropiumHumansImmunoassayInfluenza A virusInfluenza B virusInfluenza, HumanNanoparticlesSensitivity and SpecificityRapid Diagnosis of Influenza State of the Art
Peaper DR, Landry ML. Rapid Diagnosis of Influenza State of the Art. Clinics In Laboratory Medicine 2014, 34: 365-385. PMID: 24856533, PMCID: PMC7172071, DOI: 10.1016/j.cll.2014.02.009.Peer-Reviewed Original ResearchComparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients
Landry ML, Ferguson D. Comparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients. Journal Of Clinical Microbiology 2014, 52: 3057-3059. PMID: 24850350, PMCID: PMC4136128, DOI: 10.1128/jcm.00738-14.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overDiagnostic Tests, RoutineFluorescent Antibody TechniqueHospitalsHumansInfluenza, HumanMiddle AgedNasopharynxPolymerase Chain ReactionSensitivity and Specificity
2013
Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease
Landry ML, Ferguson D, Topal J. Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease. Journal Of Clinical Microbiology 2013, 52: 275-280. PMID: 24226924, PMCID: PMC3911468, DOI: 10.1128/jcm.02545-13.Peer-Reviewed Original ResearchConceptsAntigen-negative samplesGDH antigenToxigenic C. difficileC. difficileGDH-positive samplesClostridium difficile infectionGlutamate dehydrogenase antigenToxin B geneSemisolid stoolsC. difficile toxin B geneDifficile testingChart reviewNosocomial transmissionDifficile infectionDifficile diseaseReal-time PCRClinical consequencesMerit further studyRapid molecular diagnosisDifficile bacteriaAntigenPatientsDehydrogenase antigenCost avoidanceCytotoxicity assaysComparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory-Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens
Gitman MR, Ferguson D, Landry ML. Comparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory-Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens. Journal Of Clinical Microbiology 2013, 51: 3765-3769. PMID: 24006008, PMCID: PMC3889765, DOI: 10.1128/jcm.01413-13.Peer-Reviewed Original ResearchAdolescentAdultAgedAged, 80 and overChildChild, PreschoolClinical Laboratory TechniquesCosts and Cost AnalysisFemaleFluorescent Antibody TechniqueHerpes SimplexHumansInfantInfant, NewbornMaleMiddle AgedPolymerase Chain ReactionSensitivity and SpecificitySimplexvirusTime FactorsVirus CultivationYoung Adult
2011
Diagnostic tests for influenza infection
Landry ML. Diagnostic tests for influenza infection. Current Opinion In Pediatrics 2011, 23: 91-97. PMID: 21150446, DOI: 10.1097/mop.0b013e328341ebd9.Peer-Reviewed Original ResearchConceptsMolecular testingMultiplex molecular testRapid antigen testInfluenza infectionViral loadAntigen testPatient groupSeasonal influenzaImmunofluorescence testDiagnostic test performanceAntigenic changesNext pandemicRapid cultureDiagnostic testsMolecular testsMarked variabilityNegative resultsSame testDiagnostic methods
2009
Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus
Landry ML, Ferguson D. Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus. Journal Of Clinical Microbiology 2009, 48: 957-959. PMID: 20042632, PMCID: PMC2832425, DOI: 10.1128/jcm.01678-09.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsChildChild, PreschoolClinical Laboratory TechniquesFluorescent Antibody Technique, DirectHumansInfantInfant, NewbornInfluenza A Virus, H1N1 SubtypeInfluenza, HumanNasopharynxReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityVirologyYoung AdultConceptsNovel swine-origin influenza virusesSwine-origin influenza virusImmunofluorescence antibody testingReal-time reverse transcriptase PCRCycle threshold valuesPCR-positive resultsPediatric patientsCDC assayAntibody testingNasopharyngeal swabsReverse transcriptase-PCRSwine influenzaInfluenza virusTranscriptase-PCRPositive samplesPatientsPositive resultsInfluenzaSwabsCytospinsComparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection
Kvach EJ, Ferguson D, Riska PF, Landry ML. Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection. Journal Of Clinical Microbiology 2009, 48: 109-114. PMID: 19864479, PMCID: PMC2812270, DOI: 10.1128/jcm.01630-09.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, BacterialBacterial ProteinsBacterial ToxinsBacteriological TechniquesClinical Laboratory TechniquesClostridioides difficileEnterocolitis, PseudomembranousEnterotoxinsEnzyme-Linked Immunosorbent AssayGlutamate DehydrogenaseHumansNeutralization TestsPolymerase Chain ReactionReagent Kits, DiagnosticSensitivity and Specificity
2008
Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients
Landry ML, Cohen S, Ferguson D. Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients. Journal Of Clinical Virology 2008, 43: 148-151. PMID: 18639488, PMCID: PMC7173065, DOI: 10.1016/j.jcv.2008.06.006.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAntibodies, MonoclonalChildChild, PreschoolFluorescent Antibody Technique, DirectHospitalizationHumansImmunoassayInfantInfluenza A virusInfluenza B virusInfluenza, HumanMiddle AgedNasopharynxReagent Kits, DiagnosticReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityTaq PolymeraseTime FactorsVirus CultivationYoung AdultConceptsHospitalized patientsTaqMan RT-PCRRT-PCRInfluenza ARapid flu testsDiagnosis of influenzaInfluenza-positive samplesDetection of influenzaReal-time RT-PCRInfluenza BInfluenza diagnosisPatient managementReal-time PCRMore accurate testsPatientsBinaxTrue positive samplesRapid testClinical samplesPositive samplesFurther studiesHospitalAccurate testInfluenzaDiagnosis
2005
Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens
Landry ML, Garner R, Ferguson D. Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens. Journal Of Clinical Microbiology 2005, 43: 3136-3139. PMID: 16000425, PMCID: PMC1169110, DOI: 10.1128/jcm.43.7.3136-3139.2005.Peer-Reviewed Original ResearchConceptsNucleic acid sequence-based amplificationTime Nucleic Acid Sequence-Based AmplificationEV-positive samplesReal-time nucleic acid sequence-based amplificationDetection of enterovirusesEnterovirus RNAStool samplesCerebrospinal fluidNASBA assayClinical specimensEnterovirusesMolecular beacon technologyAssays
2004
Automated detection of immunofluorescently labeled cytomegalovirus‐infected cells in isolated peripheral blood leukocytes using decision tree analysis
Ladanyi A, Sher AC, Herlitz A, Bergsrud DE, Kraeft S, Kepros J, McDaid G, Ferguson D, Landry ML, Chen LB. Automated detection of immunofluorescently labeled cytomegalovirus‐infected cells in isolated peripheral blood leukocytes using decision tree analysis. Cytometry Part A 2004, 58A: 147-156. PMID: 15057968, DOI: 10.1002/cyto.a.20016.Peer-Reviewed Original ResearchConceptsPeripheral blood leukocytesBlood leukocytesPatient samplesDecision tree analysisImage cytometryCytomegalovirus-infected cellsCMV infectionCytomegalovirus infectionAntiviral therapyViral antigensCellular featuresCytospin preparationsLeukocytesInfectionCytometryManual microscopic analysisImage cytometerRare cellsCellsSimilar resultsPatientsCMVTherapyAntigenDiagnosis
2003
Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens
Landry ML, Garner R, Ferguson D. Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens. Journal Of Clinical Microbiology 2003, 41: 5006-5010. PMID: 14605131, PMCID: PMC262477, DOI: 10.1128/jcm.41.11.5006-5010.2003.Peer-Reviewed Original ResearchConceptsNucleic acid sequence-based amplificationNucliSens Basic KitRT-PCREnterovirus RNABasic KitEnterovirus-positive samplesReverse transcription-PCR assayTranscription-PCR assayNasopharyngeal samplesRT-PCR kitVirus isolationClinical specimensTranscription-PCRPositive samplesComparable sensitivityKitMolecular methodsEnteroviruses
2001
Evaluation of Biosite Triage Clostridium difficile Panel for Rapid Detection of Clostridium difficile in Stool Samples
Landry M, Topal J, Ferguson D, Giudetti D, Tang Y. Evaluation of Biosite Triage Clostridium difficile Panel for Rapid Detection of Clostridium difficile in Stool Samples. Journal Of Clinical Microbiology 2001, 39: 1855-1858. PMID: 11326003, PMCID: PMC88038, DOI: 10.1128/jcm.39.5.1855-1858.2001.Peer-Reviewed Original ResearchConceptsNegative predictive valuePositive predictive valueCommon antigenStool samplesPredictive valueC. difficile toxin AHigh negative predictive valueAntigen-positive patientsDifficile-negative patientsAntigen positive resultDifficile toxin ADifficile carriersContact precautionsAntibiotic treatmentToxin ACytotoxin titersTriage PanelUnnecessary useClostridium difficilePatients
2000
A Multisite Trial Comparing Two Cytomegalovirus (CMV) pp65 Antigenemia Test Kits, Biotest CMV Brite and Bartels/Argene CMV Antigenemia
St. George K, Boyd M, Lipson S, Ferguson D, Cartmell G, Falk L, Rinaldo C, Landry M. A Multisite Trial Comparing Two Cytomegalovirus (CMV) pp65 Antigenemia Test Kits, Biotest CMV Brite and Bartels/Argene CMV Antigenemia. Journal Of Clinical Microbiology 2000, 38: 1430-1433. PMID: 10747120, PMCID: PMC86458, DOI: 10.1128/jcm.38.4.1430-1433.2000.Peer-Reviewed Original ResearchConceptsPp65 antigenemiaTransplant recipientsHuman immunodeficiency virus-positive patientsImmunodeficiency virus-positive patientsBone marrow transplant recipientsCMV pp65 antigenemiaOrgan transplant recipientsVirus-positive patientsMarrow transplant recipientsPositive cell countsPeripheral blood leukocytesNegative predictive valueCMV antigenemiaBlood leukocytesAntigenemiaBlood specimensPredictive valueCell countMultisite trialCytomegalovirusStatistical differenceTotalTest kitRecipientsKit