2000
Renal citrate metabolism and urinary citrate excretion in the infant rat
Melnick J, Preisig P, Alpern R, Baum M. Renal citrate metabolism and urinary citrate excretion in the infant rat. Kidney International 2000, 57: 891-897. PMID: 10720942, PMCID: PMC4089854, DOI: 10.1046/j.1523-1755.2000.057003891.x.Peer-Reviewed Original ResearchConceptsUrinary citrate excretionHigher urinary citrateCitrate excretionInfant ratsAdult ratsUrinary citrateRenal citrate metabolismMitochondrial aconitase activityCreatinine ratioCitrate metabolismRenal excretionSame prevalenceRenal stonesTwofold higher concentrationLower plasmaATP citrate lyase activityProximal tubulesHigher plasmaMetabolic cagesRat kidneyRatsAdult levelsInfantsMaturational differencesExcretion
1998
Converting enzyme inhibition causes hypocitraturia independent of acidosis or hypokalemia
Melnick J, Preisig P, Haynes S, Pak C, Sakhaee K, Alpern R. Converting enzyme inhibition causes hypocitraturia independent of acidosis or hypokalemia. Kidney International 1998, 54: 1670-1674. PMID: 9844143, DOI: 10.1046/j.1523-1755.1998.00150.x.Peer-Reviewed Original ResearchConceptsUrinary citrate excretionCitrate excretionUrinary citrateMetabolic acidosisATP-citrate lyaseEffects of enalaprilRenal citrate metabolismUrine citrate concentrationRenal cortical tissueChronic metabolic acidosisProximal tubular cellsPlasma pHNa/H antiporterAngiotensin IIIntracellular acidosisEnalaprilTubular cellsCitrate lyaseHypokalemiaEnzyme inhibitorsATP citrate lyase activityAcidosisCortical tissueRatsCitrate metabolismRenal Na/H exchanger NHE-3 and Na-PO4 cotransporter NaPi-2 protein expression in glucocorticoid excess and deficient states.
Loffing J, Lötscher M, Kaissling B, Biber J, Murer H, Seikaly M, Alpern RJ, Levi M, Baum M, Moe OW. Renal Na/H exchanger NHE-3 and Na-PO4 cotransporter NaPi-2 protein expression in glucocorticoid excess and deficient states. Journal Of The American Society Of Nephrology 1998, 9: 1560-7. PMID: 9727362, PMCID: PMC4131923, DOI: 10.1681/asn.v991560.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex 3Adaptor Protein Complex beta SubunitsAdrenalectomyAnalysis of VarianceAnimalsBiological Transport, ActiveCarrier ProteinsDisease Models, AnimalGlucocorticoidsImmunoblottingImmunohistochemistryKidney CortexMaleNerve Tissue ProteinsPhosphoproteinsRatsRats, Sprague-DawleySensitivity and SpecificitySodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSodium-Phosphate Cotransporter ProteinsSymportersConceptsNa/H exchangeNHE-3 protein abundanceNHE-3Glucocorticoid excessPharmacologic dosesNaPi-2 proteinProximal tubulesSham-operated ratsNaPi-2Sham-operated animalsThick ascending limbSignificant reciprocal increaseBilateral adrenalectomyDexamethasone administrationIntrarenal distributionControl ratsProtein abundanceAscending limbReciprocal increaseAdrenalectomyRatsDeficient stateCotransport activityProtein expressionAdministrationEffects of thyroid hormone on the neonatal renal cortical Na+/H+ antiporter
Baum M, Dwarakanath V, Alpern R, Moe O. Effects of thyroid hormone on the neonatal renal cortical Na+/H+ antiporter. Kidney International 1998, 53: 1254-1258. PMID: 9573540, PMCID: PMC4131927, DOI: 10.1046/j.1523-1755.1998.00879.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAntithyroid AgentsFemaleHyperthyroidismHypothyroidismKidney CortexKidney Tubules, ProximalMaternal-Fetal ExchangePregnancyPropylthiouracilRatsRats, Sprague-DawleyRNA, MessengerSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSodium-Potassium-Exchanging ATPaseTriiodothyronineConceptsThyroid hormonesHyperthyroid neonatesNHE-3 protein abundanceNHE-3 mRNA abundanceDays of lifeLower ratesNeonatal proximal tubuleAntiporter activityHypothyroid neonatesHyperthyroid groupMaturational increaseProximal tubulesPostnatal increaseHyperthyroid ratsBicarbonate absorptionPmol/NeonatesHormonePotential roleBrush borderEuthyroidOne-thirdRatsMRNA abundanceApical membrane
1997
Adaptation to low-K+ media increases H(+)-K(+)-ATPase but not H(+)-ATPase-mediated pHi recovery in OMCD1 cells
Guntupalli J, Onuigbo M, Wall S, Alpern RJ, DuBose TD. Adaptation to low-K+ media increases H(+)-K(+)-ATPase but not H(+)-ATPase-mediated pHi recovery in OMCD1 cells. American Journal Of Physiology 1997, 273: c558-c571. PMID: 9277353, DOI: 10.1152/ajpcell.1997.273.2.c558.Peer-Reviewed Original Research
1996
Adenosine triphosphate citrate lyase mediates hypocitraturia in rats.
Melnick JZ, Srere PA, Elshourbagy NA, Moe OW, Preisig PA, Alpern RJ. Adenosine triphosphate citrate lyase mediates hypocitraturia in rats. Journal Of Clinical Investigation 1996, 98: 2381-2387. PMID: 8941657, PMCID: PMC507690, DOI: 10.1172/jci119051.Peer-Reviewed Original ResearchConceptsChronic metabolic acidosisMetabolic acidosisATP citrate lyase activityATP-citrate lyaseCitrate lyase activityCitrate lyaseHypocitraturiaAcid dietAcidosisCitrate metabolismProtein abundanceRatsMRNA abundanceLyase activityMetabolismPresent studySignificant changesCitrate uptakeInhibitionHypercitraturiaHypokalemiaCytosolic enzymeCompetitive inhibitorActivityImportant roleChronic metabolic acidosis increases NHE3 protein abundance in rat kidney
Ambuhl P, Amemiya M, Danczkay M, Lotscher M, Kaissling B, Moe OW, Preisig PA, Alpern RJ. Chronic metabolic acidosis increases NHE3 protein abundance in rat kidney. American Journal Of Physiology 1996, 271: f917-f925. PMID: 8898023, DOI: 10.1152/ajprenal.1996.271.4.f917.Peer-Reviewed Original ResearchConceptsNHE3 protein abundanceChronic metabolic acidosisNHE3 mRNA abundanceMetabolic acidosisThick ascending limbProximal tubulesAscending limbNa/H antiporter activityAcidosis-induced increaseMRNA abundanceNa/H antiporterNHE3 abundanceProtein abundanceAcidosisRat kidneyWestern blotS2 segmentsH antiporterRatsNHE3 isoformPresent studyLimbTubules
1995
Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat
LaPointe M, Ye M, Moe O, Alpern R, Batlle D. Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat. Kidney International 1995, 47: 78-87. PMID: 7731174, DOI: 10.1038/ki.1995.9.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsCultured aortic vascular smooth muscle cellsWistar/Kyoto ratsAortic vascular smooth muscle cellsWKY ratsHypertensive ratsNominal absenceCultured vascular smooth muscleVascular smooth muscleSmooth muscle cellsCell acidificationRate of intracellularNormotensive controlsKyoto ratsNHE-1 isoformWKY cellsSmooth muscleSHRHigh functional activityRatsMuscle cellsThymidine incorporationPresence of HCO3Exchange activityHCO3-/CO2
1991
Dietary NaCl modulates Na(+)-H+ antiporter activity in renal cortical apical membrane vesicles
Moe OW, Tejedor A, Levi M, Seldin DW, Preisig PA, Alpern RJ. Dietary NaCl modulates Na(+)-H+ antiporter activity in renal cortical apical membrane vesicles. American Journal Of Physiology 1991, 260: f130-f137. PMID: 1847012, DOI: 10.1152/ajprenal.1991.260.1.f130.Peer-Reviewed Original ResearchConceptsDietary NaClNaCl reabsorptionSystemic acid-base disturbancesExtracellular fluid volume depletionNaHCO3 reabsorptionExtracellular fluid volume expansionFluid volume depletionHigh-salt dietChronic metabolic alkalosisFluid volume expansionAcridine orange fluorescence methodExtracellular fluid volumeAcid-base disturbancesAntiporter activityDietary protocolChronic alterationsVolume depletionMetabolic alkalosisChronic changesHS dietApical membrane vesiclesLS dietFluid volumeRatsReabsorption
1989
Basolateral membrane H-OH-HCO3 transport in the proximal tubule
Preisig P, Alpern R. Basolateral membrane H-OH-HCO3 transport in the proximal tubule. American Journal Of Physiology 1989, 256: f751-f765. PMID: 2541627, DOI: 10.1152/ajprenal.1989.256.5.f751.Peer-Reviewed Original ResearchConceptsProximal tubulesS3 proximal tubuleChronic respiratory acidosisChronic metabolic acidosisRabbit proximal tubuleChronic hyperfiltrationMetabolic acidosisNa-H antiporterRespiratory acidosisCl-HCO3 exchangerCl absorptionGreater transport capacityAlkalosisAcidosisTubulesTransporter activityMembrane mechanismsBasolateral membraneCotransporterHyperfiltrationRatsEpithelium
1985
Mechanism of basolateral membrane H+/OH-/HCO-3 transport in the rat proximal convoluted tubule. A sodium-coupled electrogenic process.
Alpern RJ. Mechanism of basolateral membrane H+/OH-/HCO-3 transport in the rat proximal convoluted tubule. A sodium-coupled electrogenic process. The Journal Of General Physiology 1985, 86: 613-636. PMID: 2999293, PMCID: PMC2228817, DOI: 10.1085/jgp.86.5.613.Peer-Reviewed Original ResearchConceptsUnits/minPeritubular pHHigh extracellular potassium concentrationCell pHSodium concentrationPotassium concentrationExtracellular potassium concentrationBasolateral membranePeritubular potassium concentrationCell depolarizationMeq/Sodium removalCell acidificationTubulesPeritubular fluidTotal absenceSitCellsRatsMinSodium
1983
Effects of extracellular fluid volume and plasma bicarbonate concentration on proximal acidification in the rat.
Alpern RJ, Cogan MG, Rector FC. Effects of extracellular fluid volume and plasma bicarbonate concentration on proximal acidification in the rat. Journal Of Clinical Investigation 1983, 71: 736-746. PMID: 6826733, PMCID: PMC436924, DOI: 10.1172/jci110821.Peer-Reviewed Original ResearchConceptsNet bicarbonate absorptionExtracellular fluid volumeVolume-expanded ratsLuminal bicarbonate concentrationPlasma bicarbonate concentrationBicarbonate absorptionVolume statusFluid volumeBicarbonate concentrationProton secretionExtracellular fluid volume statusBicarbonate permeabilityFluid volume statusPeritubular bicarbonate concentrationsProximal acidificationHydropenic ratsRatsAcidotic ratsSecretionAbove inhibitionLuminal compositionAlkalosisVolume expansionTubulesMM bicarbonate
1982
Effect of luminal bicarbonate concentration on proximal acidification in the rat
Alpern RJ, Cogan MG, Rector FC. Effect of luminal bicarbonate concentration on proximal acidification in the rat. American Journal Of Physiology 1982, 243: f53-f59. PMID: 7091367, DOI: 10.1152/ajprenal.1982.243.1.f53.Peer-Reviewed Original ResearchConceptsLuminal bicarbonate concentrationBicarbonate absorptionBicarbonate concentrationNet bicarbonate absorptionProximal convoluted tubulesProximal tubular acidificationProton secretionBicarbonate leakProximal acidificationTubular acidificationConvoluted tubulesBicarbonate permeabilitySecretionRats