2020
Cytoskeletal Drugs Modulate Off-Target Protein Folding Landscapes Inside Cells
Davis CM, Gruebele M. Cytoskeletal Drugs Modulate Off-Target Protein Folding Landscapes Inside Cells. Biochemistry 2020, 59: 2650-2659. PMID: 32567840, DOI: 10.1021/acs.biochem.0c00299.Peer-Reviewed Original ResearchConceptsCytoskeletal drugsPhosphoglycerate kinaseActin filamentsDynamic cytoskeletal networksEffects of cytoskeletonProtein energy landscapesOff-target proteinsOpposite responseCytoskeletal networkProtein stabilityCellular milieuProtein-like sequencesVariable major protein-like sequenceOverall cell volumeCytoskeletonCell migrationEnergy landscapeMacromolecular crowdingMacromolecular crowdersProteinNonspecific surface interactionsTarget effectsMicrotubulesCytoplasmCells
2018
Non‐Steric Interactions Predict the Trend and Steric Interactions the Offset of Protein Stability in Cells
Davis CM, Gruebele M. Non‐Steric Interactions Predict the Trend and Steric Interactions the Offset of Protein Stability in Cells. ChemPhysChem 2018, 19: 2290-2294. PMID: 29877016, DOI: 10.1002/cphc.201800534.Peer-Reviewed Original ResearchConceptsProtein foldingProtein stabilityNon-steric interactionsSteric interactionsEukaryotic cellsProtein flexibilityIntracellular environmentBiochemical assaysCell lysatesPGKCells resultsSolvation environmentFoldingChemical behaviorLysis bufferGreater stabilizationStability trendCellsCell studiesVlsESystematic seriesIonic bufferEffective newsProteinInteraction