2014
MicroRNA mimicry blocks pulmonary fibrosis
Montgomery RL, Yu G, Latimer PA, Stack C, Robinson K, Dalby CM, Kaminski N, van Rooij E. MicroRNA mimicry blocks pulmonary fibrosis. EMBO Molecular Medicine 2014, 6: 1347-1356. PMID: 25239947, PMCID: PMC4287936, DOI: 10.15252/emmm.201303604.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBleomycinBlotting, NorthernCell LineCell Line, TumorGene Expression ProfilingGene Expression RegulationHumansMaleMiceMice, Inbred C57BLMicroRNAsMolecular MimicryNIH 3T3 CellsPulmonary FibrosisReverse Transcriptase Polymerase Chain ReactionAging Mesenchymal Stem Cells Fail to Protect Because of Impaired Migration and Antiinflammatory Response
Bustos ML, Huleihel L, Kapetanaki MG, Lino-Cardenas CL, Mroz L, Ellis BM, McVerry BJ, Richards TJ, Kaminski N, Cerdenes N, Mora AL, Rojas M. Aging Mesenchymal Stem Cells Fail to Protect Because of Impaired Migration and Antiinflammatory Response. American Journal Of Respiratory And Critical Care Medicine 2014, 189: 787-798. PMID: 24559482, PMCID: PMC4061541, DOI: 10.1164/rccm.201306-1043oc.Peer-Reviewed Original ResearchMeSH KeywordsAcute Lung InjuryAgingAnimalsBiomarkersBone Marrow CellsCell MovementChemokinesCytokinesDown-RegulationFemaleMesenchymal Stem CellsMiceMice, Inbred C57BLOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionWound HealingConceptsBone marrow-derived MSCsMesenchymal stem cellsChemokine receptorsAcute lung injuryAcute lung diseaseSite of injuryAge-dependent decreaseBone marrow-derived mesenchymal stem cellsMarrow-derived mesenchymal stem cellsInflammatory response genesSuch age-related changesAge-related phenomenonStem cellsAge-related changesAdoptive transferLung injuryEndotoxemic miceLung diseaseAntiinflammatory responseFunctional impairmentMore inflammationProtective effectOld miceParacrine mechanismsAlveolar epithelium
2013
Peripheral Blood Mononuclear Cell Gene Expression Profiles Predict Poor Outcome in Idiopathic Pulmonary Fibrosis
Herazo-Maya JD, Noth I, Duncan SR, Kim S, Ma SF, Tseng GC, Feingold E, Juan-Guardela BM, Richards TJ, Lussier Y, Huang Y, Vij R, Lindell KO, Xue J, Gibson KF, Shapiro SD, Garcia JG, Kaminski N. Peripheral Blood Mononuclear Cell Gene Expression Profiles Predict Poor Outcome in Idiopathic Pulmonary Fibrosis. Science Translational Medicine 2013, 5: 205ra136. PMID: 24089408, PMCID: PMC4175518, DOI: 10.1126/scitranslmed.3005964.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCD28 AntigensCD4 AntigensCluster AnalysisCohort StudiesGene Expression ProfilingHumansIdiopathic Pulmonary FibrosisLeukocytes, MononuclearOligonucleotide Array Sequence AnalysisReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSignal TransductionTreatment OutcomeConceptsTransplant-free survivalIdiopathic pulmonary fibrosisPeripheral blood mononuclear cell gene expression profilesReplication cohortCell gene expression profilesPoor outcomePulmonary fibrosisQuantitative reverse transcription polymerase chain reactionReverse transcription-polymerase chain reactionProportional hazards modelTranscription-polymerase chain reactionGene expression profilesPotential cellular sourcesT cell activationIPF patientsLung transplantationMicroarray cohortPatient ageOutcome biomarkerPatient groupVital capacityPolymerase chain reactionT cellsDiscovery cohortITK expression
2011
Mouse Conjunctival Forniceal Gene Expression during Postnatal Development and Its Regulation by Krüppel-like Factor 4
Gupta D, Harvey SA, Kaminski N, Swamynathan SK. Mouse Conjunctival Forniceal Gene Expression during Postnatal Development and Its Regulation by Krüppel-like Factor 4. Investigative Ophthalmology & Visual Science 2011, 52: 4951-4962. PMID: 21398290, PMCID: PMC3176043, DOI: 10.1167/iovs.10-7068.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsConjunctivaEpithelial CellsFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGenotypeGoblet CellsIn Situ HybridizationKruppel-Like Factor 4Kruppel-Like Transcription FactorsMaleMiceOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionTranscription FactorsConceptsKLF4 target genesGene expressionTarget genesEts transcription factor family memberCell developmentTranscription factor family membersGene regulatory networksGoblet cell developmentLaser microdissectionComponents of pathwaysTranscription factor SPDEFFactor family membersMesenchymal-epithelial transitionKrüppel-like factor 4Regulatory networksRegulatory targetsRole of KLF4Spatiotemporal expressionQuantitative RT-PCRGlycoprotein biosynthesisMucosal epitheliumGoblet cellsGenesKLF4TranscriptsHigh Throughput Determination of TGFβ1/SMAD3 Targets in A549 Lung Epithelial Cells
Zhang Y, Handley D, Kaplan T, Yu H, Bais AS, Richards T, Pandit KV, Zeng Q, Benos PV, Friedman N, Eickelberg O, Kaminski N. High Throughput Determination of TGFβ1/SMAD3 Targets in A549 Lung Epithelial Cells. PLOS ONE 2011, 6: e20319. PMID: 21625455, PMCID: PMC3098871, DOI: 10.1371/journal.pone.0020319.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell LineChromatin ImmunoprecipitationDNA PrimersElectrophoretic Mobility Shift AssayEpithelial CellsHumansLungOligonucleotide Array Sequence AnalysisPromoter Regions, GeneticProtein BindingReverse Transcriptase Polymerase Chain ReactionSmad3 ProteinTransforming Growth Factor beta1ConceptsGene expression microarraysLung epithelial cellsMolecular pathwaysTranscriptional regulationExpression microarraysGlobal transcriptional regulationTGFβ1/Smad3Epithelial cellsHuman promoter regionsSignal transduction cascadeTarget gene expressionEpithelial cell phenotypeGene expression analysisTranscription factor Smad3Primary lung epithelial cellsSmad3 targetsQuantitative real-time RT-PCRFOXA2 promoterHuman A549 alveolar epithelial cellsChromatin immunoprecipitationTransduction cascadeTarget genesA549 lung epithelial cellsExpression analysisGene expression
2009
WNT5A Is a Regulator of Fibroblast Proliferation and Resistance to Apoptosis
Vuga LJ, Ben-Yehudah A, Kovkarova-Naumovski E, Oriss T, Gibson KF, Feghali-Bostwick C, Kaminski N. WNT5A Is a Regulator of Fibroblast Proliferation and Resistance to Apoptosis. American Journal Of Respiratory Cell And Molecular Biology 2009, 41: 583-589. PMID: 19251946, PMCID: PMC2778165, DOI: 10.1165/rcmb.2008-0201oc.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBeta CateninBlotting, WesternCase-Control StudiesCaspase 3Cell LineCell ProliferationCell SurvivalFibroblastsFibronectinsGene Expression ProfilingHumansHydrogen PeroxideIdiopathic Pulmonary FibrosisIntegrin alpha5LungMiceOligonucleotide Array Sequence AnalysisProto-Oncogene ProteinsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionRNA InterferenceTransfectionUp-RegulationWnt ProteinsWnt-5a ProteinConceptsUsual interstitial pneumoniaNormal lung fibroblastsLung tissueLung fibroblastsFibrotic interstitial lung diseaseInterstitial lung fibrosisSpecific histopathologic patternIdiopathic pulmonary fibrosisInterstitial lung diseaseRole of Wnt5aReal-time RT-PCRQuantitative real-time RT-PCRInterstitial pneumoniaPulmonary fibrosisAutoimmune diseasesHistopathologic patternLung diseaseLung fibrosisHistological patternNormal histologyWnt/beta-catenin pathwayCanonical Wnt/beta-catenin pathwayWestern blotFibroblast proliferationBeta-catenin pathway
2007
Effects of exercise training on quadriceps muscle gene expression in chronic obstructive pulmonary disease
Radom-Aizik S, Kaminski N, Hayek S, Halkin H, Cooper DM, Ben-Dov I. Effects of exercise training on quadriceps muscle gene expression in chronic obstructive pulmonary disease. Journal Of Applied Physiology 2007, 102: 1976-1984. PMID: 17483440, DOI: 10.1152/japplphysiol.00577.2006.Peer-Reviewed Original ResearchMeSH KeywordsAgedCase-Control StudiesCluster AnalysisEnergy MetabolismExerciseGene ExpressionGene Expression ProfilingHumansMaleOligonucleotide Array Sequence AnalysisOxidative StressOxygen ConsumptionProteasome Endopeptidase ComplexPulmonary Disease, Chronic ObstructiveQuadriceps MuscleReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerUbiquitinConceptsChronic obstructive pulmonary diseaseObstructive pulmonary diseaseCOPD patientsPulmonary diseaseExercise trainingAge-matched healthy menMuscle gene expressionHigh expressionSkeletal muscle functionExercise capacityGene expressionWalk testHealthy menControl subjectsNeedle biopsyMuscle functionVastus lateralisPatientsOxidative stressTraining responseFunctional parametersDiseaseTissue stressExpressionGene pathwaysChromosomal aberrations and gene expression profiles in non-small cell lung cancer
Dehan E, Ben-Dor A, Liao W, Lipson D, Frimer H, Rienstein S, Simansky D, Krupsky M, Yaron P, Friedman E, Rechavi G, Perlman M, Aviram-Goldring A, Izraeli S, Bittner M, Yakhini Z, Kaminski N. Chromosomal aberrations and gene expression profiles in non-small cell lung cancer. Lung Cancer 2007, 56: 175-184. PMID: 17258348, DOI: 10.1016/j.lungcan.2006.12.010.Peer-Reviewed Original ResearchMeSH KeywordsCarcinoma, Non-Small-Cell LungChromosome AberrationsGene ExpressionGene Expression ProfilingHumansLung NeoplasmsNucleic Acid HybridizationReverse Transcriptase Polymerase Chain ReactionConceptsDNA replicationCopy numberWnt/beta-catenin pathwaySet of genesGene Ontology annotationsFunctional enrichment analysisExpression levelsAmplification of 3qGene expression profilesBeta-catenin pathwayGene expression analysisGene expression profilingArray CGHMatrix metalloproteasesGene expression levelsConcordant changesCopy number changesDNA copy numberActing genesArray comparative genomic hybridizationOntology annotationsComparative genomic hybridizationGenomic contentReal-time quantitative PCRExpression analysis
2006
Comprehensive Analysis of Gene Expression on GOLD-2 Versus GOLD-0 Smokers Reveals Novel Genes Important in the Pathogenesis of COPD
Ning W, Lee J, Kaminski N, Feghali-Bostwick CA, Watkins SC, Pilewski JM, Peters DG, Hogg JC, Choi AM. Comprehensive Analysis of Gene Expression on GOLD-2 Versus GOLD-0 Smokers Reveals Novel Genes Important in the Pathogenesis of COPD. Annals Of The American Thoracic Society 2006, 3: 466-466. PMID: 16921106, DOI: 10.1513/pats.200603-031ms.Peer-Reviewed Original ResearchDNAExtracellular Matrix ProteinsGene ExpressionHumansIntercellular Signaling Peptides and ProteinsPulmonary Disease, Chronic ObstructiveReverse Transcriptase Polymerase Chain ReactionSmokingTranscription Factors
2005
Effects of Aerobic Training on Gene Expression in Skeletal Muscle of Elderly Men
RADOM-AIZIK S, HAYEK S, SHAHAR I, RECHAVI G, KAMINSKI N, BEN-DOV I. Effects of Aerobic Training on Gene Expression in Skeletal Muscle of Elderly Men. Medicine & Science In Sports & Exercise 2005, 37: 1680-1696. PMID: 16260967, DOI: 10.1249/01.mss.0000181838.96815.4d.Peer-Reviewed Original ResearchMeSH KeywordsAgedDown-RegulationExerciseExercise TestGene ExpressionHumansMaleMuscle, SkeletalOligonucleotide Array Sequence AnalysisOxygen ConsumptionPhysical FitnessReverse Transcriptase Polymerase Chain ReactionConceptsGene expressionGlobal gene expressionSkeletal muscleExpression of genesCatabolism genesTranscriptional responseHeme biosynthesisMonths of trainingElderly menGeneChip arraysGenesEnergy metabolismHealthy elderly menPeak O2 uptakeExpressionProtein catabolismPathwayAerobic trainingNeedle biopsyPhysical activityEnergy pathwaysVastus lateralisBeneficial effectsDephosphorylationRibosomes
2004
Gene expression profiling of in vivo UVB‐irradiated human epidermis
Enk CD, Shahar I, Amariglio N, Rechavi G, Kaminski N, Hochberg M. Gene expression profiling of in vivo UVB‐irradiated human epidermis. Photodermatology Photoimmunology & Photomedicine 2004, 20: 129-137. PMID: 15144390, DOI: 10.1111/j.1600-0781.2004.00097.x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultCase-Control StudiesDNA PrimersFemaleForearmGene Expression RegulationHumansMaleReverse Transcriptase Polymerase Chain ReactionSkinUltraviolet RaysConceptsGlobal gene expression analysisGene expression patternsGene expression analysisGene expression profilingIntact human epidermisOligonucleotide microarray technologyNumerous genesFunctional categoriesExpression analysisExpression profilingExpression patternsGene expressionBiological processesMicroarray profilingDifferential expressionDifferential regulationMicroarray technologyGenesMolecular pathwaysCultured cellsRNA samplesHuman epidermisUV-induced photodamageSemi-quantitative reverse transcriptase-polymerase chain reactionIntact tissue