2017
Randomized, Controlled Trial of TRC101 to Increase Serum Bicarbonate in Patients with CKD
Bushinsky DA, Hostetter T, Klaerner G, Stasiv Y, Lockey C, McNulty S, Lee A, Parsell D, Mathur V, Li E, Buysse J, Alpern R. Randomized, Controlled Trial of TRC101 to Increase Serum Bicarbonate in Patients with CKD. Clinical Journal Of The American Society Of Nephrology 2017, 13: 26-35. PMID: 29102959, PMCID: PMC5753317, DOI: 10.2215/cjn.07300717.Peer-Reviewed Original ResearchConceptsSerum bicarbonateEnd of treatmentMetabolic acidosisTreatment groupsBaseline serum bicarbonateMean baseline eGFRMEq/LSignificant adverse effectsBaseline eGFRGastrointestinal eventsAdverse eventsFirst doseControlled TrialsHeart failurePatientsCKDBaseline levelsMean increaseAbstractTextUnit studiesAcidosisAdverse effectsPlaceboTreatmentDays
2004
OKP cells express the Na-dicarboxylate cotransporter NaDC-1
Aruga S, Pajor AM, Nakamura K, Liu L, Moe OW, Preisig PA, Alpern RJ. OKP cells express the Na-dicarboxylate cotransporter NaDC-1. American Journal Of Physiology - Cell Physiology 2004, 287: c64-c72. PMID: 14973148, DOI: 10.1152/ajpcell.00061.2003.Peer-Reviewed Original ResearchConceptsCitrate reabsorptionOKP cellsProximal tubulesDependent citrate uptakeOpossum kidney cell lineUrinary citrate concentrationChronic metabolic acidosisRate of reabsorptionRenal proximal tubulesMetabolic acidosisStone formationKidney stonesKidney cell lineCotransporter 1ReabsorptionCell linesCitrate transportMRNA abundanceXenopus oocytesAcid regulationTubulesNaDC-1Citrate uptakeSuccinate transportHypocitraturia
2002
Glucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells
Amemiya M, Kusano E, Muto S, Tabei K, Ando Y, Alpern RJ, Asano Y. Glucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells. Experimental Nephrology 2002, 10: 26-33. PMID: 11803202, DOI: 10.1159/000049895.Peer-Reviewed Original ResearchConceptsNHE3 protein abundanceOKP cellsNHE3 activityCell pH recoveryRat renal proximal tubulesDibutyryl cAMPThick ascending limbRenal proximal tubulesWestern blot analysisM dibutyryl-cAMPChronic activationPKA-dependent pathwayProximal tubulesAscending limbGlucagonSurface membraneAcid loadNHE3 mRNAProtein abundancePH recoveryExchanger 3Independent mechanismsSignificant increaseBlot analysisP cells
1998
Converting enzyme inhibition causes hypocitraturia independent of acidosis or hypokalemia
Melnick J, Preisig P, Haynes S, Pak C, Sakhaee K, Alpern R. Converting enzyme inhibition causes hypocitraturia independent of acidosis or hypokalemia. Kidney International 1998, 54: 1670-1674. PMID: 9844143, DOI: 10.1046/j.1523-1755.1998.00150.x.Peer-Reviewed Original ResearchConceptsUrinary citrate excretionCitrate excretionUrinary citrateMetabolic acidosisATP-citrate lyaseEffects of enalaprilRenal citrate metabolismUrine citrate concentrationRenal cortical tissueChronic metabolic acidosisProximal tubular cellsPlasma pHNa/H antiporterAngiotensin IIIntracellular acidosisEnalaprilTubular cellsCitrate lyaseHypokalemiaEnzyme inhibitorsATP citrate lyase activityAcidosisCortical tissueRatsCitrate metabolismEffects of thyroid hormone on the neonatal renal cortical Na+/H+ antiporter
Baum M, Dwarakanath V, Alpern R, Moe O. Effects of thyroid hormone on the neonatal renal cortical Na+/H+ antiporter. Kidney International 1998, 53: 1254-1258. PMID: 9573540, PMCID: PMC4131927, DOI: 10.1046/j.1523-1755.1998.00879.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAntithyroid AgentsFemaleHyperthyroidismHypothyroidismKidney CortexKidney Tubules, ProximalMaternal-Fetal ExchangePregnancyPropylthiouracilRatsRats, Sprague-DawleyRNA, MessengerSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSodium-Potassium-Exchanging ATPaseTriiodothyronineConceptsThyroid hormonesHyperthyroid neonatesNHE-3 protein abundanceNHE-3 mRNA abundanceDays of lifeLower ratesNeonatal proximal tubuleAntiporter activityHypothyroid neonatesHyperthyroid groupMaturational increaseProximal tubulesPostnatal increaseHyperthyroid ratsBicarbonate absorptionPmol/NeonatesHormonePotential roleBrush borderEuthyroidOne-thirdRatsMRNA abundanceApical membraneLiddle’s Syndrome
Palmer B, Alpern R, In collaboration with The American Physiological Society T. Liddle’s Syndrome. The American Journal Of Medicine 1998, 104: 301-309. PMID: 9552093, DOI: 10.1016/s0002-9343(98)00018-7.Peer-Reviewed Original Research
1994
Effect of glucocorticoids on renal cortical NHE-3 and NHE-1 mRNA
Baum M, Moe OW, Gentry DL, Alpern RJ. Effect of glucocorticoids on renal cortical NHE-3 and NHE-1 mRNA. American Journal Of Physiology 1994, 267: f437-f442. PMID: 8092257, DOI: 10.1152/ajprenal.1994.267.3.f437.Peer-Reviewed Original ResearchConceptsNHE-3 mRNA abundanceProximal tubulesNHE-3NHE-1MRNA abundanceChronic systemic administrationAdministration of dexamethasoneEffects of glucocorticoidsProximal tubule acidificationNHE-1 mRNAUnits/minChronic dexamethasoneTubule acidificationAcute increaseAntiporter activitySystemic administrationGlucocorticoid deficiencyIntravenous administrationBicarbonate absorptionMicrograms/DexamethasoneAntiporter isoformsDexamethasone occursRenal brush-border membrane vesiclesAdministration
1992
Citraturic Response to Oral Citric Acid Load
Sakhaee K, Alpern R, Poindexter J, Pak C. Citraturic Response to Oral Citric Acid Load. Journal Of Urology 1992, 147: 975-976. PMID: 1552616, DOI: 10.1016/s0022-5347(17)37437-2.Peer-Reviewed Original ResearchConceptsAcid loadCitraturic responseUrinary citrateCarbon dioxide pressureSingle oral loadUrinary citrate excretionSingle oral doseSerum citrate concentrationAcid-base balanceCitraturic effectsOral loadVenous pHOral doseSerum electrolytesUrinary pHCitrate excretionNormal subjectsAlkali loadPotassium citrateSignificant differencesHoursTotal carbon dioxideCitrateExcretionCitrate concentration
1991
Contrasting Effects of Various Potassium Salts on Renal Citrate Excretion*
SAKHAEE K, ALPERN R, JACOBSON H, PAK C. Contrasting Effects of Various Potassium Salts on Renal Citrate Excretion*. The Journal Of Clinical Endocrinology & Metabolism 1991, 72: 396-400. PMID: 1899422, DOI: 10.1210/jcem-72-2-396.Peer-Reviewed Original ResearchConceptsCitrate clearanceAcid-base statusUrinary citratePotassium citrateRenal citrate excretionMeq/dayPotassium citrate treatmentWeeks of treatmentNet acid excretionCitraturic responseUrinary ammoniumOral administrationCitrate excretionAcid excretionPotassium chlorideUrinary bicarbonateCitrate treatmentAlkali loadPotassium bicarbonateClearanceML/minExcretionDaysTreatmentTitratable acid
1990
Apical and basolateral membrane H+ extrusion mechanisms in inner stripe of rabbit outer medullary collecting duct
Hays SR, Alpern RJ. Apical and basolateral membrane H+ extrusion mechanisms in inner stripe of rabbit outer medullary collecting duct. American Journal Of Physiology 1990, 259: f628-f635. PMID: 2171359, DOI: 10.1152/ajprenal.1990.259.4.f628.Peer-Reviewed Original ResearchConceptsUnits/minPHi recoveryInner stripeAcid loadInitial control levelsSCH 28080Luminal Sch-28080ATPase inhibitorBasolateral membraneTriphasic responseExtrusion mechanismSlow alkalinizationRate of alkalinizationControl levelsCell acidificationRabbitsInhibitorsDuctTotal absenceInitial rateAlkalinizationN-ethylmaleimideRecoveryMinBasolateral membrane Na(+)-independent Cl-/HCO3- exchange in the inner stripe of the rabbit outer medullary collecting tubule.
Hays SR, Alpern RJ. Basolateral membrane Na(+)-independent Cl-/HCO3- exchange in the inner stripe of the rabbit outer medullary collecting tubule. The Journal Of General Physiology 1990, 95: 347-367. PMID: 2307960, PMCID: PMC2216319, DOI: 10.1085/jgp.95.2.347.Peer-Reviewed Original ResearchConceptsInner stripePeritubular ClDistal nephron segmentsBasolateral membraneCell acidificationCell pHLuminal changesUrinary acidificationNephron segmentsPH-sensitive fluorescent dyeIndependent ClCell alkalinizationCO2/HCO3Peritubular pHVoltage clampTubulesTwo-thirdsRabbitsTotal replacementCell pH changes
1986
Electrogenic sodium/bicarbonate cotransport in rabbit renal cortical basolateral membrane vesicles.
Akiba T, Alpern RJ, Eveloff J, Calamina J, Warnock DG. Electrogenic sodium/bicarbonate cotransport in rabbit renal cortical basolateral membrane vesicles. Journal Of Clinical Investigation 1986, 78: 1472-1478. PMID: 3782468, PMCID: PMC423898, DOI: 10.1172/jci112738.Peer-Reviewed Original ResearchConceptsMembrane vesiclesBasolateral membrane vesiclesSodium uptakePotassium diffusion potentialBicarbonate cotransporterSaturable stimulationSodium/bicarbonate cotransporterVesiclesRabbit renal cortexProteinDisulfonic stilbeneBicarbonate transportPresence of bicarbonateUptakeKinetic analysisRenal cortex
1984
Increased Vmax for Na+/H+ antiporter activity in proximal tubule brush border vesicles from rabbits with metabolic acidosis
Tsai CJ, Ives HE, Alpern RJ, Yee VJ, Warnock DG, Rector FC. Increased Vmax for Na+/H+ antiporter activity in proximal tubule brush border vesicles from rabbits with metabolic acidosis. American Journal Of Physiology 1984, 247: f339-f343. PMID: 6087681, DOI: 10.1152/ajprenal.1984.247.2.f339.Peer-Reviewed Original Research