1979
Binding of mammalian ribosomes to ms2 phage rna reveals an overlapping gene encoding a lysis function
Atkins J, Steitz J, Anderson C, Model P. Binding of mammalian ribosomes to ms2 phage rna reveals an overlapping gene encoding a lysis function. Cell 1979, 18: 247-256. PMID: 498271, DOI: 10.1016/0092-8674(79)90044-8.Peer-Reviewed Original ResearchConceptsMammalian ribosomesLysis functionE. coli cell-free systemAmino acid polypeptideCoat protein geneAmino acid sequence analysisPartial amino acid sequence analysisMS2 phage RNAAcid sequence analysisCell-free systemLysis genesNonsense mutantsSynthetase geneUAA codonProtein geneAcid polypeptideSite mutantsTranslation productsInitiator regionSecond codonCistronNonpermissive cellsSame proteinPhage RNAAUG tripletTandem promoters direct E. coli ribosomal RNA synthesis
Young R, Steitz J. Tandem promoters direct E. coli ribosomal RNA synthesis. Cell 1979, 17: 225-234. PMID: 110460, DOI: 10.1016/0092-8674(79)90310-6.Peer-Reviewed Original Research
1977
Characterization of two mRNA · rRNA complexes implicated in the initiation of protein biosynthesis
Steitz J, Steege D. Characterization of two mRNA · rRNA complexes implicated in the initiation of protein biosynthesis. Journal Of Molecular Biology 1977, 114: 545-558. PMID: 335077, DOI: 10.1016/0022-2836(77)90177-2.Peer-Reviewed Original ResearchConceptsProtein biosynthesisEscherichia coli 16 S ribosomal RNAS ribosomal RNABase pair regionNuclease digestion studiesFragment complexAssignment of residuesRibosomal RNARRNA complexLambda PRBacteriophage lambdaMolecular understandingInitiation siteInitiation eventsStrong experimental supportMessenger RNABiosynthesisThermal denaturation studiesDenaturation studiesRNAPR transcriptsPR regionDigestion studiesMRNAComplexesTwo ribosome binding sites from the gene 0.3 messenger RNA of bacteriophage T7
Steitz J, Bryan R. Two ribosome binding sites from the gene 0.3 messenger RNA of bacteriophage T7. Journal Of Molecular Biology 1977, 114: 527-543. PMID: 915941, DOI: 10.1016/0022-2836(77)90176-0.Peer-Reviewed Original ResearchConceptsGene 0.3 protein3′ Terminal sequences of 16S rRNA do not explain translational specificity differences between E. coli and B. stearothermophilus ribosomes
SPRAGUE KU, STEITZ JA, GRENLEY RM, STOCKING CE. 3′ Terminal sequences of 16S rRNA do not explain translational specificity differences between E. coli and B. stearothermophilus ribosomes. Nature 1977, 267: 462-465. PMID: 327330, DOI: 10.1038/267462a0.Peer-Reviewed Original ResearchDifferential requirements for polypeptide chain initiation complex formation at the three bacteriophage R17 initiator regions
Steitz J, Wahba A, Laughrea M, Moore P. Differential requirements for polypeptide chain initiation complex formation at the three bacteriophage R17 initiator regions. Nucleic Acids Research 1977, 4: 1-16. PMID: 325516, PMCID: PMC342405, DOI: 10.1093/nar/4.1.1.Peer-Reviewed Original Research
1975
F factor-mediated inhibition of bacteriophage T7 growth: Analysis of T7 RNA and protein synthesis In vivo and In vitro using male and female Escherichia coli
Condit RC, Steitz JA. F factor-mediated inhibition of bacteriophage T7 growth: Analysis of T7 RNA and protein synthesis In vivo and In vitro using male and female Escherichia coli. Journal Of Molecular Biology 1975, 98: 31-43. PMID: 1104867, DOI: 10.1016/s0022-2836(75)80099-4.Peer-Reviewed Original ResearchConceptsT7 growthProtein synthesisE. coliPolyacrylamide gel electrophoresisEscherichia coliPhage protein synthesisGel electrophoresisTranslation apparatusT7 proteinsPhage proteinsT7 infectionT7 DNAT7 RNAFaithful synthesisRNA synthesisProteinMale cellsRNAColiTranscriptionSimultaneous inhibitionSpecific modificationsT7InhibitionElectrophoresis
1974
Nucleotide sequences of the 5′ and 3′ termini of bacteriophage T7 early messenger RNAs synthesized in vivo: Evidence for sequence specificity in RNA processing
Kramer R, Rosenberg M, Steitz J. Nucleotide sequences of the 5′ and 3′ termini of bacteriophage T7 early messenger RNAs synthesized in vivo: Evidence for sequence specificity in RNA processing. Journal Of Molecular Biology 1974, 89: 767-776. PMID: 4449132, DOI: 10.1016/0022-2836(74)90051-5.Peer-Reviewed Original ResearchConceptsEarly regionMessenger RNAAdenylic acid residuesEarly messenger RNATwo-dimensional electrophoretic techniquesLarger precursor moleculeMonocistronic messengerRNA processingGene 0.3RNA speciesNucleotide sequenceSequence specificityAcid residuesMessenger speciesSequence analysisInitiator RNASequence heterogeneityRNAEarly RNATerminusT7 Early messenger RNAs are the direct products of ribonuclease III cleavage
Rosenberg M, Kramer R, Steitz J. T7 Early messenger RNAs are the direct products of ribonuclease III cleavage. Journal Of Molecular Biology 1974, 89: 777-782. PMID: 4615167, DOI: 10.1016/0022-2836(74)90052-7.Peer-Reviewed Original ResearchCistron specificity of 30S ribosomes heterologously reconstituted with components from Escherichia coli and Bacillus stearothermophilus.
Goldberg M, Steitz J. Cistron specificity of 30S ribosomes heterologously reconstituted with components from Escherichia coli and Bacillus stearothermophilus. Biochemistry 1974, 13: 2123-9. PMID: 4597073, DOI: 10.1021/bi00707a020.Peer-Reviewed Original ResearchDirect physical evidence for secondary structure in an isolated fragment of R17 bacteriophage mRNA
Gralla J, Steitz J, Crothers D. Direct physical evidence for secondary structure in an isolated fragment of R17 bacteriophage mRNA. Nature 1974, 248: 204-208. PMID: 4819414, DOI: 10.1038/248204a0.Peer-Reviewed Original ResearchSpecific recognition of the isolated R17 replicase initiator region by R17 coat protein
STEITZ J. Specific recognition of the isolated R17 replicase initiator region by R17 coat protein. Nature 1974, 248: 223-225. PMID: 4819415, DOI: 10.1038/248223a0.Peer-Reviewed Original ResearchHigh resolution proton NMR study of an isolated fragment of R17 bacteriophage mRNA
HILBERS CW, SHULMAN RG, YAMANE T, STEITZ JA. High resolution proton NMR study of an isolated fragment of R17 bacteriophage mRNA. Nature 1974, 248: 225-226. PMID: 4819416, DOI: 10.1038/248225a0.Peer-Reviewed Original ResearchConceptsHelical regionBase pairsBase pairingWatson-Crick base pairingIsolated fragmentsSecondary structureNMR spectraModel systemNuclear magnetic resonance spectraHigh-resolution nuclear magnetic resonance spectraNucleic acidsHydrogen-bonded protonsMagnetic resonance spectraProton NMR studiesAdjacent basesLeaf modelFragmentsNH protonsNMR studiesTRNAResonance spectraHigh-resolution proton NMR studiesHelixPairingGuanine
1973
Correction for non-uniform phosphate labeling of E. coli and phage RNAs synthesized in vivo
Kramer R, Steitz J. Correction for non-uniform phosphate labeling of E. coli and phage RNAs synthesized in vivo. Biochemical And Biophysical Research Communications 1973, 54: 1198-1202. PMID: 4584881, DOI: 10.1016/0006-291x(73)90819-x.Peer-Reviewed Original ResearchDiscriminatory Ribosome Rebinding of Isolated Regions of Protein Synthesis Initiation from the Ribonucleic Acid of Bacteriophage R17
Steitz J. Discriminatory Ribosome Rebinding of Isolated Regions of Protein Synthesis Initiation from the Ribonucleic Acid of Bacteriophage R17. Proceedings Of The National Academy Of Sciences Of The United States Of America 1973, 70: 2605-2609. PMID: 4582190, PMCID: PMC427065, DOI: 10.1073/pnas.70.9.2605.Peer-Reviewed Original ResearchConceptsR17 RNARibosome-binding siteProtein synthesis initiationEscherichia coli ribosomesPolypeptide chain initiationCoat protein regionRibosome bindingBacterial ribosomeRNA moleculesInitiator regionRibosome protectionSynthesis initiationMRNA moleculesInitiator codonColi ribosomesRibosomesNative RNABacteriophage R17Bacillus stearothermophilusRNAMessenger RNARibonucleic acidR17Replicase regionFragmentsSpecific recognition of non-initiator regions in RNA bacteriophage messengers by ribosomes of Bacillus stearothermophilus
Steitz J. Specific recognition of non-initiator regions in RNA bacteriophage messengers by ribosomes of Bacillus stearothermophilus. Journal Of Molecular Biology 1973, 73: 1-16. PMID: 4570382, DOI: 10.1016/0022-2836(73)90155-1.Peer-Reviewed Original ResearchBacillusBase SequenceBinding SitesCentrifugation, Density GradientChromatography, Thin LayerColiphagesDipeptidesEscherichia coliGenetic CodeNucleotidesPeptide Chain Initiation, TranslationalPeptide Initiation FactorsRibonucleasesRibosomesRNA, MessengerRNA, TransferRNA, ViralSpecies SpecificityTemperatureViral Proteins
1970
Control of Translation by T4 Phage : Altered Ribosome Binding at R17 Initiation Sites
STEITZ J, DUBE S, RUDLAND P. Control of Translation by T4 Phage : Altered Ribosome Binding at R17 Initiation Sites. Nature 1970, 226: 824-827. PMID: 5444624, DOI: 10.1038/226824a0.Peer-Reviewed Original Research
1969
Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA
STEITZ J. Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA. Nature 1969, 224: 957-964. PMID: 5360547, DOI: 10.1038/224957a0.Peer-Reviewed Original Research