2000
Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Padula SJ, Fikrig E. Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States. Journal Of The American Veterinary Medical Association 2000, 217: 1045-50. PMID: 11019714, DOI: 10.2460/javma.2000.217.1045.Peer-Reviewed Original ResearchConceptsB burgdorferiClinical signsGranulocytic ehrlichiosisSerum samplesDetectable serum antibodiesImmunoblot analysisIndirect fluorescent antibody stainingBorrelial antibodiesTick-infested areasRecombinant protein antigensProspective studyDetectable antibodiesSerum antibodiesSerologic confirmationFluorescent antibody stainingE equiAntibody reactivityClinical relevanceEtiologic agentClinical diagnosisEqui antibodiesProtein antigensEhrlichia equiELISAAntibodiesSerologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens
Magnarelli L, Ijdo J, Padula S, Flavell R, Fikrig E. Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens. Journal Of Clinical Microbiology 2000, 38: 1735-1739. PMID: 10790090, PMCID: PMC86574, DOI: 10.1128/jcm.38.5.1735-1739.2000.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayHuman granulocytic ehrlichiosisRecombinant antigensClass-specific enzyme-linked immunosorbent assaysIgG antibodiesAntibody reactivityLyme borreliosisSerum samplesImmunoblotting of seraImmunoglobulin M antibodiesLow antibody titersB. burgdorferiEnzyme-linked immunosorbentWhole cell antigenAnalysis of serumWestern blot analysisOuter surface protein CErythema migransRheumatoid arthritisAntibody titersIgM antibodiesM antibodiesBorrelia burgdorferi sensu strictoOral infectionSurface protein C
1994
Sera from Patients with Chronic Lyme Disease Protect Mice from Lyme Borreliosis
Fikrig E, Bockenstedt L, Barthold S, Chen M, Tao H, Salaam P, Telford S, Flavell R. Sera from Patients with Chronic Lyme Disease Protect Mice from Lyme Borreliosis. The Journal Of Infectious Diseases 1994, 169: 568-574. PMID: 8158028, DOI: 10.1093/infdis/169.3.568.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupChronic DiseaseFemaleHumansImmunization, PassiveImmunoblottingLipoproteinsLyme DiseaseMiceMice, Inbred C3HPolymerase Chain ReactionPrecipitin TestsConceptsStrong humoral responseLyme diseaseHumoral responseB. burgdorferiLate-stage Lyme diseaseLate Lyme diseaseElicit protective immunityChronic Lyme diseaseStrong antibody reactivityBorrelia burgdorferi challengeSurface protein AMost patientsOuter surface protein AProtective immunityImmune responsePatientsAntibody reactivityHuman antibodiesInfectionLyme borreliosisMiceDiseaseSerumNatural infectionAntibodies