Complex developmental patterns of histone modifications associated with the human β-globin switch in primary cells
Hsu M, Richardson CA, Olivier E, Qiu C, Bouhassira EE, Lowrey CH, Fiering S. Complex developmental patterns of histone modifications associated with the human β-globin switch in primary cells. Experimental Hematology 2009, 37: 799-806.e4. PMID: 19460472, PMCID: PMC2748252, DOI: 10.1016/j.exphem.2009.04.006.Peer-Reviewed Original ResearchConceptsHistone modificationsGene expressionBeta-globin switchDimethyl lysine 9Beta-globin locusΒ-globin switchGamma geneImportant histone modificationsBone marrow cellsGamma gene expressionSuch histonesComplex developmental patternLysine 9Chromatin immunoprecipitationHistone H3Gene suppressionPrimary fetalMarrow cellsUnexpressed genesGamma-globinGenesHistonesDevelopmental stagesBone marrow erythroblastsPrimary cellsDevelopmentally regulated extended domains of DNA hypomethylation encompass highly transcribed genes of the human β-globin locus
Lathrop MJ, Hsu M, Richardson CA, Olivier EN, Qiu C, Bouhassira EE, Fiering S, Lowrey CH. Developmentally regulated extended domains of DNA hypomethylation encompass highly transcribed genes of the human β-globin locus. Experimental Hematology 2009, 37: 807-813.e2. PMID: 19460471, PMCID: PMC3792488, DOI: 10.1016/j.exphem.2009.04.005.Peer-Reviewed Original ResearchConceptsAdult erythroid cellsCpG island genesHistone modificationsGene expressionDNA hypomethylationErythroid cellsDNA methylationBeta-like globin gene expressionBeta-globin gene regulationIsland genesActive histone marksBeta-globin locusGamma-globin promoterGlobin gene expressionHuman embryonic stem cellsDNA methylation patternsSpecific gene expressionEmbryonic stem cellsPrimary human erythroblastsHuman β-globinHistone marksGene regulationMethylation stateBisulfite sequencingCpG islands