2018
Soluble Zwitterionic Poly(sulfobetaine) Destabilizes Proteins
Kisley L, Miller K, Davis CM, Guin D, Murphy EA, Gruebele M, Leckband DE. Soluble Zwitterionic Poly(sulfobetaine) Destabilizes Proteins. Biomacromolecules 2018, 19: 3894-3901. PMID: 30064224, DOI: 10.1021/acs.biomac.8b01120.Peer-Reviewed Original ResearchConceptsProtein melting temperatureBiotechnological applicationsSpecific proteinsProtein conformationTryptophan fluorescenceProteinTryptophan residuesThermal denaturation studiesDenaturation studiesCooperativity increasesProtein bindingPSBLocal polarityInteractsResiduesPEG solutionBindingCooperativityConformationFluorescence
2017
Dual time-resolved temperature-jump fluorescence and infrared spectroscopy for the study of fast protein dynamics
Davis CM, Reddish MJ, Dyer RB. Dual time-resolved temperature-jump fluorescence and infrared spectroscopy for the study of fast protein dynamics. Spectrochimica Acta Part A Molecular And Biomolecular Spectroscopy 2017, 178: 185-191. PMID: 28189834, PMCID: PMC5346054, DOI: 10.1016/j.saa.2017.01.069.Peer-Reviewed Original ResearchConceptsQuantum cascade lasersTime-resolved IRYAG laserTunable quantum cascade laserFluorescence spectroscopyProtein dynamicsAbsorbance detection limitCascade lasersSapphire laserComplex folding mechanismsLaserIR frequenciesOverall fluorescence intensityFluorescence spectrometerSpectroscopyIR spectrometerFluorescence measurementsFluorescence sensitivityHigh sensitivityT-jumpSpectrometerFast protein dynamicsFolding mechanismPowerful techniqueFluorescence