2011
FACS purification of immunolabeled cell types from adult rat brain
Guez-Barber D, Fanous S, Harvey BK, Zhang Y, Lehrmann E, Becker KG, Picciotto MR, Hope BT. FACS purification of immunolabeled cell types from adult rat brain. Journal Of Neuroscience Methods 2011, 203: 10-18. PMID: 21911005, PMCID: PMC3221768, DOI: 10.1016/j.jneumeth.2011.08.045.Peer-Reviewed Original ResearchConceptsFluorescence-activated cell sortingCell typesPromoter-driven reporter geneBrain tissueExtracellular proteinsFACS procedureReporter geneFACS purificationRat brainReal-time PCRMolecular analysisSorted cellsCell sortingAdult rat brainTime PCRIntact cell bodiesTransgenic miceMolecular alterationsNeuN antibodyQuantitative assayCell bodiesAvailable antibodiesBrainTissueGenes
1995
Promoter elements conferring neuron-specific expression of the β2-subunit of the neuronal nicotinic acetylcholine receptor studiedin vitro and in transgenic mice
Bessis A, Salmon A, Zoli M, Le Novère N, Picciotto M, Changeux J. Promoter elements conferring neuron-specific expression of the β2-subunit of the neuronal nicotinic acetylcholine receptor studiedin vitro and in transgenic mice. Neuroscience 1995, 69: 807-819. PMID: 8596650, DOI: 10.1016/0306-4522(95)00303-z.Peer-Reviewed Original ResearchConceptsSpatio-temporal expression patternsFunctional E-boxesCell-specific transcriptionNeuron-specific transcriptionSite-directed mutagenesisTransient transfection assaysNeuron-specific expressionNeuronal genesRE1 sequenceTranscribed regionDeletion analysisE-boxRegulatory elementsPromoter elementsUpstream sequencesTransgenic miceTransfection assaysReporter geneRepressor elementExpression patternsNeuronal nicotinic acetylcholine receptorsGenesNicotinic acetylcholine receptorsRegulatory propertiesTranscription
1985
Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides
Schaefer M, Picciotto M, Kreiner T, Kaldany R, Taussig R, Scheller R. Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides. Cell 1985, 41: 457-467. PMID: 3838698, DOI: 10.1016/s0092-8674(85)80019-2.Peer-Reviewed Original ResearchConceptsAlternate RNA splicingAplysia nervous systemLys-Arg residuesHaploid genomeFMRFamide neuropeptidesRNA splicingCDNA clonesAnimal kingdomCDNA libraryMultiple transcriptsSingle copyCarboxy terminusAmino terminusInvertebrate tissuesFMRFamide peptidesDifferent tissuesPrecursor proteinPeptide Phe-MetGenesTerminusAplysia neuronsResiduesPhe-MetVariety of effectsArg-Phe