2001
Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis
Laghmani K, Preisig P, Moe O, Yanagisawa M, Alpern R. Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis. Journal Of Clinical Investigation 2001, 107: 1563-1569. PMID: 11413164, PMCID: PMC200190, DOI: 10.1172/jci11234.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmoniaAnimalsBicarbonatesChronic DiseaseCitric AcidCulture TechniquesEndothelin-1EndothelinsKidney Tubules, ProximalMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicProtein PrecursorsReceptor, Endothelin BReceptors, EndothelinRNA, MessengerSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsTg/tgAcid ingestionTitratable acid excretionMetabolic acidosisNHE3 activityAcid excretionReceptor-deficient miceEndothelin B receptorET-1 expressionChronic metabolic acidosisProximal tubule suspensionsAmmonium excretionM endothelinNa/H antiporterC57BL/6 miceApical membrane NHE3Renal epithelial cellsCultured renal epithelial cellsEndothelin-1/Tubule suspensionsMRNA expressionControl dietMiceExcretionEpithelial cellsETB receptor activation causes exocytic insertion of NHE3 in OKP cells
Peng Y, Amemiya M, Yang X, Fan L, Moe O, Yin H, Preisig P, Yanagisawa M, Alpern R. ETB receptor activation causes exocytic insertion of NHE3 in OKP cells. American Journal Of Physiology. Renal Physiology 2001, 280: f34-f42. PMID: 11133512, DOI: 10.1152/ajprenal.2001.280.1.f34.Peer-Reviewed Original ResearchConceptsEndothelin-1Sodium/hydrogen exchanger 3ET-1-induced increaseTotal cellular NHE3NHE3 activityNET traffickingTwo- to threefold increaseApical membrane NHE3OKP cellsMaximal effectReceptor activationClone PApical NHE3Exchanger 3NHE3Exocytic insertionThreefold increaseApical membraneCytochalasin D
1999
ETB receptor activation leads to activation and phosphorylation of NHE3
Peng Y, Moe OW, Chu T, Preisig PA, Yanagisawa M, Alpern RJ. ETB receptor activation leads to activation and phosphorylation of NHE3. American Journal Of Physiology 1999, 276: c938-c945. PMID: 10199826, DOI: 10.1152/ajpcell.1999.276.4.c938.Peer-Reviewed Original Research
1996
Role of tyrosine kinase pathways in ETB receptor activation of NHE3
Chu TS, Tsuganezawa H, Peng Y, Cano A, Yanagisawa M, Alpern RJ. Role of tyrosine kinase pathways in ETB receptor activation of NHE3. American Journal Of Physiology 1996, 271: c763-c771. PMID: 8843705, DOI: 10.1152/ajpcell.1996.271.3.c763.Peer-Reviewed Original ResearchConceptsET-1-induced tyrosine phosphorylationTyrosine kinase pathwayTyrosine phosphorylationKinase pathwayFocal adhesion proteinsIntegral membrane proteinsAntiporter activityClonal cell linesFocal adhesionsMembrane proteinsReceptor activationAdhesion proteinsImmunoprecipitation studiesPhosphorylationCytochalasin DET-1-induced increaseApical membraneDependent pathwayAntiporter activationProteinPaxillinP125FAKCell linesPhosphoproteinPathwayEndothelin(B) receptor activates NHE-3 by a Ca2+-dependent pathway in OKP cells.
Chu TS, Peng Y, Cano A, Yanagisawa M, Alpern RJ. Endothelin(B) receptor activates NHE-3 by a Ca2+-dependent pathway in OKP cells. Journal Of Clinical Investigation 1996, 97: 1454-1462. PMID: 8617878, PMCID: PMC507205, DOI: 10.1172/jci118567.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityReceptor blockersET-1OKP cellsET-1-induced increaseEndothelin receptor blockersNHE-3Pertussis toxin pretreatmentSelective receptor blockerAntiporter activationM endothelinPertussis toxinToxin pretreatmentSustained increaseAntiporter isoformsEndothelinTransient increaseAntiporter activityBlockersKinase inhibitorsCyclic GMPAdenylyl cyclaseReceptor cDNADependent pathwayReceptors