2023
Gene and protein expression and metabolic flux analysis reveals metabolic scaling in liver ex vivo and in vivo
Akingbesote N, Leitner B, Jovin D, Desrouleaux R, Owusu D, Zhu W, Li Z, Pollak M, Perry R. Gene and protein expression and metabolic flux analysis reveals metabolic scaling in liver ex vivo and in vivo. ELife 2023, 12: e78335. PMID: 37219930, PMCID: PMC10205083, DOI: 10.7554/elife.78335.Peer-Reviewed Original ResearchConceptsMetabolic scalingMetabolic fluxMetabolic processesBody sizeMitochondrial metabolic processesMultiple cellular compartmentsVivo metabolic fluxesLevel of genesKey metabolic pathwaysProtein expressionMetabolic flux analysisAspects of metabolismCellular compartmentsGene expressionDifferential expressionMetabolic pathwaysGenesFlux analysisOxidative damageEnzyme activitySubstrate supplyFold rangeSpeciesExpressionMetabolic rate
2022
An optimized method for tissue glycogen quantification
Schaubroeck KJ, Leitner BP, Perry RJ. An optimized method for tissue glycogen quantification. Physiological Reports 2022, 10: e15195. PMID: 35179318, PMCID: PMC8855679, DOI: 10.14814/phy2.15195.Peer-Reviewed Original Research
2021
1-13C-propionate breath testing as a surrogate endpoint to assess efficacy of liver-directed therapies in methylmalonic acidemia (MMA)
Manoli I, Pass A, Harrington E, Sloan J, Gagné J, McCoy S, Bell S, Hattenbach J, Leitner B, Duckworth C, Fletcher L, Cassimatis T, Galarreta C, Thurm A, Snow J, Van Ryzin C, Ferry S, Mew N, Shchelochkov O, Chen K, Venditti C. 1-13C-propionate breath testing as a surrogate endpoint to assess efficacy of liver-directed therapies in methylmalonic acidemia (MMA). Genetics In Medicine 2021, 23: 1522-1533. PMID: 33820958, PMCID: PMC8354855, DOI: 10.1038/s41436-021-01143-8.Peer-Reviewed Original ResearchConceptsMethylmalonic acidemiaTransplant recipientsBreath testingSerum methylmalonic acid concentrationsLiver transplant recipientsLiver-directed therapiesBone mineral densityNatural history protocolMethylmalonic acid concentrationsOxidative capacityEnteral bolusRenal functionClinical outcomesCognitive test resultsSerum biomarkersMineral densityClinical studiesTherapeutic effectBreath testHealthy volunteersSurrogate endpointsDisease severityRelated disordersGenomic therapyPatients