A Glutamine Switch Mechanism for Nucleotide Selectivity by Phosphodiesterases
Zhang KY, Card GL, Suzuki Y, Artis DR, Fong D, Gillette S, Hsieh D, Neiman J, West BL, Zhang C, Milburn MV, Kim SH, Schlessinger J, Bollag G. A Glutamine Switch Mechanism for Nucleotide Selectivity by Phosphodiesterases. Molecular Cell 2004, 15: 279-286. PMID: 15260978, DOI: 10.1016/j.molcel.2004.07.005.Peer-Reviewed Original ResearchMeSH Keywords3',5'-Cyclic-AMP Phosphodiesterases3',5'-Cyclic-GMP PhosphodiesterasesCatalytic DomainCrystallography, X-RayCyclic AMPCyclic GMPCyclic Nucleotide Phosphodiesterases, Type 3Cyclic Nucleotide Phosphodiesterases, Type 4Cyclic Nucleotide Phosphodiesterases, Type 5GlutamineHumansModels, MolecularProtein ConformationConceptsNucleotide selectivityKey specificity determinantKey histidine residuesFamily of enzymesHigh-resolution co-crystal structuresCo-crystal structureNew PDE inhibitorsGlutamine switchInvariant glutamineSpecificity determinantsTypes of phosphodiesterasesGlutamine functionsGlutamine residuesHistidine residuesSwitch mechanismStructural understandingPhosphodiesterasesCyclic nucleotidesResiduesCritical rolePurine moietyCGMPCAMPPDE inhibitorsNucleotides