2019
The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato
Coumou J, Wagemakers A, Narasimhan S, Schuijt TJ, Ersoz JI, Oei A, de Boer OJ, Roelofs JJTH, Fikrig E, Hovius JW. The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato. Scientific Reports 2019, 9: 1431. PMID: 30723261, PMCID: PMC6363739, DOI: 10.1038/s41598-018-37922-8.Peer-Reviewed Original ResearchConceptsMannose-Binding LectinB. burgdorferiImmune responseComplement systemRole of MBLMBL-deficient miceWhole blood stimulationIgG serum antibodiesB. burgdorferi infectionB. burgdorferi numbersHost complement systemMechanism warrants further investigationSerum-sensitive isolatesBorrelia burgdorferi sensu lato groupWarrants further investigationBorrelia burgdorferi sensu latoLater time pointsBinding lectinsSevere courseBlood stimulationDetectable antibodiesBurgdorferi sensu latoSerum antibodiesMBL deficiencyDeficient mice
2012
Immunization with adenoviral-vectored tick salivary gland proteins (SALPs) in a murine model of Lyme borreliosis
Ullmann AJ, Dolan MC, Sackal CA, Fikrig E, Piesman J, Zeidner NS. Immunization with adenoviral-vectored tick salivary gland proteins (SALPs) in a murine model of Lyme borreliosis. Ticks And Tick-borne Diseases 2012, 4: 160-163. PMID: 23141105, PMCID: PMC4306421, DOI: 10.1016/j.ttbdis.2012.08.006.Peer-Reviewed Original ResearchConceptsTick salivary proteinsBorrelia burgdorferi infectionSalivary proteinsTick salivary gland proteinsSalivary gland proteinsTh1 responseImmunized miceSpecific immunityBurgdorferi infectionMurine modelSpirochete loadLyme borreliosisPrior exposureAdenovirus expression systemAdenoviral vectorTick challengeVaccinationMammalian hostsIxodes scapularisVertebrate hosts
2009
The Urokinase Receptor (uPAR) Facilitates Clearance of Borrelia burgdorferi
Hovius JW, Bijlsma MF, van der Windt GJ, Wiersinga WJ, Boukens BJ, Coumou J, Oei A, de Beer R, de Vos AF, van 't Veer C, van Dam AP, Wang P, Fikrig E, Levi MM, Roelofs JJ, van der Poll T. The Urokinase Receptor (uPAR) Facilitates Clearance of Borrelia burgdorferi. PLOS Pathogens 2009, 5: e1000447. PMID: 19461880, PMCID: PMC2678258, DOI: 10.1371/journal.ppat.1000447.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArthritis, InfectiousBorrelia burgdorferiCell MovementHeartHistocytochemistryHumansLeukocytesLyme DiseaseMiceMice, Inbred C57BLMice, KnockoutMyocarditisPhagocytosisReceptors, Urokinase Plasminogen ActivatorSkinStatistics, NonparametricUp-RegulationUrinary BladderUrokinase-Type Plasminogen ActivatorConceptsB. burgdorferi numbersWT controlsPhagocytotic capacityC3H/HeN backgroundIL-1beta mRNA expressionBorrelia burgdorferiB. burgdorferi infectionRole of uPARSevere carditisBurgdorferi infectionImmune responseLeukocyte functionSpirochete Borrelia burgdorferiFibrinolytic systemPAI-1Facilitate clearanceMRNA expressionHuman leukocytesLyme borreliosisMiceB. burgdorferiCausative agentProteinase receptorUPARAdequate eradication
2004
Borrelia burgdorferi Changes Its Surface Antigenic Expression in Response to Host Immune Responses
Liang FT, Yan J, Mbow ML, Sviat SL, Gilmore RD, Mamula M, Fikrig E. Borrelia burgdorferi Changes Its Surface Antigenic Expression in Response to Host Immune Responses. Infection And Immunity 2004, 72: 5759-5767. PMID: 15385475, PMCID: PMC517580, DOI: 10.1128/iai.72.10.5759-5767.2004.Peer-Reviewed Original ResearchConceptsSurface antigenic expressionAntigenic expressionImmune attackImmune responseSevere combined immunodeficiency miceHostile immune environmentVigorous immune responseB. burgdorferiCombined immunodeficiency miceHost immune responseOspC antibodiesOuter surface protein CImmune environmentDecorin-binding protein AImmunocompetent miceImmune conditionsImmunodeficiency miceSurface protein CImmune pressureVlsE expressionLyme disease spirocheteProtein CBorrelia burgdorferiOspC expressionMammalian infectionProtective Niche for Borrelia burgdorferi to Evade Humoral Immunity
Liang FT, Brown EL, Wang T, Iozzo RV, Fikrig E. Protective Niche for Borrelia burgdorferi to Evade Humoral Immunity. American Journal Of Pathology 2004, 165: 977-985. PMID: 15331421, PMCID: PMC1618599, DOI: 10.1016/s0002-9440(10)63359-7.Peer-Reviewed Original ResearchConceptsChronic infectionHumoral immunityProtective nicheB. burgdorferiDecorin expressionLower decorin expressionDecorin-deficient miceWild-type miceStrong immune responseDecorin-binding protein ASpirochete burdenUrinary bladderImmune responsePersistent infectionMurine infectionEarly infectionInfectionExtracellular microbesDbpA expressionLyme disease spirocheteBorrelia burgdorferiC expressionTargeted disruptionBurgdorferiMice
2003
Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
Narasimhan S, Camaino M, Liang FT, Santiago F, Laskowski M, Philipp MT, Pachner AR, Radolf JD, Fikrig E. Borrelia burgdorferi transcriptome in the central nervous system of non-human primates. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 15953-15958. PMID: 14671329, PMCID: PMC307674, DOI: 10.1073/pnas.2432412100.Peer-Reviewed Original ResearchConceptsNon-human primate modelB. burgdorferiEffect of dexamethasoneCentral nervous systemHost metabolic pathwaysNon-human primatesNeurological symptomsPaucibacillary natureCNS milieuImmune statusCommon manifestationPrimate modelNervous systemExpression profilesLyme diseaseHost factorsHeart tissueGene expressionB. burgdorferi transcriptomeBorrelia burgdorferiNHPNeuroborreliosisImmunocompetentVivo gene expressionBurgdorferi