2001
G-Protein Signaling Through Tubby Proteins
Santagata S, Boggon T, Baird C, Gomez C, Zhao J, Shan W, Myszka D, Shapiro L. G-Protein Signaling Through Tubby Proteins. Science 2001, 292: 2041-2050. PMID: 11375483, DOI: 10.1126/science.1061233.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAdaptor Proteins, Signal TransducingAmino Acid SequenceAnimalsCell MembraneCell NucleusCells, CulturedCrystallography, X-RayGene Expression RegulationGTP-Binding Protein alpha Subunits, Gq-G11Heterotrimeric GTP-Binding ProteinsHumansIntercellular Signaling Peptides and ProteinsIntracellular Signaling Peptides and ProteinsIsoenzymesMembrane LipidsMiceModels, BiologicalMolecular Sequence DataNuclear Localization SignalsObesityPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol PhosphatesPhospholipase C betaPhosphorylationProtein Structure, TertiaryProteinsReceptor, Serotonin, 5-HT2CReceptors, MuscarinicReceptors, SerotoninRecombinant Fusion ProteinsSignal TransductionTranscription FactorsType C PhospholipasesConceptsTubby-like protein 3G protein signalingTubby proteinTubby domainTranscription regulatorsPlasma membranePhospholipase C-betaReceptor-mediated activationHeterotrimeric GTPSignal transductionGene expressionMolecular mechanismsTubbyC betaCarboxyl terminalCell nucleiMaturity-onset obesityProteinX-ray crystallographyProtein 3SignalingRegulatorMembranePhosphatidylinositolTransduction
1999
Implication of Tubby Proteins as Transcription Factors by Structure-Based Functional Analysis
Boggon T, Shan W, Santagata S, Myers S, Shapiro L. Implication of Tubby Proteins as Transcription Factors by Structure-Based Functional Analysis. Science 1999, 286: 2119-2125. PMID: 10591637, DOI: 10.1126/science.286.5447.2119.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAlternative SplicingAmino Acid SequenceAnimalsCell LineCell NucleusCrystallography, X-RayDNAEye ProteinsHumansIntercellular Signaling Peptides and ProteinsIntracellular Signaling Peptides and ProteinsModels, MolecularMolecular Sequence DataProtein ConformationProtein Structure, SecondaryProtein Structure, TertiaryProteinsRecombinant ProteinsSequence AlignmentTranscription FactorsTranscriptional ActivationConceptsTubby-like proteinsTubby proteinTranscription factorsBipartite transcription factorDisease phenotypeMulticellular organismsProtein familyBiochemical functionsBiological functionsFunctional analysisStructural cluesCore domainUnique familyProteinGenetic mutationsTubbyPhenotypeRetinal degenerationFamilyMammalsOrganismsVital roleCrystal structureMutationsBroad rangeBound‐solvent structures for microgravity‐, ground control‐, gel‐ and microbatch‐grown hen egg‐white lysozyme crystals at 1.8 Å resolution
Dong J, Boggon T, Chayen N, Raftery J, Bi R, Helliwell J. Bound‐solvent structures for microgravity‐, ground control‐, gel‐ and microbatch‐grown hen egg‐white lysozyme crystals at 1.8 Å resolution. Acta Crystallographica Section D, Structural Biology 1999, 55: 745-752. PMID: 10089304, DOI: 10.1107/s0907444998016047.Peer-Reviewed Original ResearchConceptsHen egg white lysozymeX-ray dataAdvanced Protein Crystallization FacilityDifferent crystallization methodsLysozyme crystalsCrystal growth environmentProtein structureHen egg-white lysozyme crystalsEgg white lysozymeCrystallization methodProtein crystalsGelÅ resolutionCrystalsMicrogravity Spacelab (LMS) missionCrystallizationCrystallization FacilityAgarose gelStructureDiffusion methodPhase boundaryPhysics statesOilNumber of methodsLysozyme
1996
The Crystal Structure Analysis of d(CGCGAASSCGCG)2, a Synthetic DNA Dodecamer Duplex Containing Four 4′-Thio-2′-Deoxythymidine Nucleotides
Boggon T, Hancox E, McAuley-Hecht K, Connolly B, Hunter W, Brown T, Walker R, Leonard G. The Crystal Structure Analysis of d(CGCGAASSCGCG)2, a Synthetic DNA Dodecamer Duplex Containing Four 4′-Thio-2′-Deoxythymidine Nucleotides. Nucleic Acids Research 1996, 24: 951-961. PMID: 8600465, PMCID: PMC145717, DOI: 10.1093/nar/24.5.951.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCrystallizationCrystallography, X-RayDNAMolecular Sequence DataMolecular StructureThionucleotidesThymidineConceptsDodecamer duplexCrystal structure analysisC1'-exo conformationC3'-exo conformationFine structural parametersCrystal structure refinementSugar-phosphate backboneSynthetic dodecamerWater moleculesDNA dodecamer duplexAnalogous sugarsStructure analysisStructure refinementNative dodecamerConformationDodecamerStructural parametersDuplexDeoxythymidine nucleotidesStructureThiosugarsBackboneMoleculesNucleosidesSynthetic