Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins
Page RC, Moore JD, Nguyen HB, Sharma M, Chase R, Gao FP, Mobley CK, Sanders CR, Ma L, Sönnichsen FD, Lee S, Howell SC, Opella SJ, Cross TA. Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins. Journal Of Structural And Functional Genomics 2006, 7: 51-64. PMID: 16850177, DOI: 10.1007/s10969-006-9009-9.Peer-Reviewed Original ResearchConceptsHelical integral membrane proteinsIntegral membrane proteinsNuclear magnetic resonance spectroscopyMembrane proteinsStructural characterizationSolution nuclear magnetic resonance spectroscopyChemical shift correlation spectraRobust sample preparation methodSample preparationChemical shift correlationSolution NMR spectraSolution NMR spectroscopyShift correlation spectraSample preparation methodSample preparation protocolProper sample preparationEfficient purification protocolMagnetic resonance spectroscopyNMR spectroscopyShift correlationNMR spectraPreparation methodTransmembrane helicesResonance spectroscopyCorrelation spectra