2019
In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens
Chow RD, Wang G, Ye L, Codina A, Kim HR, Shen L, Dong MB, Errami Y, Chen S. In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens. Nature Methods 2019, 16: 405-408. PMID: 30962622, PMCID: PMC6592845, DOI: 10.1038/s41592-019-0371-5.Peer-Reviewed Original Research
2015
Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA. Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis. Cell 2015, 160: 1246-1260. PMID: 25748654, PMCID: PMC4380877, DOI: 10.1016/j.cell.2015.02.038.Peer-Reviewed Original ResearchConceptsSingle guide RNAsGenome-wide CRISPR screenGenome-scale libraryCRISPR/Cas9-mediated lossLate-stage primary tumorsEffects of mutationsGenetic screenTumor growthCRISPR screensFunction screenMouse cancer cell linesCancer evolutionGene phenotypeDiverse phenotypesFunction mutationsCancer cell linesGenesCell linesPrimary screenCell poolSpecific lossPrimary tumor growthSmall poolMutationsPhenotype