Implications of macromolecular crowding for signal transduction and metabolite channeling
Rohwer J, Postma P, Kholodenko B, Westerhoff H. Implications of macromolecular crowding for signal transduction and metabolite channeling. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10547-10552. PMID: 9724740, PMCID: PMC27931, DOI: 10.1073/pnas.95.18.10547.Peer-Reviewed Original ResearchMeSH KeywordsCell-Free SystemEscherichia coliKineticsModels, BiologicalPhosphoenolpyruvate Sugar Phosphotransferase SystemSignal TransductionConceptsPhosphotransferase systemMacromolecular crowdingFlux response coefficientsCarbohydrate phosphotransferase systemGlucose phosphotransferase systemBacterial phosphoenolpyruvateSignal transductionMetabolite channelingCell-free extractsProtein concentrationEnzyme complexCarbohydrate phosphorylationPEG 6000 concentrationPolyethylene glycol 6000Total enzyme concentrationHigh protein concentrationsEscherichia coliLow protein concentrationsForm complexesGroup-transfer pathwaysMetabolic behaviorPEG 6000Crowded environments