1996
KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells
Cui Z, Hidaka H, Dannies P. KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells. Biochimica Et Biophysica Acta 1996, 1310: 343-347. PMID: 8599613, DOI: 10.1016/0167-4889(95)00170-0.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAnimalsCalciumCalcium-Calmodulin-Dependent Protein KinasesCells, CulturedEnzyme InhibitorsFemaleIsoquinolinesKineticsPiperazinesPituitary Gland, AnteriorPotassiumPotassium ChlorideProlactinRatsRats, Sprague-DawleyThyrotropin-Releasing HormoneTime FactorsConceptsAnterior pituitary cellsProlactin secretionCalcium/calmodulin-dependent protein kinase IIPituitary cellsCalmodulin-dependent protein kinase IIKN-04KN-62Rat anterior pituitary cellsVoltage-dependent calcium channelsProtein kinase IICalmodulin-dependent protein kinase II inhibitorKCl-stimulated increasesIntracellular calcium increaseCalcium/calmodulin-dependent protein kinase II inhibitorKinase II inhibitorCalcium channelsCalcium increaseIntracellular Ca2Kinase IISecretionInhibits activationII inhibitorsIC50InhibitorsCells
1991
Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretion
Pachter J, Law G, Dannies P. Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretion. European Journal Of Pharmacology 1991, 195: 373-379. PMID: 1714395, DOI: 10.1016/0014-2999(91)90478-9.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCalciumCalcium Channel AgonistsCalcium Channel BlockersCalcium RadioisotopesDihydropyridinesFemaleIn Vitro TechniquesInositol PhosphatesNimodipinePerfusionProlactinPyridinesRatsRats, Inbred StrainsReceptors, PurinergicThyrotropin-Releasing HormoneConceptsBay K 8644Basal prolactin secretionProlactin secretionChannel activator Bay K 8644Potentiation of TRHChannel antagonist nimodipineAnterior pituitary cellsAnterior pituitary tumor cellsEnhancement of basalAccumulation of inositolSDZ 202Antagonist nimodipinePituitary tumor cellsInositol polyphosphate accumulationCGP 28392Dihydropyridine Ca2Hormone secretionChannel agonistDihydropyridine agonistPituitary cellsTRHTumor cellsSecretionGH4C1 cellsPrimary cultures
1990
Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells
Law G, Pachter J, Thastrup O, Hanley M, Dannies P. Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells. Biochemical Journal 1990, 267: 359-364. PMID: 1692207, PMCID: PMC1131296, DOI: 10.1042/bj2670359.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCaffeineCalciumCell LineInositol PhosphatesKineticsNimodipinePituitary NeoplasmsPlants, MedicinalProlactinTerpenesThapsigarginThyrotropin-Releasing HormoneTumor Cells, CulturedConceptsThyrotropin-releasing hormoneGH4C1 pituitary cellsPituitary cellsIntracellular storesGH4C1 cellsAbility of TRHBay K 8644Cytosolic free Ca2Action of thapsigarginInsP3-sensitive storesSustained elevationChannel blockersReceptor levelsTRH stimulationExtracellular Ca2Intracellular Ca2Transient increaseFree Ca2ThapsigarginSubsequent spikesHormoneCell typesCa2CellsEfflux
1989
Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of Prolactin
Law G, Pachter J, Dannies P. Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of Prolactin. Endocrinology 1989, 3: 539-546. PMID: 2501668, DOI: 10.1210/mend-3-3-539.Peer-Reviewed Original ResearchConceptsPulses of TRHPRL releasePRL secretionCytosolic free calcium concentrationPost-receptor stepsRat anterior pituitary glandThyrotropin-Releasing HormoneRelease of prolactinIndicator indo-1Anterior pituitary glandFree calcium concentrationRelationship of changesContinuous administrationSustained elevationNM TRHPituitary glandIndo-1TRHFluorescent Ca2Calcium concentrationRefractory stateTransient spikeSecretionMinReleaseAbility of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependent
Law G, Pachter J, Dannies P. Ability of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependent. Biochemical And Biophysical Research Communications 1989, 158: 811-816. PMID: 2537637, DOI: 10.1016/0006-291x(89)92794-0.Peer-Reviewed Original ResearchConceptsProlactin releaseAnterior pituitary cellsRelease of prolactinSustained elevated levelsCytosolic free Ca2Amount of prolactinSerial declinePeak Ca2Prolactin secretionPerifusion systemPituitary cellsIndo-1Fluorescent Ca2Repetitive Ca2Transient increaseFree Ca2Elevated levelsProlactinMM KClCa2CellsCoincident increaseMinutesReleaseHigh frequency
1988
Dopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary Cells
Law G, Pachter J, Dannies P. Dopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary Cells. Endocrinology 1988, 2: 966-972. PMID: 3141796, DOI: 10.1210/mend-2-10-966.Peer-Reviewed Original ResearchConceptsRat anterior pituitary cellsAnterior pituitary cellsPRL secretionPituitary cellsIntracellular Ca2Intracellular storesLow Ca2MM Ca2Inhibition of releaseDopaminergic reductionSource of Ca2PRL releaseIntracellular calciumNM TRHPerifusion systemExtracellular Ca2MicroM dopamineTRHInternal Ca2DopamineEnriched populationMM KClSecretionInositol phosphatesCa2
1985
Stimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*
DELBEKE D, DANNIES P. Stimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*. Endocrinology 1985, 117: 439-446. PMID: 2990850, DOI: 10.1210/endo-117-2-439.Peer-Reviewed Original ResearchConceptsPRL releaseDopaminergic inhibitionAbsence of dopamineMessenger systemsRat anterior pituitary cellsPattern of releaseAnterior pituitary cellsCAMP messenger systemMin of preincubationBasal releaseProlactin releaseNM TRHPituitary cellsIntracellular Ca2Sustained phasePhorbol ester 12Cytosolic Ca2Intracellular cAMPTRHAdenylate cyclaseCAMP levelsDopamineSustained releaseLevel of releaseC-kinase activity
1984
Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin
Delbeke D, Kojima I, Dannies P, Rasmussen H. Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin. Biochemical And Biophysical Research Communications 1984, 123: 735-741. PMID: 6091639, DOI: 10.1016/0006-291x(84)90291-2.Peer-Reviewed Original ResearchDifference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*
DELBEKE D, SCAMMELI J, DANNIES P. Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*. Endocrinology 1984, 114: 1433-1440. PMID: 6323146, DOI: 10.1210/endo-114-4-1433.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPRL releaseMale rat pituitary glandPrimary culturesNormal cellsNormal pituitary cellsPresence of D600Release of prolactinRat pituitary glandAddition of forskolinIntracellular cAMP concentrationPituitary tumorsFischer ratsPituitary cellsPituitary glandChannel inhibitorsTumor cellsTypes of cellsAdenylate cyclaseD600ForskolinCAMP concentrationCalcium requirementForskolin stimulationCells
1983
Veratridine and Ouabain Stimulate Calcium-Dependent Prolactin Release*
SCAMMELL J, DANNIES P. Veratridine and Ouabain Stimulate Calcium-Dependent Prolactin Release*. Endocrinology 1983, 113: 1228-1235. PMID: 6617570, DOI: 10.1210/endo-113-4-1228.Peer-Reviewed Original ResearchConceptsPRL releaseCalcium channel blocker D600Rat anterior pituitary cellsTetrodotoxin-insensitive mechanismCalcium channel blockersChannel blocker tetrodotoxinDopaminergic agonist bromocriptineEffect of veratridineAnterior pituitary cellsAction of veratridineChannel blocker D600Blocker tetrodotoxinAgonist bromocriptineProlactin releaseChannel blockersVeratridine effectPituitary cellsChannel activatorVeratridineGH4C1 cellsPrimary culturesOuabainNormal cellsStimulationPreincubation
1982
Prolactin: Multiple intracellular processing routes plus several potential mechanisms for regulation
Dannies P. Prolactin: Multiple intracellular processing routes plus several potential mechanisms for regulation. Biochemical Pharmacology 1982, 31: 2845-2849. PMID: 6291547, DOI: 10.1016/0006-2952(82)90253-2.Peer-Reviewed Original Research
1980
Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells.
Tam S, Dannies P. Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells. Journal Of Biological Chemistry 1980, 255: 6595-6599. PMID: 6771274, DOI: 10.1016/s0021-9258(18)43611-3.Peer-Reviewed Original Research
1971
Structural Properties of Bovine Brain S-100 Protein
Dannies P, Levine L. Structural Properties of Bovine Brain S-100 Protein. Journal Of Biological Chemistry 1971, 246: 6276-6283. PMID: 5001787, DOI: 10.1016/s0021-9258(18)61785-5.Peer-Reviewed Original ResearchAlkylationAmino AcidsAmmonium SulfateAnimalsBenzoatesBrainCalciumCattleChemical PrecipitationChromatography, GelChromatography, Ion ExchangeDisulfidesImmune SeraImmunodiffusionIodoacetatesKineticsMacromolecular SubstancesMagnesiumMaleatesMercaptoethanolMolecular WeightNerve Tissue ProteinsNitro CompoundsProtein ConformationStrontiumSulfhydryl CompoundsTrypsinTryptophanUltracentrifugationUrea