2021
H3.1K27me1 maintains transcriptional silencing and genome stability by preventing GCN5-mediated histone acetylation
Dong J, LeBlanc C, Poulet A, Mermaz B, Villarino G, Webb KM, Joly V, Mendez J, Voigt P, Jacob Y. H3.1K27me1 maintains transcriptional silencing and genome stability by preventing GCN5-mediated histone acetylation. The Plant Cell 2021, 33: 961-979. PMID: 33793815, PMCID: PMC8226292, DOI: 10.1093/plcell/koaa027.Peer-Reviewed Original ResearchConceptsGenome stabilityGenomic instabilityHistone acetylationSAGA-like complexesMultiple lysine residuesArabidopsis GCN5ARABIDOPSIS TRITHORAXArabidopsis thalianaTranscriptional silencingHeterochromatin defectsDouble mutantDNA replicationEpigenetic mechanismsGCN5Molecular roleEssential functionsDiverse rolesMolecular mechanismsLysine residuesProtein 5AcetylationMutantsPlantsADA2bATXR6
2017
Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress
LeBlanc C, Zhang F, Mendez J, Lozano Y, Chatpar K, Irish V, Jacob Y. Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress. The Plant Journal 2017, 93: 377-386. PMID: 29161464, DOI: 10.1111/tpj.13782.Peer-Reviewed Original ResearchConceptsCRISPR/Green fluorescent protein (GFP) reporter geneCRISPR/Cas9 systemFluorescent protein reporter geneCRISPR/Cas9Off-target mutationsArabidopsis plantsEukaryotic genomesDifferent organismsSomatic tissuesCitrus plantsCas9 systemDNA breaksReporter geneTarget mutagenesisTargeted mutationsMutation rateMutagenesisImportance of temperatureArabidopsisHeat stressPlantsMutationsQuantitative assayEukaryotes