2006
Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins
Page RC, Moore JD, Nguyen HB, Sharma M, Chase R, Gao FP, Mobley CK, Sanders CR, Ma L, Sönnichsen FD, Lee S, Howell SC, Opella SJ, Cross TA. Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins. Journal Of Structural And Functional Genomics 2006, 7: 51-64. PMID: 16850177, DOI: 10.1007/s10969-006-9009-9.Peer-Reviewed Original ResearchConceptsHelical integral membrane proteinsIntegral membrane proteinsNuclear magnetic resonance spectroscopyMembrane proteinsStructural characterizationSolution nuclear magnetic resonance spectroscopyChemical shift correlation spectraRobust sample preparation methodSample preparationChemical shift correlationSolution NMR spectraSolution NMR spectroscopyShift correlation spectraSample preparation methodSample preparation protocolProper sample preparationEfficient purification protocolMagnetic resonance spectroscopyNMR spectroscopyShift correlationNMR spectraPreparation methodTransmembrane helicesResonance spectroscopyCorrelation spectra
1999
Solution structure of neuromedin B by 1H nuclear magnetic resonance spectroscopy
Lee S, Kim Y. Solution structure of neuromedin B by 1H nuclear magnetic resonance spectroscopy. FEBS Letters 1999, 460: 263-269. PMID: 10544247, DOI: 10.1016/s0014-5793(99)01346-0.Peer-Reviewed Original ResearchConceptsNuclear magnetic resonance spectroscopySDS micellesMagnetic resonance spectroscopyTwo-dimensional nuclear magnetic resonance spectroscopyResonance spectroscopyAromatic ring protonsSolution structureMembrane-mimicking environmentHydrophobic acyl chainsStructure-activity relationshipsMethylene protonsLongitudinal relaxation dataNOESY experimentsHelical conformationConformational featuresRing protonsMicellesMolecular mechanismsSpectroscopyAcyl chainsExtrinsic interactionsRelaxation dataEfficient drugsResiduesProtonsStudies of the Chemical Structure of Gangliosides in Deer Antler, Cervus nippon
Jhon G, Park S, Han S, Lee S, Kim Y, Chang Y. Studies of the Chemical Structure of Gangliosides in Deer Antler, Cervus nippon. Chemical And Pharmaceutical Bulletin 1999, 47: 123. PMID: 9987834, DOI: 10.1248/cpb.47.123.Peer-Reviewed Original ResearchConceptsHigh-field proton nuclear magnetic resonance spectroscopyFast atom bombardment mass spectrometry studiesProton nuclear magnetic resonance spectroscopyNuclear magnetic resonance spectroscopyGas chromatography/mass spectrometryChromatography/mass spectrometrySilica gel column chromatographyGel column chromatographyDirect compositional analysisDEAE-Sephadex AMagnetic resonance spectroscopyN-acetyl GM3Amperometric detectionChemical structurePrior derivatizationSpectrometry studiesStructural determinationTrifluoroacetic acidMass spectrometryResonance spectroscopyColumn chromatographyStandard monosaccharidesBiological activityStructure of gangliosidesCeramide moiety