2020
Examining the role of muscarinic M5 receptors in VTA cholinergic modulation of depressive-like and anxiety-related behaviors in rats
Nunes EJ, Rupprecht LE, Foster DJ, Lindsley CW, Conn PJ, Addy NA. Examining the role of muscarinic M5 receptors in VTA cholinergic modulation of depressive-like and anxiety-related behaviors in rats. Neuropharmacology 2020, 171: 108089. PMID: 32268153, PMCID: PMC7313677, DOI: 10.1016/j.neuropharm.2020.108089.Peer-Reviewed Original ResearchConceptsSucrose preference testVentral tegmental areaMuscarinic M5 receptorsCholinergic toneM5 receptorsBehavioral effectsFemale ratsDepressive-like behavioral effectsIntra-VTA infusionAnxiogenic-like effectsAcetylcholinesterase inhibitor physostigmineAnxiety-related behaviorVTA administrationCholinergic modulationCholinergic receptorsMuscarinic receptorsInhibitor physostigmineSwim testTegmental areaMale ratsMood disordersVTA infusionsFST behaviorRatsPhysostigmine
2019
Cholinergic Receptor Blockade in the VTA Attenuates Cue-Induced Cocaine-Seeking and Reverses the Anxiogenic Effects of Forced Abstinence
Nunes EJ, Bitner L, Hughley SM, Small KM, Walton SN, Rupprecht LE, Addy NA. Cholinergic Receptor Blockade in the VTA Attenuates Cue-Induced Cocaine-Seeking and Reverses the Anxiogenic Effects of Forced Abstinence. Neuroscience 2019, 413: 252-263. PMID: 31271832, PMCID: PMC6661179, DOI: 10.1016/j.neuroscience.2019.06.028.Peer-Reviewed Original ResearchConceptsCue-induced cocaineAnxiety-related behaviorDrug relapseOpen arm timeCholinergic receptor blockadeCocaine abstinenceVentral tegmental areaSubstance abuseSelf-administer intravenous cocaineMuscarinic receptor antagonist scopolaminePeriod of abstinenceAnxiogenic effectsReceptor blockadeCholinergic mechanismsCocaine-naïve ratsNicotinic acetylcholine receptor antagonist mecamylamineMale Sprague-Dawley ratsDrug abstinenceSprague-Dawley ratsDose-dependent increaseCocaine-naive ratsAntagonist scopolamineEPM behaviorSaline ratsAbstinence