Artificial membrane-like environments for in vitro studies of purified G-protein coupled receptors
Serebryany E, Zhu GA, Yan EC. Artificial membrane-like environments for in vitro studies of purified G-protein coupled receptors. Biochimica Et Biophysica Acta 2011, 1818: 225-233. PMID: 21851807, DOI: 10.1016/j.bbamem.2011.07.047.Peer-Reviewed Original ResearchConceptsFunctional reconstitutionG proteinsMembrane protein structuresNative-like environmentSeven-transmembrane G-proteinMembrane-like environmentTransmembrane proteinLipidic cubic phaseCell signalingPlanar lipid membranesProtein structureMembrane mimicsMolecular levelGPCR researchLipid vesiclesLipid membranesMembraneReceptorsReconstitutionUse of micellesGPCRsSignalingNanodiscsProteinVesiclesChiral Sum Frequency Generation Spectroscopy for Characterizing Protein Secondary Structures at Interfaces
Fu L, Liu J, Yan EC. Chiral Sum Frequency Generation Spectroscopy for Characterizing Protein Secondary Structures at Interfaces. Journal Of The American Chemical Society 2011, 133: 8094-8097. PMID: 21534603, DOI: 10.1021/ja201575e.Peer-Reviewed Original ResearchConceptsChiral sum frequency generation (SFG) spectroscopySum frequency generation spectroscopyFrequency generation spectroscopyProtein secondary structureVibrational signaturesGeneration spectroscopyChiral SFG spectroscopyChiral SFG spectraRandom coilSecondary structureΑ-helixΒ-sheetLipid-water interfaceSFG spectroscopyHuman islet amyloidPeptide backboneSFG spectraH stretchAmide IReal-time characterizationSpectroscopyProtein conformationIslet amyloidStructureSitu