2000
Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH*
Lee M, Wajnrajch M, Kim S, Plotnick L, Wang J, Gertner J, Leibel R, Dannies P. Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH*. Endocrinology 2000, 141: 883-890. DOI: 10.1210/en.141.3.883.Peer-Reviewed Original ResearchWild-type GHGH deficiency type IIGrowth hormoneWild-type human GHSecretory pathway functionGH deficiencyAutosomal dominant formHuman GHDel32Neuroendocrine cell lineShort statureHuman PRLNormal alleleTransient transfectionCell linesPosttranslational effectWild-typeTransfected COSDecreased synthesisPRLIntracellular stabilityPathway functionCHO cellsType IISuppressed accumulation
1997
Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+.
Sun Z, Lee M, Rhee H, Arrandale J, Dannies P. Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+. Endocrinology 1997, 11: 1544-51. PMID: 9280069, DOI: 10.1210/mend.11.10.0002.Peer-Reviewed Original Research
1995
Biological activity and immunological reactivity of human prolactin mutants
Rhee H, Sun Z, Kim S, Goffin V, Martial J, Dannies P. Biological activity and immunological reactivity of human prolactin mutants. Endocrinology 1995, 136: 4990-4995. DOI: 10.1210/en.136.11.4990.Peer-Reviewed Original ResearchLack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage
Mastro R, Dannies P. Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage. Endocrinology 1995, 136: 69-74. PMID: 7828559, DOI: 10.1210/endo.136.1.7828559.Peer-Reviewed Original ResearchConceptsEpidermal growth factorNormal lactotrophsGH4C1 cellsGrowth factorForms of PRLFemale rat pituitary glandsNM epidermal growth factorRat pituitary glandDense core granulesRat pituitary tumor cellsPRL storagePituitary tumor cellsNM estradiolTotal PRLPituitary glandPRLTumor cellsCore granulesInsulinNM insulinLack of correlationLactotrophs
1994
Inhibition of rat prolactin (PRL) storage by coexpression of human PRL.
Arrandale J, Dannies P. Inhibition of rat prolactin (PRL) storage by coexpression of human PRL. Endocrinology 1994, 8: 1083-1090. PMID: 7997234, DOI: 10.1210/mend.8.8.7997234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAsparagineBiological TransportCell CompartmentationCytoplasmic GranulesEpidermal Growth FactorEstradiolGene ExpressionHumansInsulinModels, MolecularPituitary NeoplasmsProlactinProtein ConformationRatsRecombinant Fusion ProteinsSerineSpecies SpecificityTransfectionTumor Cells, CulturedConceptsHuman PRLRat PRLGH4C1 cellsReceptor-mediated mechanismNM epidermal growth factorSecretory granulesNM estradiolDense-core secretory granulesEpidermal growth factorHormone treatmentPRL storageProlactin storagePRLGrowth factorNM insulinMore ratsUntransfected cellsRatsRegulated pathwayControl culturesCellsInhibitionMarked specificity
1990
Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated.
Reaves B, Van Itallie C, Moore H, Dannies P. Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated. Endocrinology 1990, 4: 1017-26. PMID: 2284004, DOI: 10.1210/mend-4-7-1017.Peer-Reviewed Original ResearchConceptsGH4C1 cellsEpidermal growth factorPRL synthesisGrowth factorPreferential increaseCombination of estradiolRegulated pathwayRat pituitary tumor cellsIntracellular PRLPituitary tumor cellsBasal releasePRL releaseIntracellular proinsulinSame time courseHormone treatmentPRL storageTumor cellsProinsulin immunoreactivityMRNA levelsPRLInsulinSame peak heightHormoneProinsulinTreatment
1986
Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*
SCAMMELL J, BURRAGE T, DANNIES P. Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*. Endocrinology 1986, 119: 1543-1548. PMID: 3530721, DOI: 10.1210/endo-119-4-1543.Peer-Reviewed Original ResearchConceptsGH4C1 cellsIntracellular PRLSecretory granulesCombination of estradiolNumber of granulesPituitary tumor cell linePRL accumulationHormone regimenTumor cell strainsEpidermal growth factorHormone treatmentTumor cell linesPRLGrowth factorControl levelsOccasional granulesCell linesHormonal inductionGHCell strainsCellsGranule numberTreatmentCellular contentStorage granules
1985
Cysteamine Causes Reduction of Prolactin Monomers Followed by Aggregation in the Rat Pituitary Gland*
SCAMMELL J, BURRAGE T, EISENFELD A, DANNIES P. Cysteamine Causes Reduction of Prolactin Monomers Followed by Aggregation in the Rat Pituitary Gland*. Endocrinology 1985, 116: 2347-2354. PMID: 3996317, DOI: 10.1210/endo-116-6-2347.Peer-Reviewed Original Research
1983
3COMPARISON OF THEEFFECTS OF HISTAMINE H2-RECEPTOR ANTAGONISTS ON PROLACTIN SECRETION IN THE RAT.
NETTI C, GUIDOBONO F, OLGIATI V, SIBILIA V, PECILE A, Dannies P. 3COMPARISON OF THEEFFECTS OF HISTAMINE H2-RECEPTOR ANTAGONISTS ON PROLACTIN SECRETION IN THE RAT. Endocrinology 1983, 113: 412-414. PMID: 6305638, DOI: 10.1210/endo-113-1-412.Peer-Reviewed Original ResearchConceptsPRL releaseHistamine H2-receptor antagonistsSignificant PRL releaseH2-receptor antagonistsCentral neural controlPRL-releasing activityHistamine H2 receptorsPRL secretionBlocking actionProlactin secretionH2 receptorsSingle bolusBlood samplesHigh doseBlocking potencyPrompt increaseBrain ventriclesNeural controlDrugsCimetidineRanitidineRatsStimulating effectPRLSecretion
1981
Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*
KIINO D, DANNIES P. Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*. Endocrinology 1981, 109: 1264-1269. PMID: 7026222, DOI: 10.1210/endo-109-4-1264.Peer-Reviewed Original ResearchConceptsIntracellular PRLEffect of insulinDays of treatmentHalf-maximal doseRat pituitary tumor cellsPituitary tumor cellsIntracellular prolactin contentM estradiolEstradiolCombined treatmentPRLInsulinPRL synthesisTumor cellsGH4C1 cellsM insulinControl levelsProlactin contentIntracellular hormoneM. TreatmentTotal proteinTreatmentDoseIntracellular transit timeSmall increase
1980
Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*
DANNIES P, TASHJIAN A. Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*. Endocrinology 1980, 106: 1532-1536. PMID: 6244936, DOI: 10.1210/endo-106-5-1532.Peer-Reviewed Original ResearchConceptsGH synthesisCholera toxinIntracellular cAMPWeeks of treatmentThyrotropin-releasing hormoneRelease of PRLHormone synthesisRat pituitary cellsIntracellular PRLGrowth hormone synthesisPRL releaseSimilar lag periodPituitary cellsGH cellsControl valuesPRL synthesisTRHPRLToxinClonal strainsLag periodReleaseCellsCAMPDecrease
1979
Antipsychotic Drugs Inhibit Prolactin Release from Rat Anterior Pituitary Cells in Culture by a Mechanism Not Involving the Dopamine Receptor*
WEST B, DANNIES P. Antipsychotic Drugs Inhibit Prolactin Release from Rat Anterior Pituitary Cells in Culture by a Mechanism Not Involving the Dopamine Receptor*. Endocrinology 1979, 104: 877-880. PMID: 436760, DOI: 10.1210/endo-104-4-877.Peer-Reviewed Original ResearchConceptsRat anterior pituitary cellsAnterior pituitary cellsGH releasePRL releaseControl valuesAntipsychotic drugsDopamine antagonistsDopamine receptorsPituitary cellsInhibition of PRLMicroM pimozideDopamine agonistsHormone releaseD-butaclamolInhibited secretionPRLBromocriptineAntagonistButaclamolInhibitionReceptorsDrugsReleaseCellsHaloperidol