2017
Colony Lysate Arrays for Proteomic Profiling of Drug-Tolerant Persisters of Cancer Cell
Kume K, Nishizuka SS. Colony Lysate Arrays for Proteomic Profiling of Drug-Tolerant Persisters of Cancer Cell. Analytical Chemistry 2017, 89: 8626-8631. PMID: 28753272, DOI: 10.1021/acs.analchem.7b01215.Peer-Reviewed Original ResearchConceptsDrug-tolerant persistersCancer cellsTranscription factorsProteomic profilingProteomic profilesLevels of proteinIndividual coloniesProtein array systemNumber of assaysSingle cellsProtein levelsCritical mechanismFunctional informationAlternative therapeutic targetsProteinTherapeutic targetFunctional heterogeneityCellsInitiation of relapseProfilingPersistersAgarose gelOct4ASTAT3Colonies
2015
Systematic Protein Level Regulation via Degradation Machinery Induced by Genotoxic Drugs
Kume K, Ishida K, Ikeda M, Takemoto K, Shimura T, Young L, Nishizuka SS. Systematic Protein Level Regulation via Degradation Machinery Induced by Genotoxic Drugs. Journal Of Proteome Research 2015, 15: 205-215. PMID: 26625007, DOI: 10.1021/acs.jproteome.5b00759.Peer-Reviewed Original ResearchConceptsDegradation machineryGenotoxic drugsProtein dynamicsProtein-level regulationProtein degradation systemProtein levelsDistinct proteinsApoptosis-related proteinsDegradation systemProtein arraysProteinLevel regulationProteasome inhibitorsMachineryComprehensive insightRegulationMG132Similar dynamicsPairsInhibitorsDynamicsDifferent concentrationsResponseSimilarityLevels
2014
A Compensatory Role of NF-κB to p53 in Response to 5-FU–Based Chemotherapy for Gastric Cancer Cell Lines
Endo F, Nishizuka SS, Kume K, Ishida K, Katagiri H, Ishida K, Sato K, Iwaya T, Koeda K, Wakabayashi G. A Compensatory Role of NF-κB to p53 in Response to 5-FU–Based Chemotherapy for Gastric Cancer Cell Lines. PLOS ONE 2014, 9: e90155. PMID: 24587255, PMCID: PMC3937424, DOI: 10.1371/journal.pone.0090155.Peer-Reviewed Original ResearchMeSH KeywordsAntimetabolites, AntineoplasticCell Line, TumorCodonDrug Resistance, NeoplasmFluorouracilGene Expression ProfilingGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansNF-kappa BProtein BindingProtein TransportStomach NeoplasmsTranscription Factor RelATumor Suppressor Protein p53ConceptsGastric cancer cell linesCancer cell linesNF-κBAdjuvant chemotherapyPrediction markersCell linesNF-κB-dependent mannerGastric cancer patientsKnockdown of RelANF-κB bindingArg homozygosityCurative resectionRelapse rateCancer patientsGastric cancerPrediction biomarkersChemotherapyP65 subunitTP53 knockdownChemotherapeutic efficacyProtein levelsCompensatory roleP53Target protein levelsTreatment